目的建立应用血栓弹力图(TEG)及凝血指标综合评估上消化道出血患者凝血功能的输血结局预测模型。方法从输血(科)管理系统和医院信息系统(HIS)系统收集浙江省人民医院及其淳安分院消化内科2018年6月~2021年6月收治的101名符合临床诊断标...目的建立应用血栓弹力图(TEG)及凝血指标综合评估上消化道出血患者凝血功能的输血结局预测模型。方法从输血(科)管理系统和医院信息系统(HIS)系统收集浙江省人民医院及其淳安分院消化内科2018年6月~2021年6月收治的101名符合临床诊断标准的上消化道出血患者,根据结局是否输血分为输血组(n=56)和未输血组(n=45),以及按照肝硬化与否分为肝硬化组(n=74)和非肝硬化组(n=27),同时收集40名非上消化道出血患者的阴性对照组。对比各组的TEG检测R、K、α、MA参数,凝血功能检测PT、INR、APTT、TT、Fib,血常规检测Hb、Plt、WBC、NEUT%,以及生化检测Alb、SCr、ALT、AST、GGT等指标(值);分析TEG指标与传统凝血功能指标的相关性;采用单因素和多因素分析,筛选输血相关因素建立预测模型。结果输血组与未输血组比较:TEG的K(min)为3.86±3.12 vs 2.50±1.47,α(°)为54.00±14.08 vs 61.05±10.88,MA(mm)为51.12±13.37 vs 58.26±11.08(P<0.01);凝血指标检测PT(s)为16.36±7.45 vs 13.44±1.50,Fib(g)为1.59±0.87 vs 2.35±1.09(P<0.01);血常规检测NEUT%为0.75±0.13 vs 0.66±0.15,Hb(g/L)为68.04±14.49 vs 100.73±22.92(P<0.01);生化检测Alb(g/L)为29.73±6.08 vs 33.73±7.19,SCr(nmol/L)为99.50±53.55 vs 76.25±19.28(P<0.01)。相关性分析:APTT与R、K值呈正相关,与α、MA值呈负相关;Fib与K值呈负相关,与α、MA值呈正相关;Plt与K值呈负相关,与α、MA值呈正相关(P<0.01)。单因素分析:将得到8个输血前因素K、MA、PT、Fib、NEUT%、Hb、Alb、SCr做Logistic回归,建立输血预测模型,ROC曲线最佳输血阈值(患者输血预测值)为0.448,灵敏度92.9%,特异度88.9%,AUC0.969。结论综合上消化道出血患者TEG、凝血功能、血常规和生化等检测指标,建立Logistic回归模型对预测患者输血结局有明显的关联性,有较好的临床实用性。展开更多
Objective The research area is located in the north of the Xing＇an block and within the Ali River-Zhalantun granite belt in the eastern part of the Xingmeng orogenic belt. The geotectonic setting and geological evol...Objective The research area is located in the north of the Xing＇an block and within the Ali River-Zhalantun granite belt in the eastern part of the Xingmeng orogenic belt. The geotectonic setting and geological evolution history of this area are complex with strong magmatic activity and extremely developed granite rocks. Since predecessors have not obtained accurate dating result, there is much controversy over the formation of granitic rocks. Therefore, this work conducted zircon U-Ph dating on alkali-feldspar granite and granite porphyry in the northeast of Jiagedaqi to provide age constraint. The age data shows that the forming age is the Early Cretaceous and provides age basis for the tectonic evolution of the Da Hinggan Mountains.展开更多
The codon usage bias and the base compositions in the available 49 complete fish rhabdovirus genome sequences were investigated. The high correlation between GC12% and GC3% suggested that mutational pressure rather th...The codon usage bias and the base compositions in the available 49 complete fish rhabdovirus genome sequences were investigated. The high correlation between GC12% and GC3% suggested that mutational pressure rather than natural selection was the main factor determining the codon usage and base component in fish rhabdovirns, most of the spots lying below the expected curve （ ENC vs GC3% ） also suggested that codon usage bias in these 49 genomes was influenced by mutational pressure in codon usage pattern. Through PCA analysis based on RSCU, all ORFs in the 49 fish rhabdoviurs genomes were clustered into two parts, and this result showed that hosts played a role in codon usage pattern of fish rhabdovirns genomes. Further, comparison between SVCV, INHV, VHSV and their hosts suggested the effect of natural host on the codon usage pattern took part in the evolution process of fish rhabdovirns. This study represents the most comprehensive analysis to date for fish rhabdovirus eodon usage patterns.展开更多
