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Optimization of extraction of phenolics from leaves of Ficus virens 被引量:3
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作者 Xiao-xin CHEN Xiao-bing WU +4 位作者 Wei-ming CHAI Hui-ling FENG Yan SHI han-tao zhou Qing-xi CHEN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2013年第10期903-915,共13页
In this research,the conditions for extraction of phenolics from leaves of Ficus virens were optimized using response surface methodology(RSM).The extraction abilities of phenolics(EAP) and flavonoids(EAF),the 2,2-dip... In this research,the conditions for extraction of phenolics from leaves of Ficus virens were optimized using response surface methodology(RSM).The extraction abilities of phenolics(EAP) and flavonoids(EAF),the 2,2-diphenyl-1-pierylhydrazyl(DPPH) free-radical scavenging potential,and the ferric reducing/antioxidant power(FRAP) were used as quality indicators.The results of single-factor experiments showed that temperature,ethanol concentration,extraction time,and the number of extraction cycles were the main influencing variables,and these provided key information for the central composite design.The results of RSM fitted well to a second degree polynomial model and more than 98% of the variability was explained.The ideal extraction conditions for EAP,EAF,DPPH free-radical scavenging potential,and FRAP were obtained.Considering the four quality indicators overall,the ideal extraction conditions were 58% ethanol at 57 °C for 37 min with three extraction cycles.At the ideal extraction conditions,the values of EAP,EAF,DPPH free-radical scavenging potential,and FRAP were 5.72%,3.09%,58.88 mg ascorbic acid equivalent(AAE)/g dry weight(DW),and 15.86 mg AAE/g DW,respectively.In addition,linear correlations were observed between EAP,EAF,and antioxidant potential. 展开更多
关键词 Ficus virens Phenolics FLAVONOIDS ANTIOXIDANTS Response surface methodology(RSM)
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