本试验旨在通过RNA-seq技术在转录组水平分析早期蛋白质限制与后期补偿对羔羊抗氧化性能的影响。试验选取15日龄断母乳湖羊公羔32只,随机分为两组,一组饲喂正常蛋白水平的代乳粉(CP为25%)和开食料(CP为21%),记为对照组(normal protein l...本试验旨在通过RNA-seq技术在转录组水平分析早期蛋白质限制与后期补偿对羔羊抗氧化性能的影响。试验选取15日龄断母乳湖羊公羔32只,随机分为两组,一组饲喂正常蛋白水平的代乳粉(CP为25%)和开食料(CP为21%),记为对照组(normal protein level,NPL);另一组饲喂蛋白水平分别为19%的代乳粉和15%的开食料,记为试验组(low protein level,LPL)。人工饲喂相应的代乳粉至60日龄,开食料自由采食。61-90 d所有试验羊均自由采食正常蛋白水平(CP为21%)的开食料。分别在羔羊60和90日龄,每组随机选取2只羔羊进行屠宰,采用RNA-seq技术鉴定蛋白质限制与补偿条件下肝脏组织差异表达基因(DEGs),通过生物信息学分析,筛选与抗氧化功能密切相关的通路,利用qRT-PCR的方法验证抗氧化酶系基因表达水平。低蛋白水平日粮引起羔羊肝脏组织差异表达基因有302个,其中23个差异表达基因参与机体抗氧化调节过程,且表达水平呈显著下调。GO富集分析发现差异表达基因参与氧化还原酶活性(GO:0016491)、谷胱甘肽转移酶活性(GO:0004364)和氧化还原过程(GO:0055114)。KEGG分析发现,抗氧化相关基因主要富集到P450介导的外源性物质代谢和谷胱甘肽代谢通路;细胞因子受体作用通路相关基因表达水平显著提高。恢复蛋白水平30 d后,抗氧化相关基因表达水平与对照组无差异。日粮蛋白质水平与机体抗氧化功能密切相关。蛋白质限制导致肝脏基因表达发生改变,降低了抗氧化相关基因的表达水平,DEGs可通过外源性物质代谢、谷胱甘肽代谢、细胞因子受体作用通路等信号通路影响肝脏抗氧化和免疫功能。展开更多
伪随机数发生器(Pseudorandom Number Generator,PRNG)是密码学应用系统的一个重要组成部分.本文利用线性同余发生器(Linear Congruential Generator,LCG)、流密码算法RC4和密码学Hash函数,构造了一种基于软件实现的组合式伪随机数发生...伪随机数发生器(Pseudorandom Number Generator,PRNG)是密码学应用系统的一个重要组成部分.本文利用线性同余发生器(Linear Congruential Generator,LCG)、流密码算法RC4和密码学Hash函数,构造了一种基于软件实现的组合式伪随机数发生器.该伪随机数发生器可以快速生成伪随机数列并且从理论上证明了产生的数列具备不可预测性.同时,采用美国国家标准和技术研究院(National Institute of Standard Technology,NIST)发行的随机性测试包对该新构造的伪随机数发生器所产生的序列进行统计测试.实验结果显示,该新构造的伪随机数发生器产生的序列能够很好地通过各项测试,可以应用于信息安全领域.展开更多
To investigate the effects of allicin on chickens＇ lipid and antioxidant performance, Hy-laying hens＇ diets were replenished with 0 mg · kg-1, 50 mg · kg-1, 100 mg · kg-1, and 150 mg · kg-1 allic...To investigate the effects of allicin on chickens＇ lipid and antioxidant performance, Hy-laying hens＇ diets were replenished with 0 mg · kg-1, 50 mg · kg-1, 100 mg · kg-1, and 150 mg · kg-1 allicin for 42 days, respectively. The alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, triglyceride（TG）, total cholesterol（TCHO）, high density lipoprotein（HDL）, and low density lipoprotein（LDL） levels were measured in chicken serum. Superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px） activity and malondialdehyde（MDA） levels were measured in chicken serum and liver tissue homogenate. The results showed that the supplement dose of allicin tested did not significantly change the activity of ALT or AST（P〉0.05）; TG and CHOL levels decreased with the increase of allicin additive doses, and the difference between treatment groups and CG was significant（P〈0.05）, and there was the best effect with 100 mg · kg-1; allicin significantly reduced the content of MDA, and increased SOD and GSH-Px activities compared with CG（P〈0.05）, and 100 mg · kg-1 of allicin resulted in the strongest SOD and GSH-Px activity. The antioxidant function test results of liver tissue homogenate were consistant with that of serum. Our findings indicated that allicin could enhance antioxidant capacity and reduce blood lipid level in chickens and 100 mg · kg-1 was the optimal amount of allicin additives.展开更多