Bi-activated photonic materials are promising for various applications in high-capacity telecommunication,tunable laser,and advanced bioimaging and sensing.Although various Bi-doped material candidates have been explo...Bi-activated photonic materials are promising for various applications in high-capacity telecommunication,tunable laser,and advanced bioimaging and sensing.Although various Bi-doped material candidates have been explored,manufacturing of Bi heavily doped fiber with excellent optical activity remains a long-standing challenge.Herein,a novel viscosity evolutional behavior mediated strategy for manufacturing of Bi-doped active fiber with high dopant solubility is proposed.The intrinsic relation among the evolution of Bi,reaction temperature and viscosity of the glass system is established.Importantly,the effective avenue to prevent the undesired deactivation of Bi during fiber drawing by tuning the temperature dependent viscosity evolution is built.By applying the strategy,for the first time we demonstrate the success in fabrication of heavily doped Bi active fiber.Furthermore,the principal fiber amplifier device is constructed and broadband optical signal amplification is realized.Our findings indicate the effectiveness of the proposed temperature dependent viscosity mediated strategy for developing novel photonic active fiber,and they also demonstrate the great potential for application in the next-generation high-capacity telecommunication system.展开更多
Marsdeniae tenacissimae extract(MTE), commonly known as Xiao-Ai-Ping in China, is a traditional Chinese herb medicine capable of inhibiting proliferation and metastasis and boosting apoptosis in various cancer cells. ...Marsdeniae tenacissimae extract(MTE), commonly known as Xiao-Ai-Ping in China, is a traditional Chinese herb medicine capable of inhibiting proliferation and metastasis and boosting apoptosis in various cancer cells. However, little is known about the contribution of MTE towards tumor angiogenesis and the underlying mechanism. The present study aimed to evaluate the effects of MTE on the proliferation and apoptosis of human umbilical vein endothelial cells(HUVECs) and the molecular ism. 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium, inner salt(MTS) and PI-stained flow cytometry assays revealed that MTE dose-dependently reduced the proliferation of HUVECs by arresting cell cycle at S phase(P < 0.05). Annexin V-FITC/PI-stained flow cytometry confirmed that MTE(160 μL·m L-1) enhanced the apoptosis of HUVECs significantly(P < 0.001). Real-time quantitative RT-PCR and Western blot analyses showed an increase in Bax expression and a sharply decline in Bcl-2 expression; caspase-3 was activated simultaneously in a dose-dependent manner(P < 0.05). Further study observed the dose-dependent down-regulation of vascular endothelial growth factor(VEGF) receptor-2(VEGFR-2), P2Y6 receptor(P2Y6R), and chemokine(C-C motif) ligand 2(CCL-2), along with the activation of PKC δ and up-regulation of p53 in a dose-dependent manner in MTE-treated selected cells(P < 0.05). Collectively, the results from the present study suggested that MTE suppressed the proliferation by attenuating CCL-2-mediated VEGF/VEGFR2 interactions and promoted the apoptosis through PKCδ-induced p53-dependent mitochondrial pathway in HUVECs, supporting that MTE may be developed as a potent anti-cancer medicine.展开更多
