OBJECTIVE Eriocalyxin B(EriB)is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora,traditional Chinese herbal medicines,possesses strong antileukemic activity with low toxicity.In murine t(8;21)leukemia m...OBJECTIVE Eriocalyxin B(EriB)is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora,traditional Chinese herbal medicines,possesses strong antileukemic activity with low toxicity.In murine t(8;21)leukemia models,EriB remarkably prolong the survival time and decreased the xenograft tumor size by targeting AML1-ETO oncoprotein.In angiogenesis research by the highly vascularized chorioallantoic membrane(CAM)of the chicken embryo further confirm its antiangiogenic activity.Microarray offers a high efficient approach to study the gene expression profile treated by EriB,so as to provide systematic information about potential mechanisms of EriB curing AML.METHODS The t(8;21)AML cell line Kasumi-1 is most sensitive to EriB.Cells are treated with Eri-B(0.5μmol·L-1)and collected in 2,6,12 and 24h,respectively.Using human cDNA microarray,we investigate the changes of differential gene expression of Kasumi-1cells by time before and after Eri-B treatment.Meanwhile,the mRNA expression of TRAF2,DEDD2,BAG3,SAT1,IQGAP1,C-myc and GRB2 is detected by semi-quantitative RT-PCR and real-time quantitative PCR.RESULTS The genes regulated significantly are correlated with cell proliferation,apoptosis,cell circle,regulation of transcription,response to stimulies and metabolism.Regulation of TNFR mediated apoptotic signaling by EriB plays a key role to induce apoptosis and cell circle,involved NF-κB,Ras-MAPK,cAMP/PKA,PI3K/Akt,Cyt c/caspase and p53-Rb signal pathways.After detected by SqRT-PCR and real-time quantitative PCR,the mRNA expressions of BAG3,DEDD2,SAT1 and C-myc are significantly changed.CONCLUSION These findings describes the probable mechanisms involved and the value of EriB as a promising candidate targeting apoptosis cascade and cell circle in treatment of AML.展开更多
Abstract: In our search for bioactive compounds from the whole plant of Picria felterrae Lour., three new phenylethanoid glycosides, picfeosides A-C (1-3), along with five known phenylethanoid glycosides, namely wiede...Abstract: In our search for bioactive compounds from the whole plant of Picria felterrae Lour., three new phenylethanoid glycosides, picfeosides A-C (1-3), along with five known phenylethanoid glycosides, namely wiedemannioside (4), acteoside (5), acteoside isomer (6), cis-acteoside isomer (7), and cis-acteoside (8), were isolated using several chromatographic purification steps, including semipreparation HPLC on RP-18. The structures of the new compounds were elucidated on the basis of extensive spectroscopic analysis.展开更多
基金The project supported by National Natural Science Foundation of China(30772637)Yunnan Provincial Science and Technology Department(2014IA033)
文摘OBJECTIVE Eriocalyxin B(EriB)is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora,traditional Chinese herbal medicines,possesses strong antileukemic activity with low toxicity.In murine t(8;21)leukemia models,EriB remarkably prolong the survival time and decreased the xenograft tumor size by targeting AML1-ETO oncoprotein.In angiogenesis research by the highly vascularized chorioallantoic membrane(CAM)of the chicken embryo further confirm its antiangiogenic activity.Microarray offers a high efficient approach to study the gene expression profile treated by EriB,so as to provide systematic information about potential mechanisms of EriB curing AML.METHODS The t(8;21)AML cell line Kasumi-1 is most sensitive to EriB.Cells are treated with Eri-B(0.5μmol·L-1)and collected in 2,6,12 and 24h,respectively.Using human cDNA microarray,we investigate the changes of differential gene expression of Kasumi-1cells by time before and after Eri-B treatment.Meanwhile,the mRNA expression of TRAF2,DEDD2,BAG3,SAT1,IQGAP1,C-myc and GRB2 is detected by semi-quantitative RT-PCR and real-time quantitative PCR.RESULTS The genes regulated significantly are correlated with cell proliferation,apoptosis,cell circle,regulation of transcription,response to stimulies and metabolism.Regulation of TNFR mediated apoptotic signaling by EriB plays a key role to induce apoptosis and cell circle,involved NF-κB,Ras-MAPK,cAMP/PKA,PI3K/Akt,Cyt c/caspase and p53-Rb signal pathways.After detected by SqRT-PCR and real-time quantitative PCR,the mRNA expressions of BAG3,DEDD2,SAT1 and C-myc are significantly changed.CONCLUSION These findings describes the probable mechanisms involved and the value of EriB as a promising candidate targeting apoptosis cascade and cell circle in treatment of AML.
文摘Abstract: In our search for bioactive compounds from the whole plant of Picria felterrae Lour., three new phenylethanoid glycosides, picfeosides A-C (1-3), along with five known phenylethanoid glycosides, namely wiedemannioside (4), acteoside (5), acteoside isomer (6), cis-acteoside isomer (7), and cis-acteoside (8), were isolated using several chromatographic purification steps, including semipreparation HPLC on RP-18. The structures of the new compounds were elucidated on the basis of extensive spectroscopic analysis.
