Colocalization of Neurotensin and Corticotropin-Releasing Factor in Oval Nucleus of the Bed Nuclei of Stria Terminalis in the Rat

Ju et al first parcellated in detail the bed nuclei of stria terminalis according to its cyto-and chemoarchitecture.They found that the oval nucleus(OV)was rich in neurotensin(NT)-and corticotropin-releasing factor(CRF)like immunoreactivities.Using indirect immunohistochemical technique it has been shown in the present study that both neuropeptides show quite similar distribution pattern in OV of the Sprague-Dawley rat(180-250 g).

The Descending Projection of Serotoninergic,Fibers in the Raphe Nucleus of Brainstem and Its,Adjacent Areas in the Rat——A Double Labeled Study of HRP and Immunohistochemistry

Rats(Sprague Dawley,200-300g)wereoperated under anesthesia with nembuta1.Afterthe laminectomy,20-25%aqueous solution ofHRP(Sigma 6)was injected unilatera11y intothe spinal cord at cervical or lumbar leve1.Theanimals were allowed to survive for 36-48hours and transcardically perfused with 0.9%NaCl,3.5%paraforma1dehyde,and 10%sucr-ose in 0.1mol/L PB.The brain was removed andimmersed in 10%sucrose at 4℃.