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Nuclear reprogramming: the zygotic transcription program is established through an“erase-and-rebuild” strategy 被引量:5
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作者 Feng Sun haiyan fang +5 位作者 Ruizhen Li Tianlong Gao Junke Zheng Xuejin Chen Wenqin Ying Hui Z Sheng 《Cell Research》 SCIE CAS CSCD 2007年第2期117-134,共18页
卵母细胞显示母亲特定的基因表达式侧面,它被换到当染色质的一个单倍体集合被传递到发展成一个胚胎的受精卵时, zygotic 介绍。位于这抄写 reprogramming 下面的机制当前是未知的。等到当抄写在幼芽的泡卵母细胞被关掉时,一般抄写因... 卵母细胞显示母亲特定的基因表达式侧面,它被换到当染色质的一个单倍体集合被传递到发展成一个胚胎的受精卵时, zygotic 介绍。位于这抄写 reprogramming 下面的机制当前是未知的。等到当抄写在幼芽的泡卵母细胞被关掉时,一般抄写因素和 transcriptional 管理者的一个范围从染色质被分裂,这里,我们表明那。染色质因素(CF ) 的全球分离破坏在染色质和 CF 之间的物理接触并且在功能的水平导致母亲的抄写节目的檫除。与染色质论坛分开的批评抄写因素和管理者遗体延长了时期,并且立即在专业版以后与染色质成为 re-associated 原子形成。Thisis 由象 DNA 和抄写那样的原子功能的重建跟随了速度集合。我们建议母亲的抄写节目在卵子发生期间被擦掉产生相对天真的染色质, zygotic 抄写节目是在授精以后的重建的重新。这个过程作为“ erase-and-rebuild ”被称为过程,它被用来重设抄写节目,和最可能的其它原子过程也,从母亲的到胚胎的。我们进一步在伴随的纸出现(高 T,等,房间物件 2007;17:135-150 .) 一样的策略也被采用到改编 transcriptional,这在体细胞介绍原子转移和单性生殖,建议这当模特儿在早胚胎开始期间对 transcriptional reprogramming 的所有形式普遍适用。从染色质的 CF 的排水量也为抄写沉默的现象提供解释在期间对 zygotic 转变母亲。 展开更多
关键词 核内程序重调 受精卵 合子 转录程序 擦除-重构策略 染色质 胚胎发生
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Effect of farmland shelterbelts on gully erosion in the black soil region of Northeast China 被引量:4
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作者 Rongxin Deng Wenjuan Wang +1 位作者 haiyan fang Zhihong Yao 《Journal of Forestry Research》 SCIE CAS CSCD 2015年第4期941-948,共8页
The black soil region of northeast China is one of the most important grain-producing areas in China. Increasingly severe gully erosion in this region has destroyed much farmland and reduced grain production. We analy... The black soil region of northeast China is one of the most important grain-producing areas in China. Increasingly severe gully erosion in this region has destroyed much farmland and reduced grain production. We analyzed SPOT5 imagery from 2007 and TM imagery from 2008 to describe the distributions of gullies and farmland shelterbelts in Kedong County and to assess the effect of farmland shelterbelts on gully erosion. The ima- gery revealed 2311 gullies with average density of 418.51 m km-2, indicating very serious gully erosion. With increasing slope gradient there was an inverse trend between gully density and shelterbelt density, indicating that farmland shelterbelts can prevent gully erosion. The defense effect of farmland shelterbelts against gullyerosion varied with distance: for distances 〈120 m, the defense effect was consistent and very strong; for distances of 120-240 m, a weak linear decrease was found in the defense effect; and for distances 〉240 m, the defense effect of the shelterbelts was significantly weaker. We recommend an optimal planting density of farmland shel- terbelts for the prevention of gully erosion at 1100-1300 m km-2. 展开更多
关键词 Black soil region Gully density Gullyerosion Farmland shelterbelts Northeast China
