Studies on pattern formation in coculture cell systems can provide insights into many physiological and pathological processes.Here,we investigate how the extracellular matrix(ECM)may influence the patterning in cocul...Studies on pattern formation in coculture cell systems can provide insights into many physiological and pathological processes.Here,we investigate how the extracellular matrix(ECM)may influence the patterning in coculture systems.The model coculture system we use is composed of highly motile invasive breast cancer cells,initially mixed with inert nonmetastatic cells on a 2D substrate and covered with a Matrigel layer introduced to mimic ECM.We observe that the invasive cells exhibit persistent centripetal motion and yield abnormal aggregation,rather than random spreading,due to a“collective pulling”effect resulting from ECM-mediated transmission of active contractile forces generated by the polarized migration of the invasive cells along the vertical direction.The mechanism we report may open a new window for the understanding of biological processes that involve multiple types of cells.展开更多
Studiesonpattern formation in coculturecell systemscan provide insights intomany physiological and pathologicalprocesses Here,we investigate how theextracellular matrix(ECM)may influence the patterning in coculture sy...Studiesonpattern formation in coculturecell systemscan provide insights intomany physiological and pathologicalprocesses Here,we investigate how theextracellular matrix(ECM)may influence the patterning in coculture systems.Themodel coculture system we use is composed of highly motile invasivebreast cancer cells,initially mixed with inert nonmetastatic cells on a 2D substrate and covered with a Matrigel layer introduced to mimic ECM.We observe that the invasive cells exhibit persistent centripetal motion and yield abnormal aggregation,rather than random spreading,due to a“cllective pulling”effect resultingfrom ECM-mediated transmssion of active contractile forces generated bythe polarized migration of the invasive cells along the vertical direction.Themechanism we report may open a new window for the understanding of biological processes that involve multiple types of cells.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.11774394,11974066,12074407,and 12090054)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDB33000000)+2 种基金the Start-Up Fund and University Graduate Fellowship of Arizona State University,the Natural Science Foundation of Chongqing Municipal Science and Technology Commission(Grant No.cstc2019jcyj-msxmX0477)the Fundamental Research Funds for the Central Universities(Grant No.2019CDYGYB007)the Capital Health Development Research Project,China(Grant No.2020-2-2072).
文摘Studies on pattern formation in coculture cell systems can provide insights into many physiological and pathological processes.Here,we investigate how the extracellular matrix(ECM)may influence the patterning in coculture systems.The model coculture system we use is composed of highly motile invasive breast cancer cells,initially mixed with inert nonmetastatic cells on a 2D substrate and covered with a Matrigel layer introduced to mimic ECM.We observe that the invasive cells exhibit persistent centripetal motion and yield abnormal aggregation,rather than random spreading,due to a“collective pulling”effect resulting from ECM-mediated transmission of active contractile forces generated by the polarized migration of the invasive cells along the vertical direction.The mechanism we report may open a new window for the understanding of biological processes that involve multiple types of cells.
基金supported by the National Naturai Science Foundation of China(Grant Nos.11774394,11974066,12074407,and 12090054)the Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDB330000)+2 种基金the Start-Up Fund and University Graduate Fllowship of Arizona State University,the Natural Science Foundation of Chongqing Municipal Science and Technology Commission(Grant No.cstc2019jcyj-msxmX0477)the Fundamental Research Funds for the Central Universities(Grant No.2019CDYGYB007)and the Capital Health Development Research Project,China(Grant No.2020-2-2072).
文摘Studiesonpattern formation in coculturecell systemscan provide insights intomany physiological and pathologicalprocesses Here,we investigate how theextracellular matrix(ECM)may influence the patterning in coculture systems.Themodel coculture system we use is composed of highly motile invasivebreast cancer cells,initially mixed with inert nonmetastatic cells on a 2D substrate and covered with a Matrigel layer introduced to mimic ECM.We observe that the invasive cells exhibit persistent centripetal motion and yield abnormal aggregation,rather than random spreading,due to a“cllective pulling”effect resultingfrom ECM-mediated transmssion of active contractile forces generated bythe polarized migration of the invasive cells along the vertical direction.Themechanism we report may open a new window for the understanding of biological processes that involve multiple types of cells.
