A real-time fluorescent quantitative immuno-polymerase chain reaction(RT-IPCR)assay was developed for the detection of non-dioxinlike polychlorinated biphenyl(PCB)congener in soil samples.Based on the construction of ...A real-time fluorescent quantitative immuno-polymerase chain reaction(RT-IPCR)assay was developed for the detection of non-dioxinlike polychlorinated biphenyl(PCB)congener in soil samples.Based on the construction of 3,4-dichlorinated biphenyl(IUPAC PCB12)hapten and its immunogen,the specific polyclonal antibodies(pAbs)to PCB12 was obtained and used to develop a direct competitive RT-IPCR assay.Using the optimized assay,a standard curve for PCB12 was prepared.The linear range for the determination of PCB12 was from 10.0to 1.0 × 106fg/mL with a correlation coefficient of 0.98 and a detection limit of 1.53fg/mL.The RT-IPCR assays were tested for their cross-reactivity profiles using four selected congeners and four Aroclor products.The results for the soil samples correlated with the concentrations of PCBs obtained by gas chromatography/mass spectrometry.This highly specific,sensitive,and robust assay can be applied to on-site tests of PCBs and serve as a model for other pollutant immunoassays.展开更多
Gastric cancer(GC)is the fifth most common cancer among all cancer fields and currently the third most common cause of cancer-related deaths both in men and women worldwide.Additionally,the incidence and mortality of ...Gastric cancer(GC)is the fifth most common cancer among all cancer fields and currently the third most common cause of cancer-related deaths both in men and women worldwide.Additionally,the incidence and mortality of GC significantly varies due to different eating habits and environmental and genetic factors in different areas.Remarkably,with the development of techniques and surgical equipment along with newly emerging drugs and therapeutics,the 5-year survival for GC patients has increased and the death rate has decreased.In this perspective,based on current therapies in GC field around the world,we present a systematic discussion about the state of advanced clinical treatments including surgical resection,systemic chemotherapy,targeted therapy,radiotherapy and enhanced recovery after surgery.Although challenges remain which require further study and investigation,in this review,we mainly focus on the promising clinical treatment strategies in GC to date.展开更多
基金supported by the National Natural Science Foundation of China (No. 21177082)the Science and Technology Commission of Shanghai Municipality in China (Key Project of Fundamental Research,No.09JC1407600)+1 种基金the Shanghai Jiao Tong University science and technology innovation special fund development projectsthe Postdoctoral Science Foundation of Shanghai (No. 11R21414600)
文摘A real-time fluorescent quantitative immuno-polymerase chain reaction(RT-IPCR)assay was developed for the detection of non-dioxinlike polychlorinated biphenyl(PCB)congener in soil samples.Based on the construction of 3,4-dichlorinated biphenyl(IUPAC PCB12)hapten and its immunogen,the specific polyclonal antibodies(pAbs)to PCB12 was obtained and used to develop a direct competitive RT-IPCR assay.Using the optimized assay,a standard curve for PCB12 was prepared.The linear range for the determination of PCB12 was from 10.0to 1.0 × 106fg/mL with a correlation coefficient of 0.98 and a detection limit of 1.53fg/mL.The RT-IPCR assays were tested for their cross-reactivity profiles using four selected congeners and four Aroclor products.The results for the soil samples correlated with the concentrations of PCBs obtained by gas chromatography/mass spectrometry.This highly specific,sensitive,and robust assay can be applied to on-site tests of PCBs and serve as a model for other pollutant immunoassays.
文摘Gastric cancer(GC)is the fifth most common cancer among all cancer fields and currently the third most common cause of cancer-related deaths both in men and women worldwide.Additionally,the incidence and mortality of GC significantly varies due to different eating habits and environmental and genetic factors in different areas.Remarkably,with the development of techniques and surgical equipment along with newly emerging drugs and therapeutics,the 5-year survival for GC patients has increased and the death rate has decreased.In this perspective,based on current therapies in GC field around the world,we present a systematic discussion about the state of advanced clinical treatments including surgical resection,systemic chemotherapy,targeted therapy,radiotherapy and enhanced recovery after surgery.Although challenges remain which require further study and investigation,in this review,we mainly focus on the promising clinical treatment strategies in GC to date.
