Objective: To determine Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) isolated from periodontal patients and healthy subjects using culture and PCR methods. Methods: Duplicate paper point needles we...Objective: To determine Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) isolated from periodontal patients and healthy subjects using culture and PCR methods. Methods: Duplicate paper point needles were taken from 100 samples (50 healthy subjects and 50 patients), who referred to the specialized dental clinic from Oct. 2015 to Mar. 2016. In laboratory after incubation period and observing the star-shaped colony A. actinomycetemcomitans, the confirmation tests, including gram staining and catalase test were carried out. For PCR, samples were analyzed with genus specific primers. These primers set, amplified a 500 bp fragment. Results: Of the 100 samples, A. actinomycetemcomitans was isolated from 31 patients (31%), (24 isolate of patients, and 7 isolate of healthy subjects) by using a selective Aggregatibacter isolation medium. Using PCR, a total of 49 (49%) samples were found to be positive for A. actinomycetemcomitans (35 isolate of patients, and 14 isolate of healthy subjects). Conclusion: PCR was found to be highly sensitive when genus specific primers were used for diagnosis of A. actinomycetemcomitans in comparison with culture method.展开更多
Designing equipment for processing,sorting,sizing and other post-harvest operations of agricultural products requires information about their physical properties.This study was carried out to evaluate the effect of mo...Designing equipment for processing,sorting,sizing and other post-harvest operations of agricultural products requires information about their physical properties.This study was carried out to evaluate the effect of moisture content on some physical properties of barley grains.Four levels of moisture content ranging from 7.34%to 21.58%(d.b.)were used.The average length,width,thickness,arithmetic mean diameter,geometric mean diameter,thousand grain mass,sphericity,surface area and repose angle increased from 8.91 to 9.64 mm,3.30 to 3.74 mm,2.58 to 2.98 mm,4.93 to 5.45 mm,4.23 to 4.75 mm,44.48 to 51.30 g,47.55%to 49.35%,56.66 to 71.09 mm2 and 31.16°to 36.90°,respectively,as moisture content increased from 7.34%to 21.58%(d.b.).The bulk density,true density and porosity were found to decrease with increasing moisture content.The static friction coefficient of the grains increased linearly against various surfaces(plywood,glass and galvanized iron sheet)as the moisture content increased.At all moisture content,the maximum friction was offered by plywood,followed by galvanized iron sheet and glass surface.展开更多
文摘Objective: To determine Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) isolated from periodontal patients and healthy subjects using culture and PCR methods. Methods: Duplicate paper point needles were taken from 100 samples (50 healthy subjects and 50 patients), who referred to the specialized dental clinic from Oct. 2015 to Mar. 2016. In laboratory after incubation period and observing the star-shaped colony A. actinomycetemcomitans, the confirmation tests, including gram staining and catalase test were carried out. For PCR, samples were analyzed with genus specific primers. These primers set, amplified a 500 bp fragment. Results: Of the 100 samples, A. actinomycetemcomitans was isolated from 31 patients (31%), (24 isolate of patients, and 7 isolate of healthy subjects) by using a selective Aggregatibacter isolation medium. Using PCR, a total of 49 (49%) samples were found to be positive for A. actinomycetemcomitans (35 isolate of patients, and 14 isolate of healthy subjects). Conclusion: PCR was found to be highly sensitive when genus specific primers were used for diagnosis of A. actinomycetemcomitans in comparison with culture method.
文摘Designing equipment for processing,sorting,sizing and other post-harvest operations of agricultural products requires information about their physical properties.This study was carried out to evaluate the effect of moisture content on some physical properties of barley grains.Four levels of moisture content ranging from 7.34%to 21.58%(d.b.)were used.The average length,width,thickness,arithmetic mean diameter,geometric mean diameter,thousand grain mass,sphericity,surface area and repose angle increased from 8.91 to 9.64 mm,3.30 to 3.74 mm,2.58 to 2.98 mm,4.93 to 5.45 mm,4.23 to 4.75 mm,44.48 to 51.30 g,47.55%to 49.35%,56.66 to 71.09 mm2 and 31.16°to 36.90°,respectively,as moisture content increased from 7.34%to 21.58%(d.b.).The bulk density,true density and porosity were found to decrease with increasing moisture content.The static friction coefficient of the grains increased linearly against various surfaces(plywood,glass and galvanized iron sheet)as the moisture content increased.At all moisture content,the maximum friction was offered by plywood,followed by galvanized iron sheet and glass surface.