文摘目的建立应用血栓弹力图(TEG)及凝血指标综合评估上消化道出血患者凝血功能的输血结局预测模型。方法从输血(科)管理系统和医院信息系统(HIS)系统收集浙江省人民医院及其淳安分院消化内科2018年6月~2021年6月收治的101名符合临床诊断标准的上消化道出血患者,根据结局是否输血分为输血组(n=56)和未输血组(n=45),以及按照肝硬化与否分为肝硬化组(n=74)和非肝硬化组(n=27),同时收集40名非上消化道出血患者的阴性对照组。对比各组的TEG检测R、K、α、MA参数,凝血功能检测PT、INR、APTT、TT、Fib,血常规检测Hb、Plt、WBC、NEUT%,以及生化检测Alb、SCr、ALT、AST、GGT等指标(值);分析TEG指标与传统凝血功能指标的相关性;采用单因素和多因素分析,筛选输血相关因素建立预测模型。结果输血组与未输血组比较:TEG的K(min)为3.86±3.12 vs 2.50±1.47,α(°)为54.00±14.08 vs 61.05±10.88,MA(mm)为51.12±13.37 vs 58.26±11.08(P<0.01);凝血指标检测PT(s)为16.36±7.45 vs 13.44±1.50,Fib(g)为1.59±0.87 vs 2.35±1.09(P<0.01);血常规检测NEUT%为0.75±0.13 vs 0.66±0.15,Hb(g/L)为68.04±14.49 vs 100.73±22.92(P<0.01);生化检测Alb(g/L)为29.73±6.08 vs 33.73±7.19,SCr(nmol/L)为99.50±53.55 vs 76.25±19.28(P<0.01)。相关性分析:APTT与R、K值呈正相关,与α、MA值呈负相关;Fib与K值呈负相关,与α、MA值呈正相关;Plt与K值呈负相关,与α、MA值呈正相关(P<0.01)。单因素分析:将得到8个输血前因素K、MA、PT、Fib、NEUT%、Hb、Alb、SCr做Logistic回归,建立输血预测模型,ROC曲线最佳输血阈值(患者输血预测值)为0.448,灵敏度92.9%,特异度88.9%,AUC0.969。结论综合上消化道出血患者TEG、凝血功能、血常规和生化等检测指标,建立Logistic回归模型对预测患者输血结局有明显的关联性,有较好的临床实用性。
基金funded by the National Natural Science Foundation of China(grant No.1212011120671)
文摘Objective The research area is located in the north of the Xing＇an block and within the Ali River-Zhalantun granite belt in the eastern part of the Xingmeng orogenic belt. The geotectonic setting and geological evolution history of this area are complex with strong magmatic activity and extremely developed granite rocks. Since predecessors have not obtained accurate dating result, there is much controversy over the formation of granitic rocks. Therefore, this work conducted zircon U-Ph dating on alkali-feldspar granite and granite porphyry in the northeast of Jiagedaqi to provide age constraint. The age data shows that the forming age is the Early Cretaceous and provides age basis for the tectonic evolution of the Da Hinggan Mountains.
基金Supported by National Natural Science Foundation of China(No.31402347)Guangdong Special Marine Fishery Science and Technology Project(A201701C04)+3 种基金"Molecular epidemiological study of Cy HV-3 in Guangdong Province",2016 Guangdong Special Funds for Fish DiseasesGuangzhou Science and Technology Plan Projects(201707010216)Guangdong Science and Technology Plan Projects(2017A020224019)Guangdong Science and Technology Plan Projects(2016A020210029)
文摘The codon usage bias and the base compositions in the available 49 complete fish rhabdovirus genome sequences were investigated. The high correlation between GC12% and GC3% suggested that mutational pressure rather than natural selection was the main factor determining the codon usage and base component in fish rhabdovirns, most of the spots lying below the expected curve （ ENC vs GC3% ） also suggested that codon usage bias in these 49 genomes was influenced by mutational pressure in codon usage pattern. Through PCA analysis based on RSCU, all ORFs in the 49 fish rhabdoviurs genomes were clustered into two parts, and this result showed that hosts played a role in codon usage pattern of fish rhabdovirns genomes. Further, comparison between SVCV, INHV, VHSV and their hosts suggested the effect of natural host on the codon usage pattern took part in the evolution process of fish rhabdovirns. This study represents the most comprehensive analysis to date for fish rhabdovirus eodon usage patterns.
