World Health Organization has estimated that annually 24.3%(13.7 million of 56.1 million)of all global premature deaths and28.1%(1.57 million of 5.60 million)deaths among children under five,are linked to environmenta...World Health Organization has estimated that annually 24.3%(13.7 million of 56.1 million)of all global premature deaths and28.1%(1.57 million of 5.60 million)deaths among children under five,are linked to environmental factors[1].展开更多
The increasing need to monitor emergent pollutants,such as persistent organic pollutants,endocrine disruptors,and antibiotics,is driven by their impacts on environment and health.1,2 Nevertheless,instrumental methods ...The increasing need to monitor emergent pollutants,such as persistent organic pollutants,endocrine disruptors,and antibiotics,is driven by their impacts on environment and health.1,2 Nevertheless,instrumental methods based on mass spectrometry are intricate,take a considerable amount of time,and necessitate costly equipment,posing challenges for their application in field monitoring.The development of biosensors has offered a prompt,sensitive,selective,and real-time technique for the identification and monitoring of pollutants.A bioreceptor,a transducer,and a signal processor compose a biosensor,which transforms the recognition of target into detectable signals.3 The performance of biosensors relies heavily on the bioreceptor as its binding affinity and specificity are vital determinants.The most frequently used biosensors are developed with antibodies as bioreceptors that detect environmental pollutants effectively.This is due to their high specificity and binding affinity to analytes,which results from antigen−antibody immune interactions.However,screening antibodies with high specificity and affinity can be a challenging process in biosensor development for diverse pollutants.Furthermore,these antibodies may not always maintain a constant recognition performance in a harsh environmental matrix.Therefore,development of new bioreceptors with a rapid screening method that are reliable and cost-effective is essential for improving the convenience in on-site monitoring of environmental pollutants.展开更多
Mercury is a global pollutant due to its widespread use,emission,and long-range transport(Blum,2013;Pacyna et al.,2010).It is considered a priority pollutant due to its neurological toxicity,persistence,and bioaccum...Mercury is a global pollutant due to its widespread use,emission,and long-range transport(Blum,2013;Pacyna et al.,2010).It is considered a priority pollutant due to its neurological toxicity,persistence,and bioaccumulation(Pacyna et al.,2010;Sharma et al.,2015).Mercury pollution can occur when products that contain mercury are improperly disposed of and mercury is released into the air,water,and soil(Zhang and Wong,2007).An estimated 22%of the annual world usage of mercury is in electrical equipment such as batteries,thermometers,and discharge lamps,and electronic devices such as monitors and展开更多
The occurrence of a large number of diverse arsenic species in the environment and in biological systems makes it important to compare their relative toxicity. The toxicity of arsenic species has been examined in vari...The occurrence of a large number of diverse arsenic species in the environment and in biological systems makes it important to compare their relative toxicity. The toxicity of arsenic species has been examined in various cell lines using different assays, making comparison difficult. We report real-time cell sensing of two human cell lines to examine the cytotoxicity of fourteen arsenic species: arsenite(As~Ⅲ), monomethylarsonous acid(MMA~Ⅲ) originating from the oxide and iodide forms, dimethylarsinous acid(DMA~Ⅲ), dimethylarsinic glutathione(DMAG~Ⅲ), phenylarsine oxide(PAO~Ⅲ), arsenate(AsV), monomethylarsonic acid(MMA~Ⅴ), dimethylarsinic acid(DMA~Ⅴ),monomethyltrithioarsonate(MMTTA~Ⅴ), dimethylmonothioarsinate(DMMTA~Ⅴ),dimethyldithioarsinate(DMDTA~Ⅴ), 3-nitro-4-hydroxyphenylarsonic acid(Roxarsone, Rox),and 4-aminobenzenearsenic acid(p-arsanilic acid, p-ASA). Cellular responses were measured in real time for 72 hr in human lung(A549) and bladder(T24) cells. IC50 values for the arsenicals were determined continuously over the exposure time, giving rise to IC50 histograms and unique cell response profiles. Arsenic accumulation and speciation were analyzed using inductively coupled plasma-mass spectrometry(ICP-MS). On the basis of the 24-hr IC50 values, the relative cytotoxicity of the tested arsenicals was in the following decreasing order: PAO~Ⅲ? MMA~Ⅲ≥ DMA~Ⅲ≥ DMAG~Ⅲ≈ DMMTA~Ⅴ≥ As~Ⅲ? MMTTA~Ⅴ〉 AsV〉 DMDTA~Ⅴ〉DMA~Ⅴ〉 MMA~Ⅴ≥ Rox ≥ p-ASA. Stepwise shapes of cell response profiles for DMA~Ⅲ, DMAG~Ⅲ,and DMMTA~Ⅴcoincided with the conversion of these arsenicals to the less toxic pentavalent DMA~Ⅴ. Dynamic monitoring of real-time cellular responses to fourteen arsenicals provided useful information for comparison of their relative cytotoxicity.展开更多
