Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcripti...Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.展开更多
High-temperature infrared(IR)camouflage is crucial to the effective concealment of high-temperature objects but remains a challenging issue,as the thermal radiation of an object is proportional to the fourth power of ...High-temperature infrared(IR)camouflage is crucial to the effective concealment of high-temperature objects but remains a challenging issue,as the thermal radiation of an object is proportional to the fourth power of temperature(T4).Here,we experimentally demonstrate high-temperature IR camouflage with efficient thermal management.By combining a silica aerogel for thermal insulation and a Ge/ZnS multilayer wavelength-selective emitter for simultaneous radiative cooling(high emittance in the 5-8μm non-atmospheric window)and IR camouflage(low emittance in the 8-14μm atmospheric window),the surface temperature of an object is reduced from 873 to 410 K.The IR camouflage is demonstrated by indoor/outdoor(with/without earthshine)radiation temperatures of 310/248 K for an object at 873/623 K and a 78% reduction in with-earthshine lock-on range.This scheme may introduce opportunities for high-temperature thermal management and infrared signal processing.展开更多
基金supported by the National Key Program on Transgenic Research of China(No.2018ZX08021001-005-001)the National Natural Science Foundation of China(Nos.31672479,31701496,and 31801429)+1 种基金the Major Program of Shandong Province Natural Science Foundation(No.ZR2018ZB0213)the Assisted Project by Heilong Jiang Postdoctoral Funds for Scientific Research Initiation(LBH-Z18262).
文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants.
基金supported by the National Key Research and Development Program of China(2017YFA0205700 and 2017YFE0100200)the National Natural Science Foundation of China(Grant Nos.61975181,61775194,and 61950410608).
文摘High-temperature infrared(IR)camouflage is crucial to the effective concealment of high-temperature objects but remains a challenging issue,as the thermal radiation of an object is proportional to the fourth power of temperature(T4).Here,we experimentally demonstrate high-temperature IR camouflage with efficient thermal management.By combining a silica aerogel for thermal insulation and a Ge/ZnS multilayer wavelength-selective emitter for simultaneous radiative cooling(high emittance in the 5-8μm non-atmospheric window)and IR camouflage(low emittance in the 8-14μm atmospheric window),the surface temperature of an object is reduced from 873 to 410 K.The IR camouflage is demonstrated by indoor/outdoor(with/without earthshine)radiation temperatures of 310/248 K for an object at 873/623 K and a 78% reduction in with-earthshine lock-on range.This scheme may introduce opportunities for high-temperature thermal management and infrared signal processing.
