Dear editor,We have read with great interest the recent article“Comparison of different versions of the quick sequential organ failure assessment for predicting in-hospital mortality of sepsis patients:A retrospectiv...Dear editor,We have read with great interest the recent article“Comparison of different versions of the quick sequential organ failure assessment for predicting in-hospital mortality of sepsis patients:A retrospective observational study”.[1]They showed that the performance of the procalcitonin-enhanced qSOFA(PqSOFA)was similar to that of the qSOFA,and the lactate-enhanced qSOFA(LqSOFA)and the modified qSOFA(MqSOFA)were superior to the qSOFA for predicting in-hospital mortality.Further,they suggested that the MqSOFA should be a candidate for the qSOFA in the emergency department(ED).We congratulate the authors for their contributions.However,we has several concerns about this research.展开更多
The study of unit commitment (UC) aims to find reasonable schedules for generators to optimize power systems’ operation. Many papers have been published that solve UC through different methods. Articles that systemat...The study of unit commitment (UC) aims to find reasonable schedules for generators to optimize power systems’ operation. Many papers have been published that solve UC through different methods. Articles that systematically summarize UC problems’ progress in order to update researchers interested in this field are needed. Because of its promising performance, stochastic programming (SP) has become increasingly researched. Most papers, however, present SP’s UC solving approaches differently, which masks their relationships and makes it hard for new researchers to quickly obtain a general idea. Therefore, this paper tries to give a structured bibliographic survey of SP’s applications in UC problems.展开更多
Long-term and stable preservation of bacteriophages is of crucial importance.Although many efforts have been made in the past decades to explore the influence of external factors on bacteriophage preservation,there is...Long-term and stable preservation of bacteriophages is of crucial importance.Although many efforts have been made in the past decades to explore the influence of external factors on bacteriophage preservation,there is still little understanding,and a systematic description is lacking.In this study,we explored the influence of different factors on the preservation of lytic bacteriophage VP3,one of the typing bacteriophages of Vibrio cholerae O1 biotype El Tor,and attempted to optimize its preservation.We examined external factors,including temperature,solution,and cryoprotectant,in stable cooling/freezing conditions or alternate cooling/freezing and thawing.We found that whether in Luria-Bertani(LB)medium or SM buffer,in terms of 20-week stable cooling or freezing,−20℃ was the most damaging while 4℃,−80℃,and−196℃ were protective.Thirteen cycles of alternate cooling/freezing and thawing caused a loss in the survival rates of bacteriophages.The addition of cryoprotectant,glycerol(30%,w/v)or dimethyl sulfoxide(DMSO,10%,w/v)significantly improved the survival rates of bacteriophages preserved at−20℃.However,at 4℃,−80℃,and−196℃,the cryoprotectant effect was only slightly positive or even harmful.In summary,for bacteriophage VP3,the best preservation method is to directly preserve the bacteriophage stocks in LB medium at−80℃ or−196℃ instead of storing them in SM buffer or adding cryoprotectant.Our results provided insights into the external influencing factors on bacteriophage VP3 during preservation at low temperature and can be applied to the optimization of bacteriophage preservation in the future.展开更多
This paper aims to provide an efficient and straightforward structural form-finding method for designers to extrapolate component forms during the conceptual stage.The core idea is to optimize the classical method of ...This paper aims to provide an efficient and straightforward structural form-finding method for designers to extrapolate component forms during the conceptual stage.The core idea is to optimize the classical method of structural form-finding based on principal stress lines by using parametric tools.The traditional operating process of this method relies excessively on the designer’s engineering experience and lacks precision.Meanwhile,the current optimization work for this method is overly complicated for architects,and limitations in component type and final result exist.Therefore,to facilitate an architect’s conceptual work,the optimization metrics of the method in this paper are set as simplicity,practicality,freedom,and rapid feedback.For that reason,this paper optimizes the method from three aspects:modeling strategy for continuum structures,classification processing of data by using the k-nearest neighbor algorithm,and topological form-finding process based on stress lines.Eventually,it allows architects to create structural texture with formal aesthetics and modify it in real time on the basis of structural analysis results.This paper also explores a comprehensive application strategy with internal force analysis diagramming to form-finding.The finite element analysis tool Karamba3D verifies the structural performance of the form-finding method.The performance is compared with that of the conventional form,and the comparison results show the practicality and potential of the strategy in this paper.展开更多
文摘Dear editor,We have read with great interest the recent article“Comparison of different versions of the quick sequential organ failure assessment for predicting in-hospital mortality of sepsis patients:A retrospective observational study”.[1]They showed that the performance of the procalcitonin-enhanced qSOFA(PqSOFA)was similar to that of the qSOFA,and the lactate-enhanced qSOFA(LqSOFA)and the modified qSOFA(MqSOFA)were superior to the qSOFA for predicting in-hospital mortality.Further,they suggested that the MqSOFA should be a candidate for the qSOFA in the emergency department(ED).We congratulate the authors for their contributions.However,we has several concerns about this research.
