江苏省钢铁行业公路运输车辆污染减排分析

江苏省既是交通大省,又是钢铁大省。基于公路运输及钢铁行业统计数据,本文分析江苏省公路货运量、重型货车保有量、钢材产量等,并结合钢铁行业超低排放改造现状,研究清洁运输部分超低排放改造情况,测算公路运输部分在重型货车排放标准升级后的污染减排潜力。结果显示,江苏省货物运输以公路运输为主,清洁运输方式比例较低,钢铁行业完成清洁运输部分超低排放改造后,交通运输结构显著优化,重型货车排放标准升级带来的大气污染物减排幅度明显,环境效益可观。当前,应加快推进钢铁行业超低排放改造,同时为其他行业实施升级改造提供参考。

微创经皮锁定加压钢板内固定术治疗四肢骨折的临床疗效探讨

目的探讨四肢骨折患者采用微创经皮锁定加压钢板内固定术治疗的临床效果。方法对该院收治的四肢骨折患者展开研究,共计选取80例患者为研究对象,病例收集时间在2019年1月—2020年12月,所有患者均拟行手术治疗,按照均等单盲方将80例患者分为对照组(n=40)和观察组(n=40),两组均给予钢板内固定术进行治疗,而观察组在此基础上实施微创经皮锁定加压钢板内固定术进行治疗,对比两组患者的临床治疗效果。结果观察组手术时长、住院时长和骨折愈合时间均短于对照组,手术失血量少于对照组,差异有统计学意义(P<0.05);观察组手术前疼痛评分与对照组相比差异无统计学意义(P>0.05);观察组手术后3 d和手术后1周疼痛评分均低于对照组,差异有统计学意义(P<0.05)。观察组手术前白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、超敏C反应蛋白(hs-CRP)和肿瘤坏死因子-α(TNF-α)水平与对照组相比差异无统计学意义(P>0.05);观察组手术后IL-6、IL-8、hs-CRP和TNF-α水平均低于对照组,差异有统计学意义(P<0.05)。观察组手术前躯体功能、心理功能、社会功能和物质生活状态4个维度生活质量评分与对照组相比差异无统计学意义(P>0.05);观察组手术后生活质量4个维度评分均高于对照组,差异有统计学意义(P<0.05)。观察组并发症发生率(7.50%)低于对照组(25.00%),差异有统计学意义(χ^(2)=4.501,P<0.05)。结论四肢骨折患者采用微创经皮锁定加压钢板内固定术治疗具有创伤小,并发症少的优势,可以有效促进患者尽早康复,提高患者的生活质量,临床治疗效果显著。

Nrf2-mediated ferroptosis of spermatogenic cells involved in male reproductive toxicity induced by polystyrene nanoplastics in mice

Microplastics(MPs)and nanoplastics(NPs)have become hazardous materials due to the massive amount of plastic waste and disposable masks,but their specific health effects remain uncertain.In this study,fluorescence-labeled polystyrene NPs(PS-NPs)were injected into the circulatory systems of mice to determine the distribution and potential toxic effects of NPs in vivo.Interestingly,whole-body imaging found that PS-NPs accumulated in the testes of mice.Therefore,the toxic effects of PS-NPs on the reproduction systems and the spermatocytes cell line of male mice,and their mechanisms,were investigated.After oral exposure to PS-NPs,their spermatogenesis was affected and the spermatogenic cells were damaged.The spermatocyte cell line GC-2 was exposed to PS-NPs and analyzed using RNA sequencing(RNA-seq)to determine the toxic mechanisms;a ferroptosis pathway was found after PS-NP exposure.The phenomena and indicators of ferroptosis were then determined and verified by ferroptosis inhibitor ferrostatin-1(Fer-1),and it was also found that nuclear factor erythroid 2-related factor 2(Nrf2)played an important role in spermatogenic cell ferroptosis induced by PS-NPs.Finally,it was confirmed in vivo that this mechanism of Nrf2 played a protective role in PS-NPs-induced male reproductive toxicity.This study demonstrated that PS-NPs induce male reproductive dysfunction in mice by causing spermatogenic cell ferroptosis dependent on Nrf2.

