Src SH3 protein domain is a typical two-state protein which has been confirmed by research of denaturant-induced unfolding dynamics.Force spectroscopy experiments by optical tweezers and atomic force microscopy have m...Src SH3 protein domain is a typical two-state protein which has been confirmed by research of denaturant-induced unfolding dynamics.Force spectroscopy experiments by optical tweezers and atomic force microscopy have measured the force-dependent unfolding rates with different kinds of pulling geometry.However,the equilibrium folding and unfolding dynamics at constant forces has not been reported.Here,using stable magnetic tweezers,we performed equilibrium folding and unfolding dynamic measurement and force-jump measurement of src SH3 domain with tethering points at its N-and C-termini.From the obtained force-dependent transition rates,a detailed two-state free energy landscape of src SH3 protein is constructed with quantitative information of folding free energy,transition state barrier height and position,which exemplifies the capability of magnetic tweezers to study protein folding and unfolding dynamics.展开更多
Phasor measurement units(PMUs)provide useful data for real-time monitoring of the smart grid.However,there may be time-varying deviation in phase angle differences(PADs)between both ends of the transmission line(TL),w...Phasor measurement units(PMUs)provide useful data for real-time monitoring of the smart grid.However,there may be time-varying deviation in phase angle differences(PADs)between both ends of the transmission line(TL),which may deteriorate application performance based on PMUs.To address that,this paper proposes two robust methods of correcting time-varying PAD deviation with unknown parameters of TL(ParTL).First,the phenomena of time-varying PAD deviation observed from field PMU data are presented.Two general formulations for PAD estimation are then established.To simplify the formulations,estimation of PADs is converted into the optimal problem with a single ParTL as the variable,yielding a linear estimation of PADs.The latter is used by second-order Taylor series expansion to estimate PADs accurately.To reduce the impact of possible abnormal amplitude data in field data,the IGG(Institute of Geodesy&Geophysics,Chinese Academy of Sciences)weighting function is adopted.Results using both simulated and field data verify the effectiveness and robustness of the proposed methods.展开更多
基金the National Natural Science Foundation of China(Grant Nos.11874309 and 11474237)the 111 Project(Grant No.B16029)。
文摘Src SH3 protein domain is a typical two-state protein which has been confirmed by research of denaturant-induced unfolding dynamics.Force spectroscopy experiments by optical tweezers and atomic force microscopy have measured the force-dependent unfolding rates with different kinds of pulling geometry.However,the equilibrium folding and unfolding dynamics at constant forces has not been reported.Here,using stable magnetic tweezers,we performed equilibrium folding and unfolding dynamic measurement and force-jump measurement of src SH3 domain with tethering points at its N-and C-termini.From the obtained force-dependent transition rates,a detailed two-state free energy landscape of src SH3 protein is constructed with quantitative information of folding free energy,transition state barrier height and position,which exemplifies the capability of magnetic tweezers to study protein folding and unfolding dynamics.
基金This work was supported by the National Key Research and Development Program of China(2017YFB0902901)National Natural Science Foundation of China(51627811).
文摘Phasor measurement units(PMUs)provide useful data for real-time monitoring of the smart grid.However,there may be time-varying deviation in phase angle differences(PADs)between both ends of the transmission line(TL),which may deteriorate application performance based on PMUs.To address that,this paper proposes two robust methods of correcting time-varying PAD deviation with unknown parameters of TL(ParTL).First,the phenomena of time-varying PAD deviation observed from field PMU data are presented.Two general formulations for PAD estimation are then established.To simplify the formulations,estimation of PADs is converted into the optimal problem with a single ParTL as the variable,yielding a linear estimation of PADs.The latter is used by second-order Taylor series expansion to estimate PADs accurately.To reduce the impact of possible abnormal amplitude data in field data,the IGG(Institute of Geodesy&Geophysics,Chinese Academy of Sciences)weighting function is adopted.Results using both simulated and field data verify the effectiveness and robustness of the proposed methods.