There is an increasing demand for salmonid authentication due to the globalization of the salmonid trade.DNA barcoding and mini-DNA barcoding are widely used for identifying fish species based on a fragment of the mit...There is an increasing demand for salmonid authentication due to the globalization of the salmonid trade.DNA barcoding and mini-DNA barcoding are widely used for identifying fish species based on a fragment of the mitochondrial cytochrome c oxidase subunit I(COI)sequence.In this study,rainbow trout(Oncorhynchus mykiss),steelhead trout(O.mykiss),and Atlantic salmon(Salmo salar)collected from two salmonid aquaculture bases in China were authenticated by DNA barcoding(about 650 bp)and mini-DNA barcoding(127 bp)to evaluate the accuracy of the two methods in the identification of different salmonid species.The results revealed that both methods could effectively distinguish O.mykiss and S.salar with 100%accuracy.However,the two methods failed to separate rainbow trout(O.mykiss)and steelhead trout(O.mykiss),which are the same species but cultured in different water environments.Moreover,salmonid samples from three main distribution channels in the Qingdao area(traditional supermarkets,online supermarkets,and sushi bars)were identified by the two methods.Substitution of S.salar with O.mykiss was discovered,and the 27.78%overall substitution rate of salmonids in the Qingdao area was higher than those in other regions reported in previous studies.In addition,the mislabeling rates of salmonids from traditional supermarkets,online supermarkets,and sushi bars were compared in this study.The mislabeling rate was significantly greater in sushi bars(50%)than in the other two channels(16.67%),suggesting that stronger monitoring and enforcement measures are necessary for the aquatic food catering industry.展开更多
目的:探讨口服磷酸钠盐导致的急性磷酸盐肾病(APN)患者的临床病理特征及预后。方法:回顾分析2010年1月至2022年11月国家肾脏疾病临床医学研究中心行肾活检诊断为APN的患者,分析其临床表现、病理特点、治疗及预后。结果:共纳入APN患者9例...目的:探讨口服磷酸钠盐导致的急性磷酸盐肾病(APN)患者的临床病理特征及预后。方法:回顾分析2010年1月至2022年11月国家肾脏疾病临床医学研究中心行肾活检诊断为APN的患者,分析其临床表现、病理特点、治疗及预后。结果:共纳入APN患者9例,其中男性7例,肾活检时年龄60.1±9.1岁。患者均因需行结肠镜检查口服磷酸钠盐,服药前血清肌酐(SCr)水平均在正常范围,1例无高危因素,其余8例存在2~4种高危因素(中位3种)。诊断急性肾损伤(AKI)时,SCr为257.24±63.65μmol/L;除1例血磷升高,余患者血钙、血磷水平均在正常范围。尿检无红细胞,仅2例出现少量蛋白尿。肾活检光镜下主要表现为肾小管间质损伤,肾小管上皮细胞、管腔和间质中均观察到多处偏振光无折光性的嗜碱性钙盐沉积(全片共25~58处)。6例患者接受泼尼松(15~30 mg/d)治疗。中位随访14月,7例患者肾功能改善,但SCr均未恢复至正常范围,末次随访SCr为175.92±45.05μmol/L,估算的肾小球滤过率(e GFR)为36.33±12.83 m L/(min·1.73m^(2))。结论:口服磷酸钠盐导致的APN通常起病较为隐匿,肾活检有助于明确诊断。对于存在高危因素的患者应慎用磷酸盐洗肠液,并密切监测肾功能变化,以提早识别和处理。展开更多
基金the National Key Research and Development Program of China(No.2019YFD0901000)the Natural Science Foundation of Shandong Pro-vince,China(No.ZR2020MC194).
文摘There is an increasing demand for salmonid authentication due to the globalization of the salmonid trade.DNA barcoding and mini-DNA barcoding are widely used for identifying fish species based on a fragment of the mitochondrial cytochrome c oxidase subunit I(COI)sequence.In this study,rainbow trout(Oncorhynchus mykiss),steelhead trout(O.mykiss),and Atlantic salmon(Salmo salar)collected from two salmonid aquaculture bases in China were authenticated by DNA barcoding(about 650 bp)and mini-DNA barcoding(127 bp)to evaluate the accuracy of the two methods in the identification of different salmonid species.The results revealed that both methods could effectively distinguish O.mykiss and S.salar with 100%accuracy.However,the two methods failed to separate rainbow trout(O.mykiss)and steelhead trout(O.mykiss),which are the same species but cultured in different water environments.Moreover,salmonid samples from three main distribution channels in the Qingdao area(traditional supermarkets,online supermarkets,and sushi bars)were identified by the two methods.Substitution of S.salar with O.mykiss was discovered,and the 27.78%overall substitution rate of salmonids in the Qingdao area was higher than those in other regions reported in previous studies.In addition,the mislabeling rates of salmonids from traditional supermarkets,online supermarkets,and sushi bars were compared in this study.The mislabeling rate was significantly greater in sushi bars(50%)than in the other two channels(16.67%),suggesting that stronger monitoring and enforcement measures are necessary for the aquatic food catering industry.
文摘目的:探讨口服磷酸钠盐导致的急性磷酸盐肾病(APN)患者的临床病理特征及预后。方法:回顾分析2010年1月至2022年11月国家肾脏疾病临床医学研究中心行肾活检诊断为APN的患者,分析其临床表现、病理特点、治疗及预后。结果:共纳入APN患者9例,其中男性7例,肾活检时年龄60.1±9.1岁。患者均因需行结肠镜检查口服磷酸钠盐,服药前血清肌酐(SCr)水平均在正常范围,1例无高危因素,其余8例存在2~4种高危因素(中位3种)。诊断急性肾损伤(AKI)时,SCr为257.24±63.65μmol/L;除1例血磷升高,余患者血钙、血磷水平均在正常范围。尿检无红细胞,仅2例出现少量蛋白尿。肾活检光镜下主要表现为肾小管间质损伤,肾小管上皮细胞、管腔和间质中均观察到多处偏振光无折光性的嗜碱性钙盐沉积(全片共25~58处)。6例患者接受泼尼松(15~30 mg/d)治疗。中位随访14月,7例患者肾功能改善,但SCr均未恢复至正常范围,末次随访SCr为175.92±45.05μmol/L,估算的肾小球滤过率(e GFR)为36.33±12.83 m L/(min·1.73m^(2))。结论:口服磷酸钠盐导致的APN通常起病较为隐匿,肾活检有助于明确诊断。对于存在高危因素的患者应慎用磷酸盐洗肠液,并密切监测肾功能变化,以提早识别和处理。
