AIM: To elucidate the relationship between apoptotic protease activating factor-1 (Apaf-1) gene and gastric cancer. METHODS: Thirty-five postoperative cancer and adjacent normal tissue samples were collected in the pr...AIM: To elucidate the relationship between apoptotic protease activating factor-1 (Apaf-1) gene and gastric cancer. METHODS: Thirty-five postoperative cancer and adjacent normal tissue samples were collected in the present study. Expression of the Apaf-1 gene in these samples was analyzed by semi-quantitative RT-PCR. Loss of heterozygosity (LOH) was used to determine whether there was loss of Apaf-1 gene in domain of 12q22-23 in the samples. Promoter methylation of Apaf-1 gene in the samples was analyzed by methylation specific (MSP) PCR. RESULTS: The expression of Apaf-1 mRNA in gastric cancer tissue samples was 51%. The LOH frequency of D12S346, D12S1706, D12S327, D12S1657 and D12S393 was 33%, 8%, 58%, 12% and 42%, respectively. Fifty percent LOH was found at two sites and 17% LOH at three sites. Apaf-1 mRNA expression decreased significantly in 13 cases (rs = 0.487, P = 0.003). The rate of Apaf-1 promoter methylation was 49% in gastric cancer tissue samples and 23% in para-cancerous tissue samples. Promoter methylation occurred significantly in 16 of 18 gastric cancer tissue samples with decreased expression of Apaf-1 mRNA rs = 0.886, P = 10-6). CONCLUSION: The expression of Apaf-1 gene is low in gastric cancer tissues. Methylation of Apaf-1 gene promoter and LOH in domain of 12q22-23 are the main reasons for the expression and altered expression of Apaf-1 gene.展开更多
AIM To investigate whether M1 or M2 polarization contributes to the therapeutic effects of mesenchymal stem cells(MSCs) in acute hepatic failure(AHF).METHODS MSCs were transfused into rats with AHF induced by D-galact...AIM To investigate whether M1 or M2 polarization contributes to the therapeutic effects of mesenchymal stem cells(MSCs) in acute hepatic failure(AHF).METHODS MSCs were transfused into rats with AHF induced by D-galactosamine(DGal N). The therapeutic effects of MSCs were evaluated based on survival rate and hepatocyte proliferation and apoptosis. Hepatocyte regeneration capacity was evaluated by the expression of the hepatic progenitor surface marker epithelial cell adhesion molecule(Ep CAM). Macrophage polarization was analyzed by M1 markers [CD68,tumor necrosis factor alpha(TNF-α),interferon-γ(IFN-γ),inducible nitric oxide synthase(INOS)] and M2 markers [CD163,interleukin(IL)-4,IL-10,arginase-1(Arg-1)] in the survival and death groups after MSC transplantation.RESULTS The survival rate in the MSC-treated group was increased compared with the DPBS-treated control group(37.5% vs 10%). MSC treatment protected rats with AHF by reducing apoptotic hepatocytes and promoting hepatocyte regeneration. Immunohistochemical analysis showed that MSC treatment significantly increased the expression of Ep CAM compared with the control groups(P < 0.001). Expression of Ep CAM in the survival group was significantly up-regulated compared with the death group after MSC transplantation(P = 0.003). Transplantation of MSCs significantly improved the expression of CD163 and increased the gene expression of IL-10 and Arg-1 in the survival group. IL-4 concentrations were significantly increased compared to the death group after MSC transplantation(88.51 ± 24.51 pg/m L vs 34.61 ± 6.6 pg/m L,P < 0.001). In contrast,macrophages showed strong expression of CD68,TNF-α,and INOS in the death group. The concentration of IFN-γ was significantly increased compared to the survival group after MSC transplantation(542.11 ± 51.59 pg/m L vs 104.07 ± 42.80 pg/m L,P < 0.001).CONCLUSION M2 polarization contributes to the therapeutic effects of MSCs in AHF by altering levels of anti-inflammatory and pro-inflammatory factors.展开更多
Bismuth ferrite(BiFeO3)-based materials are multiferroic materials widely studied.This study reports that strong ferroelectricity and enhanced magnetic performance are simultaneously obtained in the quenched(1−x)BiFeO...Bismuth ferrite(BiFeO3)-based materials are multiferroic materials widely studied.This study reports that strong ferroelectricity and enhanced magnetic performance are simultaneously obtained in the quenched(1−x)BiFeO3−xBaTiO3(BFBT100x,x=0.2 and 0.3)ceramics.Quenching treatment can reduce the amount of defects and Fe2+ions and make the defect dipole in a random state,which is conducive to improving the ferroelectricity and magnetism.Compared with the conventional sintered samples,the quenched ceramics have higher remnant and saturation polarization.As for magnetism,the coercive field(Hc)of the quenched ceramics is smaller and the quenching treatment can increase the maximum magnetization by up to 15%.展开更多