文摘The study of unit commitment (UC) aims to find reasonable schedules for generators to optimize power systems’ operation. Many papers have been published that solve UC through different methods. Articles that systematically summarize UC problems’ progress in order to update researchers interested in this field are needed. Because of its promising performance, stochastic programming (SP) has become increasingly researched. Most papers, however, present SP’s UC solving approaches differently, which masks their relationships and makes it hard for new researchers to quickly obtain a general idea. Therefore, this paper tries to give a structured bibliographic survey of SP’s applications in UC problems.
基金National Science and Technology Major Project on Important Infectious Diseases Prevention and Control(2018ZX10734404)National Science and Technology Infrastructure of China(National Pathogen Resource Center‐NPRC‐32)+1 种基金National Science and Technology Fundamental Resources Investigation Program of China(2021FY100904)National Nature Science Foundation of China(82161148010‐4,52076140).
文摘Long-term and stable preservation of bacteriophages is of crucial importance.Although many efforts have been made in the past decades to explore the influence of external factors on bacteriophage preservation,there is still little understanding,and a systematic description is lacking.In this study,we explored the influence of different factors on the preservation of lytic bacteriophage VP3,one of the typing bacteriophages of Vibrio cholerae O1 biotype El Tor,and attempted to optimize its preservation.We examined external factors,including temperature,solution,and cryoprotectant,in stable cooling/freezing conditions or alternate cooling/freezing and thawing.We found that whether in Luria-Bertani(LB)medium or SM buffer,in terms of 20-week stable cooling or freezing,−20℃ was the most damaging while 4℃,−80℃,and−196℃ were protective.Thirteen cycles of alternate cooling/freezing and thawing caused a loss in the survival rates of bacteriophages.The addition of cryoprotectant,glycerol(30%,w/v)or dimethyl sulfoxide(DMSO,10%,w/v)significantly improved the survival rates of bacteriophages preserved at−20℃.However,at 4℃,−80℃,and−196℃,the cryoprotectant effect was only slightly positive or even harmful.In summary,for bacteriophage VP3,the best preservation method is to directly preserve the bacteriophage stocks in LB medium at−80℃ or−196℃ instead of storing them in SM buffer or adding cryoprotectant.Our results provided insights into the external influencing factors on bacteriophage VP3 during preservation at low temperature and can be applied to the optimization of bacteriophage preservation in the future.
文摘This paper aims to provide an efficient and straightforward structural form-finding method for designers to extrapolate component forms during the conceptual stage.The core idea is to optimize the classical method of structural form-finding based on principal stress lines by using parametric tools.The traditional operating process of this method relies excessively on the designer’s engineering experience and lacks precision.Meanwhile,the current optimization work for this method is overly complicated for architects,and limitations in component type and final result exist.Therefore,to facilitate an architect’s conceptual work,the optimization metrics of the method in this paper are set as simplicity,practicality,freedom,and rapid feedback.For that reason,this paper optimizes the method from three aspects:modeling strategy for continuum structures,classification processing of data by using the k-nearest neighbor algorithm,and topological form-finding process based on stress lines.Eventually,it allows architects to create structural texture with formal aesthetics and modify it in real time on the basis of structural analysis results.This paper also explores a comprehensive application strategy with internal force analysis diagramming to form-finding.The finite element analysis tool Karamba3D verifies the structural performance of the form-finding method.The performance is compared with that of the conventional form,and the comparison results show the practicality and potential of the strategy in this paper.