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Nuclear reprogramming: the strategy used in normal development is also used in somatic cell nuclear transfer and parthenogenesis 被引量:1
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作者 Tianlong Gao Junke Zheng +7 位作者 Fengying Xing haiyan fang Feng Sun Ayong Yan Xun Gong Hui Ding Fan Tang Hui Z Sheng 《Cell Research》 SCIE CAS CSCD 2007年第2期135-150,共16页
体细胞原子转移(SCNT ) 和单性生殖是复制和开发的其他的形式,造区分的体细胞原子核上的新生活周期并且分别地复制母亲的染色质。在前面的纸(阳光 F,等, CellRes 2007;17:117-134 .) ,我们显示出那“ erase-and-rebuild ”策略在... 体细胞原子转移(SCNT ) 和单性生殖是复制和开发的其他的形式,造区分的体细胞原子核上的新生活周期并且分别地复制母亲的染色质。在前面的纸(阳光 F,等, CellRes 2007;17:117-134 .) ,我们显示出那“ erase-and-rebuild ”策略在正常开发被使用转变母亲的基因表达式侧面到 zygotic。这里,如果一样的策略也适用于 SCNT 和单性生殖,我们调查。在染色质和染色质因素(CF ) 之间的关系用免疫化学的 andGFP 熔化蛋白质试金在 SCNT 和单性生殖期间被检验。从这些研究的结果显示了那此后不久原子的转移, CF 的一个多数从体的原子核分裂了并且被再分配到鸡蛋的细胞质。在卵子发生的檫除过程在 SCNT 在起始的阶段期间被重获。立即在他们的形成以后的大多数 CF enteredpseudo-pronuclei。在单性生殖,所有 parthenogenotes 经历了正常卵子发生,并且因此在开发的开始前把大多数 CF 从染色体移开。CF 是随后有女职业人员原子核的 re-associated 及时并且在使肥沃的胚胎的类似于那的顺序。把数据基于这些,我们断定进程在正常开发观察了的“ erase-and-rebuild ”也发生在 SCNT 并且在单性生殖,以改变的方式的虽然。这个过程为在这些的抄写 reprogramming 负责过程。在 SCNT “擦掉”过程被压缩,效率被损害,它多半贡献经常在原子转移(nt ) 观察的发展缺点胚胎。而且,,从这研究的结果显示一颗卵的细胞质包含大多数并非所有,为装配 zygotic 的必要部件编程序并且能装配他们到不同起源的适当双染色质上。 展开更多
关键词 核内程序重调 体细胞核移植 孤雌生殖 正常胚胎发育 染色质
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Denoising of X-ray pulsar observed profile using biorthogonal lifting wavelet transform 被引量:3
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作者 Mengfan Xue Xiaoping Li +3 位作者 Yanming Liu haiyan fang Haifeng Sun Lirong Shen 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2016年第3期514-523,共10页
In X-ray pulsar-based navigation, strong X-ray background noise leads to a low signal-to-noise ratio(SNR) of the observed profile, which consequently makes it very difficult to obtain an accurate pulse phase that di... In X-ray pulsar-based navigation, strong X-ray background noise leads to a low signal-to-noise ratio(SNR) of the observed profile, which consequently makes it very difficult to obtain an accurate pulse phase that directly determines the navigation precision. This signifies the necessity of denoising of the observed profile. Considering that the ultimate goal of denoising is to enhance the pulse phase estimation, a profile denoising algorithm is proposed by fusing the biorthogonal lifting wavelet transform of the linear phase characteristic with the thresholding technique. The statistical properties of X-ray background noise after epoch folding are studied. Then a wavelet-scale dependent threshold is introduced to overcome correlations between wavelet coefficients. Moreover, a modified hyperbola shrinking function is presented to remove the impulsive oscillations of the observed profile. The results of numerical simulations and real data experiments indicate that the proposed method can effectively improve SNR of the observed profile and pulse phase estimation accuracy, especially in short observation durations. And it also outperforms the Donoho thresholding strategy normally used in combination with the orthogonal discrete wavelet transform. 展开更多