Hundreds of millions of people around the world are exposed to elevated concentrations of inorganic and organic arsenic compounds, increasing the risk of a wide range of health effects. Studies of the environmental fa...Hundreds of millions of people around the world are exposed to elevated concentrations of inorganic and organic arsenic compounds, increasing the risk of a wide range of health effects. Studies of the environmental fate and human health effects of arsenic require authentic arsenic compounds. We summarize here the synthesis and characterization of more than a dozen methylated and thiolated arsenic compounds that are not commercially available. We discuss the methods of synthesis for the following14 trivalent(Ⅲ) and pentavalent() arsenic compounds: monomethylarsonous acid(MMA~Ⅲ), dicysteinylmethyldithioarsenite(MMA~Ⅲ(Cys)_2), monomethylarsonic acid(MMA~Ⅴ),monomethylmonothioarsonic acid(MMMTAⅤ) or monothio-MMA~Ⅴ, monomethyldithioarsonic acid(MMDTA~Ⅴ) or dithio-MMA~Ⅴ, monomethyltrithioarsonate(MMTTA~Ⅴ) or trithio-MMA~Ⅴ,dimethylarsinous acid(DMA~Ⅲ), dimethylarsino-glutathione(DMA~Ⅲ(SG)), dimethylarsinic acid(DMA~Ⅴ), dimethylmonothioarsinic acid(DMMTA~Ⅴ) or monothio-DMAⅤ, dimethyldithioarsinic acid(DMDTA~Ⅴ) or dithio-DMA~Ⅴ, trimethylarsine oxide(TMAO~Ⅴ), arsenobetaine(AsB), and an arsenicin-A model compound. We have reviewed and compared the available methods,synthesized the arsenic compounds in our laboratories, and provided characterization information. On the basis of reaction yield, ease of synthesis and purification of product, safety considerations, and our experience, we recommend a method for the synthesis of each of these arsenic compounds.展开更多
Arsenic(As)and antimony(Sb)rank 1 st and 244 th,respectively,on the 2019 Substance Priority List of the Agency for Toxic Substances and Disease Registry(ATSDR,2019).ATSDR lists substances,in order of priority,that are...Arsenic(As)and antimony(Sb)rank 1 st and 244 th,respectively,on the 2019 Substance Priority List of the Agency for Toxic Substances and Disease Registry(ATSDR,2019).ATSDR lists substances,in order of priority,that are"most commonly found at facilities on the National Priorities List(NPL)and which are determined to pose the most significant potential threat to human health due to their known or suspected toxicity and potential for human exposure at these NPL sites".展开更多
基金supported by the National Natural Science Foundation of China(22136006,22021003)the K.C.Wong Education Foundation of China(GJTD-2020-03)。
文摘World Health Organization has estimated that annually 24.3%(13.7 million of 56.1 million)of all global premature deaths and28.1%(1.57 million of 5.60 million)deaths among children under five,are linked to environmental factors[1].
基金supported by National Natural Science Fund for Excellent Young Scientists Fund Program(Overseas)the National Natural Science Foundation of China(Grant no.22276199).
文摘The increasing need to monitor emergent pollutants,such as persistent organic pollutants,endocrine disruptors,and antibiotics,is driven by their impacts on environment and health.1,2 Nevertheless,instrumental methods based on mass spectrometry are intricate,take a considerable amount of time,and necessitate costly equipment,posing challenges for their application in field monitoring.The development of biosensors has offered a prompt,sensitive,selective,and real-time technique for the identification and monitoring of pollutants.A bioreceptor,a transducer,and a signal processor compose a biosensor,which transforms the recognition of target into detectable signals.3 The performance of biosensors relies heavily on the bioreceptor as its binding affinity and specificity are vital determinants.The most frequently used biosensors are developed with antibodies as bioreceptors that detect environmental pollutants effectively.This is due to their high specificity and binding affinity to analytes,which results from antigen−antibody immune interactions.However,screening antibodies with high specificity and affinity can be a challenging process in biosensor development for diverse pollutants.Furthermore,these antibodies may not always maintain a constant recognition performance in a harsh environmental matrix.Therefore,development of new bioreceptors with a rapid screening method that are reliable and cost-effective is essential for improving the convenience in on-site monitoring of environmental pollutants.
