Oct4 is a key component of the pluripotency regulatory network,and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells(ESCs)or their differentiati...Oct4 is a key component of the pluripotency regulatory network,and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells(ESCs)or their differentiation into trophoblast.Oct4 of maternal origin is postulated to play critical role in defining totipotency and inducing pluripotency during embryonic development.However,the genetic elimination of maternal Oct4 using a Cre-lox approach in mouse revealed that the establishment of totipotency in maternal Oct4–depleted embryos was not affected,and that these embryos could complete full-term development without any obvious defect.These results indicate that Oct4 is not essential for the initiation of pluripotency,in contrast to its critical role in maintaining pluripotency.This conclusion is further supported by the formation of Oct4-GFP–and Nanog-expressing inner cell masses(ICMs)in embryos with complete inactivation of both maternal and zygotic Oct4 expression and the reprogramming of fibroblasts into fully pluripotent cells by Oct4-deficient oocytes.展开更多
基金This research was supported by the Max Planck Society,DFG grant SI 1695/1-2(SPP1356)NIH grant R01HD059946-01 from the Eunice Kennedy Shriver National Institute of Child Health&Human Development.We thank Areti Malapetsas for final editing。
文摘Oct4 is a key component of the pluripotency regulatory network,and its reciprocal interaction with Cdx2 has been shown to be a determinant of either the self-renewal of embryonic stem cells(ESCs)or their differentiation into trophoblast.Oct4 of maternal origin is postulated to play critical role in defining totipotency and inducing pluripotency during embryonic development.However,the genetic elimination of maternal Oct4 using a Cre-lox approach in mouse revealed that the establishment of totipotency in maternal Oct4–depleted embryos was not affected,and that these embryos could complete full-term development without any obvious defect.These results indicate that Oct4 is not essential for the initiation of pluripotency,in contrast to its critical role in maintaining pluripotency.This conclusion is further supported by the formation of Oct4-GFP–and Nanog-expressing inner cell masses(ICMs)in embryos with complete inactivation of both maternal and zygotic Oct4 expression and the reprogramming of fibroblasts into fully pluripotent cells by Oct4-deficient oocytes.
