创伤后成长的研究热点和主题趋势:基于关键词的共词分析

目的 采用基于关键词的共词分析方法,分析、总结近15年创伤后成长(post-traumatic growth,PTG)的研究热点和进展趋势,为PTG的相关研究和临床干预提供参考依据.方法 在PubMed和Web of Science数据库中检索于2013年1月至2022年7月发表的与PTG相关的研究文献.采用共11个医学主题词(Medical Subject Headings,MeSH)和关键词来检索、选择研究文献.使用书目共现分析系统(Bibliographic Item Co-occurrence Matrix Builder,BICOMB 2.0)提取高频主题词并生成词篇矩阵,使用图形聚类工具包(Graphical Clustering Toolkit,gCLUTO 1.0)进行聚类分析,使用SPSS 25.0进行战略图分析.结果 共筛选出2 370篇文献,提取38个高频主题词.研究结果揭示了 2013-2022年关于PTG的6个研究热点:遭遇负性生活事件后的情绪反应研究,癌症患者的PTG研究,经历创伤后的反刍和韧性研究,儿童和青少年的PTG研究,社会支持和应对策略在PTG中的作用研究,以及PTG与生活质量之间的关系研究.结论 采用共词分析方法,有效揭示了近15年PTG相关研究的概况.本研究中揭示的6个研究类别既反映了PTG研究领域的多样性,也说明部分研究主题尚未成熟.本研究结果对未来开展与PTG相关的研究具有重要参考价值.

Uterine artery pseudoaneurysm after subtotal hysterectomy:a case report

Vaginal bleeding is a common symptom in the emergency department of obstetrics and gynecology hospitals;however,post-hysterectomy vaginal bleeding is a rare phenomenon.Uterine artery pseudoaneurysm(UAP)is a rare condition that can lead to fatal bleeding and most commonly occurs as a long-term complication after invasive surgery or even in non-invasive procedures,such as cesarean section,myomectomy,dilatation and curettage,cervical conization and vaginal delivery.[1-3]Pseudoaneurysm is a pulsatile hematoma formed after arterial injury and rupture.

Changes and Influencing Factors of Maize Production Pattern in China

Since the founding of new China,the maize production pattern took on characteristic of " northern expansion and western movement" : there were great changes in ranking of main producing provinces; southern planting area gradually shrank,while central and northern planting area gradually expanded; apart from traditional main producing regions such as northeastern,north China,and southwestern regions,Shanxi- Shaanxi region and northwest region are gradually forming. To further analyze factors promoting changes in maize production pattern,based on 1987- 2013 panel data,we carried our empirical study and obtained five influencing factors: resource endowment,economic environment,market environment,technical conditions,and policies. Per capita farmland,multiple cropping index,water conservancy and irrigation,and benefit cost ratio,and traffic and transportation policies exert positive effect,non-agricultural employment level exerts negative effect,and the significance of natural disasters,market price,and science and technology are inadequate.

SARS-CoV-2 spike protein receptor-binding domain perturbates intracellular calcium homeostasis and impairs pulmonary vascular endothelial cells

Exposure to the spike protein or receptor-binding domain(S-RBD)of SARS-CoV-2 significantly influences endothelial cells and induces pulmonary vascular endotheliopathy.In this study,angiotensin-converting enzyme 2 humanized inbred(hACE2 Tg)mice and cultured pulmonary vascular endothelial cells were used to investigate how spike protein/S-RBD impacts pulmonary vascular endothelium.Results show that S-RBD leads to acute-to-prolonged induction of the intracellular free calcium concentration([Ca^(2+)]i)via acute activation of TRPV4,and prolonged upregulation of mechanosensitive channel Piezo1 and store-operated calcium channel(SOCC)key component Orai1 in cultured human pulmonary arterial endothelial cells(PAECs).In mechanism,S-RBD interacts with ACE2 to induce formation of clusters involving Orai1,Piezo1 and TRPC1,facilitate the channel activation of Piezo1 and SOCC,and lead to elevated apoptosis.These effects are blocked by Kobophenol A,which inhibits the binding between S-RBD and ACE2,or intracellular calcium chelator,BAPTA-AM.Blockade of Piezo1 and SOCC by GsMTx4 effectively protects the S-RBDinduced pulmonary microvascular endothelial damage in hACE2 Tg mice via normalizing the elevated[Ca^(2+)]i.Comparing to prototypic strain,Omicron variants(BA.5.2 and XBB)of S-RBD induces significantly less severe cell apoptosis.Transcriptomic analysis indicates that prototypic S-RBD confers more severe acute impacts than Delta or Lambda S-RBD.In summary,this study provides compelling evidence that S-RBD could induce persistent pulmonary vascular endothelial damage by binding to ACE2 and triggering[Ca^(2+)]i through upregulation of Piezo1 and Orai1.Targeted inhibition of ACE2-Piezo1/SOCC-[Ca^(2+)]i axis proves a powerful strategy to treat S-RBD-induced pulmonary vascular diseases.