Multiferroic BiFeO_(3)-based ceramics were synthesized using the rapid liquid-phase sintering method.The rare-earth ion(Sm^(3+),Gd^(3+),Y^(3+))doping causes structural distortion without changing the intrinsic rhomboh...Multiferroic BiFeO_(3)-based ceramics were synthesized using the rapid liquid-phase sintering method.The rare-earth ion(Sm^(3+),Gd^(3+),Y^(3+))doping causes structural distortion without changing the intrinsic rhombohedral perovskite structure.Raman analysis shows that the effect of doping on E modes is greater than A1 modes,and the microstructure of FeO_(6) octahedron can be regulated by ion doping.A-site trivalent ion doped ceramics exhibit improved magnetism compared with pure BiFeO_(3) ceramic,which originated from the suppressed spiral spin structure of Fe ions.The tilt of FeO_(6) octahedron as a typical structure instability causes the anomalous change of the imaginary part of permittivity at high frequency,and doped ceramics exhibit natural resonance around 16-17 GHz.展开更多
The barium ferrite BaTi_(x)Fe_(12−x)O_(19)(x=0.2,0.4,0.6,0.8)(BFTO-x)ceramics doped by Ti4+were synthesized by a modified sol–gel method.The crystal structure and magnetic structure of the samples were determined by ...The barium ferrite BaTi_(x)Fe_(12−x)O_(19)(x=0.2,0.4,0.6,0.8)(BFTO-x)ceramics doped by Ti4+were synthesized by a modified sol–gel method.The crystal structure and magnetic structure of the samples were determined by neutron diffraction,and confirm that the BFTO-x ceramics were high quality single phase with sheet microstructure.With x increasing from 0.2 to 0.8,the saturation magnetization(M_(s))decreases gradually but the change trend of coercivity(H_(c))is complex under the synergy of the changed grain size and the magnetic crystal anisotropy field.Relying on the high valence of Ti^(4+),double resonance peaks are obtained in the curves of the imaginary part of magnetic conductivity(μ′′)and the resonance peaks could move toward the low frequency with the increase of x,which facilitate the samples perform an excellent wideband modulation microwave absorption property.In the x=0.2 sample,the maximum reflection loss(RL)can reach−44.9 dB at the thickness of only 1.8 mm,and the bandwidth could reach 5.28 GHz at 2 mm when RL is less than−10 dB.All the BFTO-x ceramics show excellent frequency modulation ability varying from 18(x=0.8)to 4 GHz(x=0.4),which covers 81%of the investigated frequency in microwave absorption field.This work not only implements the tunable of electromagnetic parameters but also broadens the application of high-performance microwave absorption devices.展开更多
文摘AIM: To elucidate the relationship between apoptotic protease activating factor-1 (Apaf-1) gene and gastric cancer. METHODS: Thirty-five postoperative cancer and adjacent normal tissue samples were collected in the present study. Expression of the Apaf-1 gene in these samples was analyzed by semi-quantitative RT-PCR. Loss of heterozygosity (LOH) was used to determine whether there was loss of Apaf-1 gene in domain of 12q22-23 in the samples. Promoter methylation of Apaf-1 gene in the samples was analyzed by methylation specific (MSP) PCR. RESULTS: The expression of Apaf-1 mRNA in gastric cancer tissue samples was 51%. The LOH frequency of D12S346, D12S1706, D12S327, D12S1657 and D12S393 was 33%, 8%, 58%, 12% and 42%, respectively. Fifty percent LOH was found at two sites and 17% LOH at three sites. Apaf-1 mRNA expression decreased significantly in 13 cases (rs = 0.487, P = 0.003). The rate of Apaf-1 promoter methylation was 49% in gastric cancer tissue samples and 23% in para-cancerous tissue samples. Promoter methylation occurred significantly in 16 of 18 gastric cancer tissue samples with decreased expression of Apaf-1 mRNA rs = 0.886, P = 10-6). CONCLUSION: The expression of Apaf-1 gene is low in gastric cancer tissues. Methylation of Apaf-1 gene promoter and LOH in domain of 12q22-23 are the main reasons for the expression and altered expression of Apaf-1 gene.