基金Alberta Health,Alberta Innovates,the Canada Research Chairs Programthe Canadian Institutes of Health Researchthe Natural Sciences and Engineering Research Council of Canada for their support
文摘Mercury is a global pollutant due to its widespread use,emission,and long-range transport(Blum,2013;Pacyna et al.,2010).It is considered a priority pollutant due to its neurological toxicity,persistence,and bioaccumulation(Pacyna et al.,2010;Sharma et al.,2015).Mercury pollution can occur when products that contain mercury are improperly disposed of and mercury is released into the air,water,and soil(Zhang and Wong,2007).An estimated 22%of the annual world usage of mercury is in electrical equipment such as batteries,thermometers,and discharge lamps,and electronic devices such as monitors and
基金supported by Alberta Health, Alberta Innovates, the Canada Research Chairs Programthe Canadian Institutes of Health Research (CIHR)the Natural Sciences and Engineering Research Council (NSERC) of Canada
文摘The occurrence of a large number of diverse arsenic species in the environment and in biological systems makes it important to compare their relative toxicity. The toxicity of arsenic species has been examined in various cell lines using different assays, making comparison difficult. We report real-time cell sensing of two human cell lines to examine the cytotoxicity of fourteen arsenic species: arsenite(As~Ⅲ), monomethylarsonous acid(MMA~Ⅲ) originating from the oxide and iodide forms, dimethylarsinous acid(DMA~Ⅲ), dimethylarsinic glutathione(DMAG~Ⅲ), phenylarsine oxide(PAO~Ⅲ), arsenate(AsV), monomethylarsonic acid(MMA~Ⅴ), dimethylarsinic acid(DMA~Ⅴ),monomethyltrithioarsonate(MMTTA~Ⅴ), dimethylmonothioarsinate(DMMTA~Ⅴ),dimethyldithioarsinate(DMDTA~Ⅴ), 3-nitro-4-hydroxyphenylarsonic acid(Roxarsone, Rox),and 4-aminobenzenearsenic acid(p-arsanilic acid, p-ASA). Cellular responses were measured in real time for 72 hr in human lung(A549) and bladder(T24) cells. IC50 values for the arsenicals were determined continuously over the exposure time, giving rise to IC50 histograms and unique cell response profiles. Arsenic accumulation and speciation were analyzed using inductively coupled plasma-mass spectrometry(ICP-MS). On the basis of the 24-hr IC50 values, the relative cytotoxicity of the tested arsenicals was in the following decreasing order: PAO~Ⅲ? MMA~Ⅲ≥ DMA~Ⅲ≥ DMAG~Ⅲ≈ DMMTA~Ⅴ≥ As~Ⅲ? MMTTA~Ⅴ〉 AsV〉 DMDTA~Ⅴ〉DMA~Ⅴ〉 MMA~Ⅴ≥ Rox ≥ p-ASA. Stepwise shapes of cell response profiles for DMA~Ⅲ, DMAG~Ⅲ,and DMMTA~Ⅴcoincided with the conversion of these arsenicals to the less toxic pentavalent DMA~Ⅴ. Dynamic monitoring of real-time cellular responses to fourteen arsenicals provided useful information for comparison of their relative cytotoxicity.
基金supported by Alberta Health, Alberta Innovates, the Canada Research Chairs Programthe Canadian Institutes of Health Research (CIHR)the Natural Sciences and Engineering Research Council (NSERC) of Canada
文摘Hundreds of millions of people around the world are exposed to elevated concentrations of inorganic and organic arsenic compounds, increasing the risk of a wide range of health effects. Studies of the environmental fate and human health effects of arsenic require authentic arsenic compounds. We summarize here the synthesis and characterization of more than a dozen methylated and thiolated arsenic compounds that are not commercially available. We discuss the methods of synthesis for the following14 trivalent(Ⅲ) and pentavalent() arsenic compounds: monomethylarsonous acid(MMA~Ⅲ), dicysteinylmethyldithioarsenite(MMA~Ⅲ(Cys)_2), monomethylarsonic acid(MMA~Ⅴ),monomethylmonothioarsonic acid(MMMTAⅤ) or monothio-MMA~Ⅴ, monomethyldithioarsonic acid(MMDTA~Ⅴ) or dithio-MMA~Ⅴ, monomethyltrithioarsonate(MMTTA~Ⅴ) or trithio-MMA~Ⅴ,dimethylarsinous acid(DMA~Ⅲ), dimethylarsino-glutathione(DMA~Ⅲ(SG)), dimethylarsinic acid(DMA~Ⅴ), dimethylmonothioarsinic acid(DMMTA~Ⅴ) or monothio-DMAⅤ, dimethyldithioarsinic acid(DMDTA~Ⅴ) or dithio-DMA~Ⅴ, trimethylarsine oxide(TMAO~Ⅴ), arsenobetaine(AsB), and an arsenicin-A model compound. We have reviewed and compared the available methods,synthesized the arsenic compounds in our laboratories, and provided characterization information. On the basis of reaction yield, ease of synthesis and purification of product, safety considerations, and our experience, we recommend a method for the synthesis of each of these arsenic compounds.
文摘Arsenic(As)and antimony(Sb)rank 1 st and 244 th,respectively,on the 2019 Substance Priority List of the Agency for Toxic Substances and Disease Registry(ATSDR,2019).ATSDR lists substances,in order of priority,that are"most commonly found at facilities on the National Priorities List(NPL)and which are determined to pose the most significant potential threat to human health due to their known or suspected toxicity and potential for human exposure at these NPL sites".