关键词 X-ray pulsar denoising linear phase wavelet-scale dependent threshold
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Relationship between morphospecies and microcystin-producing genotypes of Microcystis species in Chinese freshwaters
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作者 Yang LIU Youxin CHEN +5 位作者 haiyan fang Hanyang LU Xingqiang WU Gongliang YU Shin-ichi NAKANO Renhui LI 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2021年第5期1926-1937,共12页
Twenty water bodies in China were sampled,and 186 strains of different Microcystis species were isolated,from which eight morpho species were identified and 43 stains containing the mcyB gene were detected.Phylogeneti... Twenty water bodies in China were sampled,and 186 strains of different Microcystis species were isolated,from which eight morpho species were identified and 43 stains containing the mcyB gene were detected.Phylogenetic analysis based on the mcyB gene indicated that the microcystin(MC)-producing Microcystis in China could be divided into two groups(ⅠandⅡ)and showed significant differences between the two groups.The maximum sequence similarity was 69.1%.Microcystins(MCs)were measured by high-performance liquid chromatography(HPLC)analysis,and no microcystin-RR(MC-RR)was detected in some strains belonging to GroupⅡ.Compared to other regions of the world,the proportion of Chinese MC-producing was different,and the regional differences were more obvious.A whole-cell polymerase chain reactio(PCR)assay was conducted to analyze the proportion of the mcyB gene in the laboratory cultured and field cultured Microcystis.The proportion of four morphospecies(M.vividis,M.ichthyoblabe,M.novacekii,and M.aeruginosa)that contained the mcyB gene exceeded 50%in the field cultured sample s.Compared with former studies,M.aeruginosa was the mo st likely morphotype that can produce MCs in the world.This study provided new insight of Microcystis hazard assessment and field monitoring. 展开更多
关键词 MICROCYSTIS MORPHOSPECIES mcyB 16S rDNA MICROCYSTIN high-performance liquid chromatography(HPLC)
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青藏高原河川径流变化及其影响研究进展 被引量:45
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作者 汤秋鸿 兰措 +15 位作者 苏凤阁 刘星才 孙赫 丁劲 王磊 冷国勇 张永强 桑燕芳 方海燕 张士锋 韩冬梅 刘小莽 贺莉 徐锡蒙 唐寅 Deliang Chen 《科学通报》 EI CAS CSCD 北大核心 2019年第27期2807-2821,共15页
青藏高原被称为世界'第三极',又有'亚洲水塔'之称,对其周边地区的水文和气候系统有重要影响.青藏高原是亚洲许多大河的发源地,其冰川与河川径流变化影响到周边数十亿人口.本文介绍了青藏高原河川径流观测现状,回顾了青... 青藏高原被称为世界'第三极',又有'亚洲水塔'之称,对其周边地区的水文和气候系统有重要影响.青藏高原是亚洲许多大河的发源地,其冰川与河川径流变化影响到周边数十亿人口.本文介绍了青藏高原河川径流观测现状,回顾了青藏高原河川径流变化研究. 20世纪50年代至21世纪初,黄河源区年径流呈减少趋势、长江源区年径流呈微弱增加趋势,青藏高原其他江河源区的年径流没有显著的变化趋势.黄河上游、澜沧江上游、沱沱河及拉萨河源区的春季径流有增加趋势.未来气候变化情景下,随着降水和冰雪融水增加,青藏高原大部分河流源区径流增加,洪水等极端水文事件发生更加频繁.青藏高原河流源区水文气象观测资料稀缺,是河川径流变化及其影响研究的重大挑战.青藏高原水文研究亟需结合最新观测与模拟技术,提高水循环观测与模拟能力,深入认识青藏高原河川径流复杂性及其变化规律,为径流变化的影响评估及其应对提供科技支撑. 展开更多
关键词 青藏高原 亚洲水塔 水循环 河川径流 气候变化
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