基金Supported by Liaoning Provincial Science and Technology Key Project for Translational Medicine,No.2014225020Outstanding Scientific Fund of Shengjing Hospital,No.201102Liaoning Provincial Science and Technology Key Project for Translational Medicine,No.2016509
文摘AIM To investigate whether M1 or M2 polarization contributes to the therapeutic effects of mesenchymal stem cells(MSCs) in acute hepatic failure(AHF).METHODS MSCs were transfused into rats with AHF induced by D-galactosamine(DGal N). The therapeutic effects of MSCs were evaluated based on survival rate and hepatocyte proliferation and apoptosis. Hepatocyte regeneration capacity was evaluated by the expression of the hepatic progenitor surface marker epithelial cell adhesion molecule(Ep CAM). Macrophage polarization was analyzed by M1 markers [CD68,tumor necrosis factor alpha(TNF-α),interferon-γ(IFN-γ),inducible nitric oxide synthase(INOS)] and M2 markers [CD163,interleukin(IL)-4,IL-10,arginase-1(Arg-1)] in the survival and death groups after MSC transplantation.RESULTS The survival rate in the MSC-treated group was increased compared with the DPBS-treated control group(37.5% vs 10%). MSC treatment protected rats with AHF by reducing apoptotic hepatocytes and promoting hepatocyte regeneration. Immunohistochemical analysis showed that MSC treatment significantly increased the expression of Ep CAM compared with the control groups(P < 0.001). Expression of Ep CAM in the survival group was significantly up-regulated compared with the death group after MSC transplantation(P = 0.003). Transplantation of MSCs significantly improved the expression of CD163 and increased the gene expression of IL-10 and Arg-1 in the survival group. IL-4 concentrations were significantly increased compared to the death group after MSC transplantation(88.51 ± 24.51 pg/m L vs 34.61 ± 6.6 pg/m L,P < 0.001). In contrast,macrophages showed strong expression of CD68,TNF-α,and INOS in the death group. The concentration of IFN-γ was significantly increased compared to the survival group after MSC transplantation(542.11 ± 51.59 pg/m L vs 104.07 ± 42.80 pg/m L,P < 0.001).CONCLUSION M2 polarization contributes to the therapeutic effects of MSCs in AHF by altering levels of anti-inflammatory and pro-inflammatory factors.
基金This study was supported by the National Natural Science Foundation of China (51502054)the Postdoctoral Science Foundation of China (2014M551236)the Postdoctoral Science Foundation of Heilongjiang Province (LBH-Z14083).
文摘Bismuth ferrite(BiFeO3)-based materials are multiferroic materials widely studied.This study reports that strong ferroelectricity and enhanced magnetic performance are simultaneously obtained in the quenched(1−x)BiFeO3−xBaTiO3(BFBT100x,x=0.2 and 0.3)ceramics.Quenching treatment can reduce the amount of defects and Fe2+ions and make the defect dipole in a random state,which is conducive to improving the ferroelectricity and magnetism.Compared with the conventional sintered samples,the quenched ceramics have higher remnant and saturation polarization.As for magnetism,the coercive field(Hc)of the quenched ceramics is smaller and the quenching treatment can increase the maximum magnetization by up to 15%.
基金supported by the National Natural Science Foundation of China(51502054)the Postdoctoral Science Foundation of China(2014M551236)the Postdoctoral Science Foundation of Heilongjiang Province(LBH-Z14083).
文摘Multiferroic BiFeO_(3)-based ceramics were synthesized using the rapid liquid-phase sintering method.The rare-earth ion(Sm^(3+),Gd^(3+),Y^(3+))doping causes structural distortion without changing the intrinsic rhombohedral perovskite structure.Raman analysis shows that the effect of doping on E modes is greater than A1 modes,and the microstructure of FeO_(6) octahedron can be regulated by ion doping.A-site trivalent ion doped ceramics exhibit improved magnetism compared with pure BiFeO_(3) ceramic,which originated from the suppressed spiral spin structure of Fe ions.The tilt of FeO_(6) octahedron as a typical structure instability causes the anomalous change of the imaginary part of permittivity at high frequency,and doped ceramics exhibit natural resonance around 16-17 GHz.
基金This work was supported by the National Natural Science Foundation of China(U2130110 and 51502054).
文摘The barium ferrite BaTi_(x)Fe_(12−x)O_(19)(x=0.2,0.4,0.6,0.8)(BFTO-x)ceramics doped by Ti4+were synthesized by a modified sol–gel method.The crystal structure and magnetic structure of the samples were determined by neutron diffraction,and confirm that the BFTO-x ceramics were high quality single phase with sheet microstructure.With x increasing from 0.2 to 0.8,the saturation magnetization(M_(s))decreases gradually but the change trend of coercivity(H_(c))is complex under the synergy of the changed grain size and the magnetic crystal anisotropy field.Relying on the high valence of Ti^(4+),double resonance peaks are obtained in the curves of the imaginary part of magnetic conductivity(μ′′)and the resonance peaks could move toward the low frequency with the increase of x,which facilitate the samples perform an excellent wideband modulation microwave absorption property.In the x=0.2 sample,the maximum reflection loss(RL)can reach−44.9 dB at the thickness of only 1.8 mm,and the bandwidth could reach 5.28 GHz at 2 mm when RL is less than−10 dB.All the BFTO-x ceramics show excellent frequency modulation ability varying from 18(x=0.8)to 4 GHz(x=0.4),which covers 81%of the investigated frequency in microwave absorption field.This work not only implements the tunable of electromagnetic parameters but also broadens the application of high-performance microwave absorption devices.