A proteomic analysis of Bcl-2 regulation of cell cycle arrest:insight into the mechanisms

B cell lymphoma 2(Bcl-2)is an important antiapoptotic gene that plays a dual role in the maintenance of the dynamic balance between the survival and death of cancer cells.In our previous study,Bcl-2 was shown to delay the G0/G1 to S phase entry by regulating the mitochondrial metabolic pathways to produce lower levels of adenosine triphosphate(ATP)and reactive oxygen species(ROS).However,the detailed molecular mechanisms or pathways by which Bcl-2 regulates the cell cycle remain unknown.Here,we compared the effects of Bcl-2 overexpression with an empty vector control in the NIH3T3 cell line synchronized by serum starvation,and evaluated the effects using proteomic analysis.The effect of Bcl-2 on cell cycle regulation was detected by monitoring Bcl-2 and p27 expression.The result of subsequent proteomic analysis of Bcl-2 overexpressing cells identified 169 upregulated and 120 downregulated proteins with a 1.5-fold change.These differentially expressed proteins were enriched in a number of signaling pathways predominantly involving the ribosome and oxidative phosphorylation,according to the data of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses.These results indicated that Bcl-2 potentially acts at the translation level to influence proteins or enzymes of the respiratory chain or in the ribosome,and thereby regulates the cell cycle.Additionally,differentially expressed proteins involved in oxidative phosphorylation were determined to account for most of the effects of Bcl-2 on the cell cycle mediated by the mitochondrial pathway investigated in our previous study.These results can provide assistance for additional in-depth studies on the regulation of the cell cycle by Bcl-2.The results of the proteomic analysis determined the mechanism of Bcl-2-dependent delay of the cell cycle progression.In summary,the results of this study provide a novel mechanistic basis for identifying the key proteins or pathways for designing and developing precisely targeted cancer drugs.

A novel anticancer property of Lycium barbarum polysaccharide in triggering ferroptosis of breast cancer cells

Breast cancer is one of the most malignant tumors and is associated with high mortality rates among women.Lycium barbarum polysaccharide(LBP)is an extract from the fruits of the traditional Chinese herb,L.barbarum.LBP is a promising anticancer drug,due to its high activity and low toxicity.Although it has anticancer properties,its mechanisms of action have not been fully established.Ferroptosis,which is a novel anticancer strategy,is a cell death mechanism that relies on iron-dependent lipid reactive oxygen species(ROS)accumulation.In this study,human breast cancer cells(Michigan Cancer Foundation-7(MCF-7)and MD Anderson-Metastatic Breast-231(MDA-MB-231))were treated with LBP.LBP inhibited their viability and proliferation in association with high levels of ferroptosis.Therefore,we aimed to ascertain whether LBP reduced cell viability through ferroptosis.We found that the structure and function of mitochondria,lipid peroxidation,and expression of solute carrier family 7 member 11(SLC7 A11,also known as x CT,the light-chain subunit of cystine/glutamate antiporter system X_(c)^(-))and glutathione peroxidase 4(GPX4)were altered by LBP.Moreover,the ferroptosis inhibitor,Ferrostatin-1(Fer-1),rescued LBP-induced ferroptosis-associated events including reduced cell viability and glutathione(GSH)production,accumulation of intracellular free divalent iron ions and malondialdehyde(MDA),and down-regulation of the expression of x CT and GPX4.Erastin(x CT inhibitor)and RSL3(GPX4 inhibitor)inhibited the expression of x CT and GPX4,respectively,which was lower after the co-treatment of LBP with Erastin and RSL3.These results suggest that LBP effectively prevents breast cancer cell proliferation and promotes ferroptosis via the x CT/GPX4 pathway.Therefore,LBP exhibits novel anticancer properties by triggering ferroptosis,and may be a potential therapeutic option for breast cancer.

基于Mo_(0.8)Si_(0.2)纳米线的中红外超导单光子探测器

由于中红外波段光子能量大大低于可见/近红外波段光子,中红外单光子探测是光电探测领域的研究热点和难点.本文研制出一种低能隙超导体MoSi纳米线中红外单光子探测器.该探测器具有极高的灵敏度,最长响应波长超过10μm,在1.55~5.07μm波段上的量子效率均高于97%,噪声等效功率低至10^(-18)W/Hz^(1/2)量级.该工作在前沿科学研究和军事国防领域具有重要应用前景.

Saturation efficiency for detecting 1550 nm photons with a 2×2 array of Mo0.8Si0.2 nanowires at 2.2 K

Amorphous materials are attractive candidates for fabricating the superconducting nanowire single-photon detectors(SNSPDs) due to their superior tolerance and scalability over crystalline niobium nitride. However, the reduced superconducting transition temperature degenerates both operating temperature and saturation efficiency. Herein, the SNSPD(6.5 nm thickness and 50 nm width) based on the amorphous Mo0.8Si0.2 film with a high optical absorption coefficient demonstrates close-to-unity intrinsic detection efficiency for 1550 nm photons from 75 m K to 2.2 K. Further, a high-performance array SNSPD with optimized 90 nm-width wires is also demonstrated. As-fabricated uniform 4-pixel SNSPD exhibits a saturation plateau for the photon counts at 2.2 K,which overcomes the limitation of operation at low temperature(< 1 K) for traditional amorphous SNSPDs.Coupled with superior intrinsic quantum efficiency, highly efficient photon counts, and low dark count ratio, this detector paves a way for achieving high efficiency and superior yield for large array systems.