基金support from the National Key R&D Program of China(2020YFB1805901)the National Science Fund for Distinguished Young Scholars(62125502)+7 种基金the National Natural Science Foundation of China(51972113,52302002 and 62305115)the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01X137)Foshan Science and Technology Innovation Project(1920001000052)the Foundation of State Key Laboratory of Reactor System Design Technologythe Large Scientific Facility Open Subject of Songshan Lake,Dongguan,Guangdongthe Research Project supported by State Key Lab of Luminescent Materials and DevicesSouth China University of Technology(Skllmd2023-07)the Sponsored Research Project of Corning Incorporated。
文摘Bi-activated photonic materials are promising for various applications in high-capacity telecommunication,tunable laser,and advanced bioimaging and sensing.Although various Bi-doped material candidates have been explored,manufacturing of Bi heavily doped fiber with excellent optical activity remains a long-standing challenge.Herein,a novel viscosity evolutional behavior mediated strategy for manufacturing of Bi-doped active fiber with high dopant solubility is proposed.The intrinsic relation among the evolution of Bi,reaction temperature and viscosity of the glass system is established.Importantly,the effective avenue to prevent the undesired deactivation of Bi during fiber drawing by tuning the temperature dependent viscosity evolution is built.By applying the strategy,for the first time we demonstrate the success in fabrication of heavily doped Bi active fiber.Furthermore,the principal fiber amplifier device is constructed and broadband optical signal amplification is realized.Our findings indicate the effectiveness of the proposed temperature dependent viscosity mediated strategy for developing novel photonic active fiber,and they also demonstrate the great potential for application in the next-generation high-capacity telecommunication system.
基金supported by the National Natural Science Foundation of China(No.81501824)the Natural Science Foundation of Zhejiang Province(Nos.Y15H280010 and LY15C090004)+3 种基金the Analysis and Measurement Foundation of Zhejiang Province(No.2015C37001)the Key Project of Chinese medicine in Zhejiang Province(No.2015ZZ001)the Traditional Chinese Medicine Scientific Research and Outstanding Young Talent Foundation of Zhejiang Province(Nos.2014ZB007 and 2014ZQ005)the Medicine and Health Research Foundation of Zhejiang Province(Nos.2015ZDA002,2015KYA028,2014KYA233,2015KYB021,and 2017179748)
文摘Marsdeniae tenacissimae extract(MTE), commonly known as Xiao-Ai-Ping in China, is a traditional Chinese herb medicine capable of inhibiting proliferation and metastasis and boosting apoptosis in various cancer cells. However, little is known about the contribution of MTE towards tumor angiogenesis and the underlying mechanism. The present study aimed to evaluate the effects of MTE on the proliferation and apoptosis of human umbilical vein endothelial cells(HUVECs) and the molecular ism. 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium, inner salt(MTS) and PI-stained flow cytometry assays revealed that MTE dose-dependently reduced the proliferation of HUVECs by arresting cell cycle at S phase(P < 0.05). Annexin V-FITC/PI-stained flow cytometry confirmed that MTE(160 μL·m L-1) enhanced the apoptosis of HUVECs significantly(P < 0.001). Real-time quantitative RT-PCR and Western blot analyses showed an increase in Bax expression and a sharply decline in Bcl-2 expression; caspase-3 was activated simultaneously in a dose-dependent manner(P < 0.05). Further study observed the dose-dependent down-regulation of vascular endothelial growth factor(VEGF) receptor-2(VEGFR-2), P2Y6 receptor(P2Y6R), and chemokine(C-C motif) ligand 2(CCL-2), along with the activation of PKC δ and up-regulation of p53 in a dose-dependent manner in MTE-treated selected cells(P < 0.05). Collectively, the results from the present study suggested that MTE suppressed the proliferation by attenuating CCL-2-mediated VEGF/VEGFR2 interactions and promoted the apoptosis through PKCδ-induced p53-dependent mitochondrial pathway in HUVECs, supporting that MTE may be developed as a potent anti-cancer medicine.
