Insight into the quality deterioration induced by microorganisms in Litopenaeus vannamei during superchilling storage

Quality deterioration induced by microbial community in Litopenaeus vannamei during superchilling storage were demonstrated in this study.In this study,6 microorganisms were identified as the biomarkers by 16S rDNA sequencing and 71 differential metabolites were identified by untargeted metabolomics.The correlation analysis between microorganisms and differential metabolites revealed that Oceanicaulis,Aliihoeflea,Prauserella,Chelativorans and Pseudoalteromonas had the potential to be the important microorganisms of deterioration of superchilled shrimp.Moreover,the spearman correlation analysis revealed that lightness value had highly significant correlation with Shewanella(P<0.01),and thiobarbituric acid content,total viable count value,total sulfhydryl content,trichloroacetic acid-soluble peptide and Ca2+-ATPase activity showed significantly correlation with Oceanicaulis,Prauserella,Chelativorans,Aliihoefl ea and Pseudoalteromonas(P<0.05).This study offers a better understanding for mechanisms behind quality degradation induced by microorganisms,which may help the quality maintaining of Pacific white shrimp during superchilling storage.

Chinese herbal medicine for treatment of dislipidemia

Prevalence of dislipidemia is increasing rapidly in China and there has been a growing interest in Chinese herbal medicine for the treatment of hyperlipidemia both inside and outside China. In this article, lipids regulating effects of 9 herbs or their extracts and 5 herbal formulae which have been published in English-language literature are reviewed. Although evidence from animals and humans consistently supports the therapeutic activities of these Chinese herbal medicines, few multi-center large-scale clinical trials have been conducted to confirm the efficacy and evaluate their safety.

Feasibility and safety of combined interventional procedures in elderly patients with complex cardiovascular diseases:experience of a single medical center

Objectives:To report the clinical experience of combined interventional procedures in the treatment of elderly patients with coexisting two or more cardiovascular diseases in our medical center,and to assess the feasibility,safety and therapeutic efficacy of this management strategy.Methods:Patients were selected to the study if:1)age>65 years;2)with coexistence of two or more cardiovascular diseases which are indications for interventional therapy;3)patients'general condition and organ functions allow the performance of combined multiple procedures;4)the predicted procedure time is within 150 min;5)the predicted contrast medium dosage is within 300 ml.The criteria we analyzed included procedural type,rocedural time,fluoroscopy time,dosage of contrast medium,success rates of the procedures,complications and in-hospital mortality.All patients were followed up for 30.4±9.3 months,to determine the all-cause mortality,recurrence rates and adverse cardiac events.Results:From January 2000 to December 2004,combined interventional procedures were performed on 136 patients,with 2 procedures on 134 patients and 3 procedures on 2 patients.The mean procedure time was 115.4±11.6 min,the mean fluoroscopy time was 35.7±9.3 min,and the mean dosage of contrast medium used was 183.6±19.4 ml.Procedural success rate was 100%,no procedure related death or major complications occurred.Conclusion:Performed by a competent team,combined interventional procedures in elderly patients with multiple cardiovascular diseases were feasible and relatively safe.

Smart control system for the precision cultivation of black fungus

Black fungus,with high nutritional and medicinal value,has been cultivated in China for a long time,and Heilongjiang alone accounts for about 40%of the global output.At present,the cultivation of black fungus derives mainly from the inheritance of relatively primitive practices and experience of farmers,resulting in inconsistent quality of fungus.In this study,a smart control system for the precision cultivation of black fungus was designed by using intelligent detection and control technology.The system includes a precision culture test environment and remote control system.The precision cultivation environment contains four sub-independent environments.The key parameters such as temperature,humidity,and light behavior were collected and can be adjusted individually,according to the precision cultivation stages.The intelligent remote control system included a controller cabinet,sensors unit,temperature control unit,humidity control unit,light control unit,and information transmitting unit.The controller cabinet includes a key controller which can auto-control the temperature,humidity,and lightly adjust components according to the precision cultivation conditions and processing.The temperature sensors were installed in a 3D array close to the fungus bags about 5 cm in rooms.The light tape was installed on the six walls and also had three colors(Red,Blue,and Green)which could be controlled independently in each room.The control strategy through the analysis of the data collected by all sensors,the current cultivate situation of the cultivation environment was obtained,and the heater,fan,light,and nozzle were regulated according to the strategy to maintain a suitable precision cultivation environment for fungus.To verify the feasibility of the precision cultivation processing and control system,the test result shows that the error of temperature control was about 0℃-1℃,the error of humidity control was about 1%-4%,and the error of illuminance control was about 0-50 lx;All the verification results show that the control system for precision cultivation has high precision and can meet the needs of exploring the"Black 29"fungus cultivation experiment environment.Based on the orthogonal experiment,the best combination of the temperature and humidity for each growth stage was also investigated in this study,further proving the reliability and feasibility of the control system for the precision cultivation of Auricularia auricula.

Analytical Determination of Interwell Connectivity Based on Interwell Influence

Interwell connectivity, an important element in reservoir characterization, especially for water flooding,is used to make decisions for better oil production. The existing methods in literature directly use related data of wells to infer interwell connectivity, but they ignore the influence between different wells. The connection of one well to more than two wells(as is often true in the oil field well pattern) will impact the accuracy of the connectivity analysis. To address this challenge, this paper proposes the Particle Swarm Optimization-based CatBoost for Interwell Connectivity(PSOC4IC) based on relative features to analyze interwell connectivity with the combination of joint mutual information maximization-based denoising sparse autoencoder for inter-feature construction and extraction and PSO-based CatBoost(PSO-CatBoost) for connectivity prediction with high-dimensional noise data.The experimental results show that the PSOC4IC improves analysis accuracy.