文摘本试验旨在通过RNA-seq技术在转录组水平分析早期蛋白质限制与后期补偿对羔羊抗氧化性能的影响。试验选取15日龄断母乳湖羊公羔32只,随机分为两组,一组饲喂正常蛋白水平的代乳粉(CP为25%)和开食料(CP为21%),记为对照组(normal protein level,NPL);另一组饲喂蛋白水平分别为19%的代乳粉和15%的开食料,记为试验组(low protein level,LPL)。人工饲喂相应的代乳粉至60日龄,开食料自由采食。61-90 d所有试验羊均自由采食正常蛋白水平(CP为21%)的开食料。分别在羔羊60和90日龄,每组随机选取2只羔羊进行屠宰,采用RNA-seq技术鉴定蛋白质限制与补偿条件下肝脏组织差异表达基因(DEGs),通过生物信息学分析,筛选与抗氧化功能密切相关的通路,利用qRT-PCR的方法验证抗氧化酶系基因表达水平。低蛋白水平日粮引起羔羊肝脏组织差异表达基因有302个,其中23个差异表达基因参与机体抗氧化调节过程,且表达水平呈显著下调。GO富集分析发现差异表达基因参与氧化还原酶活性(GO:0016491)、谷胱甘肽转移酶活性(GO:0004364)和氧化还原过程(GO:0055114)。KEGG分析发现,抗氧化相关基因主要富集到P450介导的外源性物质代谢和谷胱甘肽代谢通路;细胞因子受体作用通路相关基因表达水平显著提高。恢复蛋白水平30 d后,抗氧化相关基因表达水平与对照组无差异。日粮蛋白质水平与机体抗氧化功能密切相关。蛋白质限制导致肝脏基因表达发生改变,降低了抗氧化相关基因的表达水平,DEGs可通过外源性物质代谢、谷胱甘肽代谢、细胞因子受体作用通路等信号通路影响肝脏抗氧化和免疫功能。
文摘伪随机数发生器(Pseudorandom Number Generator,PRNG)是密码学应用系统的一个重要组成部分.本文利用线性同余发生器(Linear Congruential Generator,LCG)、流密码算法RC4和密码学Hash函数,构造了一种基于软件实现的组合式伪随机数发生器.该伪随机数发生器可以快速生成伪随机数列并且从理论上证明了产生的数列具备不可预测性.同时,采用美国国家标准和技术研究院(National Institute of Standard Technology,NIST)发行的随机性测试包对该新构造的伪随机数发生器所产生的序列进行统计测试.实验结果显示,该新构造的伪随机数发生器产生的序列能够很好地通过各项测试,可以应用于信息安全领域.
文摘To investigate the effects of allicin on chickens＇ lipid and antioxidant performance, Hy-laying hens＇ diets were replenished with 0 mg · kg-1, 50 mg · kg-1, 100 mg · kg-1, and 150 mg · kg-1 allicin for 42 days, respectively. The alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, triglyceride（TG）, total cholesterol（TCHO）, high density lipoprotein（HDL）, and low density lipoprotein（LDL） levels were measured in chicken serum. Superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px） activity and malondialdehyde（MDA） levels were measured in chicken serum and liver tissue homogenate. The results showed that the supplement dose of allicin tested did not significantly change the activity of ALT or AST（P〉0.05）; TG and CHOL levels decreased with the increase of allicin additive doses, and the difference between treatment groups and CG was significant（P〈0.05）, and there was the best effect with 100 mg · kg-1; allicin significantly reduced the content of MDA, and increased SOD and GSH-Px activities compared with CG（P〈0.05）, and 100 mg · kg-1 of allicin resulted in the strongest SOD and GSH-Px activity. The antioxidant function test results of liver tissue homogenate were consistant with that of serum. Our findings indicated that allicin could enhance antioxidant capacity and reduce blood lipid level in chickens and 100 mg · kg-1 was the optimal amount of allicin additives.
