Optimization of media constituents for the production of lipase in solid state fermentation by Yarrowia lipolytica from palm Kernal cake (Elaeis guineensis)

The production of extra cellular lipase in Solid State Fermentation (SSF) using Yarrowia lipolytica NCIM 3589 with Palm Kernal cake (Elaeis guineensis) has been studied. Different parameters such as incubation time, inoculum level, initial moisture content, carbon level and nitrogen level of the medium were optimized. Screening of various process variables has been accomplished with the help of Plackett-Burman design. The maximum lipase activity of 18.58 units per gram of dry fermented substrate (U/gds) was observed with the substrate of Palm Kernal cake in four days of fermentation.

Growth rate data fitting of Yarrowia lipolytica NCIM 3589 using logistic equation and artificial neural networks

Growth rate of Yarrowia lipolytica NCIM 3589 was observed in a fermentation medium consisting of peptone, yeast extract, sodium chloride. Logistic equation was fitted to the growth data (time vs. biomass concentration) and compared with the prediction given by Artificial Neural Networks (ANN). ANN was found to be superior in describing growth characteristics. A single MATLAB programme is developed to fit the growth data by logistic equation and ANN.