SARS-CoV-2 spike protein induces IL-18-mediated cardiopulmonary inflammation via reduced mitophagy

Cardiopulmonary complications are major drivers of mortality caused by the SARS-CoV-2 virus.Interleukin-18,an inflammasomeinduced cytokine,has emerged as a novel mediator of cardiopulmonary pathologies but its regulation via SARS-CoV-2 signaling remains unknown.Based on a screening panel,IL-18 was identified amongst 19 cytokines to stratify mortality and hospitalization burden in patients hospitalized with COVID-19.Supporting clinical data,administration of SARS-CoV-2 Spike 1(S1)glycoprotein or receptor-binding domain(RBD)proteins into human angiotensin-converting enzyme 2(hACE2)transgenic mice induced cardiac fibrosis and dysfunction associated with higher NF-κB phosphorylation(pNF-κB)and cardiopulmonary-derived IL-18 and NLRP3 expression.IL-18 inhibition via IL-18BP resulted in decreased cardiac pNF-κB and improved cardiac fibrosis and dysfunction in S1-or RBD-exposed hACE2 mice.Through in vivo and in vitro work,both S1 and RBD proteins induced NLRP3 inflammasome and IL-18 expression by inhibiting mitophagy and increasing mitochondrial reactive oxygenation species.Enhancing mitophagy prevented Spike protein-mediated IL-18 expression.Moreover,IL-18 inhibition reduced Spike protein-mediated pNF-κB and EC permeability.Overall,the link between reduced mitophagy and inflammasome activation represents a novel mechanism during COVID-19 pathogenesis and suggests IL-18 and mitophagy as potential therapeutic targets.

Allene oxide synthase 1 contributes to limiting grain arsenic accumulation and seedling detoxification in rice

Arsenic(As)is a cancerogenic metalloid ubiquitously distributed in the environment,which can be easily accumulated in food crops like rice.Jasmonic acid(JA)and its derivatives play critical roles in plant growth and stress response.However,the role of endogenous JA in As accumulation and detoxification is still poorly understood.In this study,we found that JA biosynthesis enzymes Allene Oxide Synthases,OsAOS1 and OsAOS2,regulate As accumulation and As tolerance in rice.Evolutionary bioinformatic analysis indicated that AOS1 and AOS2 have evolved from streptophyte algae(e.g.the basal lineage Klebsormidium flaccidum)-sister clade of land plants.Compared to other two AOSs,OsAOS1 and OsAOS2 were highly expressed in all examined rice tissues and their transcripts were highly induced by As in root and shoot.Loss-of-function of OsAOS1(osaos1-1)showed elevated As concentration in grains,which was likely attributed to the increased As translocation from root to shoot when the plants were subjected to arsenate[As(Ⅴ)]but not arsenite[As(Ⅲ)].However,the mutation of OsAOS2(osaos2-1)showed no such effect.Moreover,osaos1-1 and osaos2-1 increased the sensitivity of rice plants to both As(Ⅴ)and As(Ⅲ).Disrupted expression of genes involved in As accumulation and detoxification,such as OsPT4,OsNIP3;2,and OsOASTL-A1,was observed in both osaos1-1 and osaos2-1 mutant lines.In addition,a As(Ⅴ)-induced significant decrease in Reactive Oxygen Species(ROS)production was observed in the root of osaos1-1 but not in osaos2-1.Taken together,our results indicate OsAOS1 modulates both As allocation and detoxification,which could be partially attributed to the altered gene expression profiling and ROS homeostasis in rice while OsAOS2 is important for As tolerance.