Active ulcerative colitis (UC) is associated with elevated granulocytes and mo nocytes/macrophages (GM) which show activation behavior and increased survival t ime. Further, fecal calprotectin (a stable neutrophil pro...Active ulcerative colitis (UC) is associated with elevated granulocytes and mo nocytes/macrophages (GM) which show activation behavior and increased survival t ime. Further, fecal calprotectin (a stable neutrophil protein) level parallels i ntestinal inflammation and can predict UC relapse. Since GM are major sources of inflammatory cytokines and chemokines, they are suspected to have roles in the initiation and perpetuation of UC. Our objective was to investigated relationshi ps between peripheral blood (PB) neutrophils, calprotectin, and UC disease activ ity. Full PB and calprotectin were determined in 69 healthy controls and 31 pati ents with UC, then 7 randomly selected patients received GM adsorptive apheresis (GMA) with Adacolumn, 10 sessions of 60-min duration each. Patients with UC ha d higher neutrophil counts (P < 0.001), but lower lymphocyte counts (P < 0.001) compared with controls. Further, fecal calprotectin levels showed a correlation with UC clinical activity index (CAI; P < 0.001) and mucosal inflammation (P < 0 .001) . Following GMA, there were falls in neutrophils (P < 0.02), CAI (P < 0.02 ) and calprotectin (P < 0.02). In conclusion, GM appear to contribute to intesti nal inflammation and UC activity and reduction of these cells by GMA should bene fit patients with active UC. Further, the correlations among calprotectin, UC ac tivities, and PB neutrophils should serve as the basis for preemptive actions to control this disease.展开更多
文摘Active ulcerative colitis (UC) is associated with elevated granulocytes and mo nocytes/macrophages (GM) which show activation behavior and increased survival t ime. Further, fecal calprotectin (a stable neutrophil protein) level parallels i ntestinal inflammation and can predict UC relapse. Since GM are major sources of inflammatory cytokines and chemokines, they are suspected to have roles in the initiation and perpetuation of UC. Our objective was to investigated relationshi ps between peripheral blood (PB) neutrophils, calprotectin, and UC disease activ ity. Full PB and calprotectin were determined in 69 healthy controls and 31 pati ents with UC, then 7 randomly selected patients received GM adsorptive apheresis (GMA) with Adacolumn, 10 sessions of 60-min duration each. Patients with UC ha d higher neutrophil counts (P < 0.001), but lower lymphocyte counts (P < 0.001) compared with controls. Further, fecal calprotectin levels showed a correlation with UC clinical activity index (CAI; P < 0.001) and mucosal inflammation (P < 0 .001) . Following GMA, there were falls in neutrophils (P < 0.02), CAI (P < 0.02 ) and calprotectin (P < 0.02). In conclusion, GM appear to contribute to intesti nal inflammation and UC activity and reduction of these cells by GMA should bene fit patients with active UC. Further, the correlations among calprotectin, UC ac tivities, and PB neutrophils should serve as the basis for preemptive actions to control this disease.
