Fatty acid binding protein 5 is a novel therapeutic target for hepatocellular carcinoma

BACKGROUND Hepatocellular carcinoma(HCC)is an aggressive subtype of liver cancer and is one of the most common cancers with high mortality worldwide.Reprogrammed lipid metabolism plays crucial roles in HCC cancer cell survival,growth,and evolution.Emerging evidence suggests the importance of fatty acid binding proteins(FABPs)in contribution to cancer progression and metastasis;however,how these FABPs are dysregulated in cancer cells,especially in HCC,and the roles of FABPs in cancer progression have not been well defined.AIM To understand the genetic alterations and expression of FABPs and their associated cancer hallmarks and oncogenes in contributing to cancer malignancies.METHODS We used The Cancer Genome Atlas datasets of pan cancer and liver hepatocellular carcinoma(LIHC)as well as patient cohorts with other cancer types in this study.We investigated genetic alterations of FABPs in various cancer types.mRNA expression was used to determine if FABPs are abnormally expressed in tumor tissues compared to non-tumor controls and to investigate whether their expression correlates with patient clinical outcome,enriched cancer hallmarks and oncogenes previously reported for patients with HCC.We determined the protein levels of FABP5 and its correlated genes in two HCC cell lines and assessed the potential of FABP5 inhibition in treating HCC cells.RESULTS We discovered that a gene cluster including five FABP family members(FABP4,FABP5,FABP8,FABP9 and FABP12)is frequently co-amplified in cancer.Amplification,in fact,is the most common genetic alteration for FABPs,leading to overexpression of FABPs.FABP5 showed the greatest differential mRNA expression comparing tumor with non-tumor tissues.High FABP5 expression correlates well with worse patient outcomes(P<0.05).FABP5 expression highly correlates with enrichment of G2M checkpoint(r=0.33,P=1.1e-10),TP53 signaling pathway(r=0.22,P=1.7e-5)and many genes in the gene sets such as CDK1(r=0.56,P=0),CDK4(r=0.49,P=0),and TP53(r=0.22,P=1.6e-5).Furthermore,FABP5 also correlates well with two co-expressed oncogenes PLK1 and BIRC5 in pan cancer especially in LIHC patients(r=0.58,P=0;r=0.58,P=0;respectively).FABP5high Huh7 cells also expressed higher protein levels of p53,BIRC5,CDK1,CDK2,and CDK4 than FABP5low HepG2 cells.FABP5 inhibition more potently inhibited the tumor cell growth in Huh7 cells than in HepG2 cells.CONCLUSION We discovered that FABP5 gene is frequently amplified in cancer,especially in HCC,leading to its significant elevated expression in HCC.Its high expression correlates well with worse patient outcome,enriched cancer hallmarks and oncogenes in HCC.FABP5 inhibition impaired the cell viability of FABP5high Huh7 cells.All these support that FABP5 is a novel therapeutic target for treating FABP5high HCC.

Heteroatom-Induced Accelerated Kinetics on Nickel Selenide for Highly Efficient Hydrazine-Assisted Water Splitting and Zn-Hydrazine Battery

Hydrazine-assisted water electrolysis is a promising energy conversion technology for highly efficient hydrogen production.Rational design of bifunctional electrocatalysts,which can simultaneously accelerate hydrogen evolution reaction(HER)/hydrazine oxidation reaction(HzOR)kinetics,is the key step.Herein,we demonstrate the development of ultrathin P/Fe co-doped NiSe_(2) nanosheets supported on modified Ni foam(P/Fe-NiSe_(2)) synthesized through a facile electrodeposition process and subsequent heat treatment.Based on electrochemical measurements,characterizations,and density functional theory calculations,a favorable“2+2”reaction mechanism with a two-step HER process and a two-step HzOR step was fully proved and the specific effect of P doping on HzOR kinetics was investigated.P/Fe-NiSe_(2) thus yields an impressive electrocatalytic performance,delivering a high current density of 100 mA cm^(−2) with potentials of−168 and 200 mV for HER and HzOR,respectively.Additionally,P/Fe-NiSe_(2) can work efficiently for hydrazine-assisted water electrolysis and Zn-Hydrazine(Zn-Hz)battery,making it promising for practical application.

Numerical simulation of rock-breaking and influence laws of dynamic load parameters during axial-torsional coupled impact drilling with a single PDC cutter

Axial and torsional impact drilling technology is used to improve the drilling efficiency of hard rock formation in the deep underground.Still,the corresponding theory is not mature,and there are few correlative research reports on the rock-breaking mechanism of axial and torsional coupled impact drilling tools.Considering the influence of the impact hammer geometry and movement on the dynamic load parameters(i.e.,wavelength,amplitude,frequency,and phase difference),a numerical model that includes a hard formation and single polycrystalline diamond compact cutter was established.The Riedel-Hiermaier-Thoma model,which considers the dynamic damage and strength behavior of rocks,was adopted to analyze the rock damage under axial and torsional impact loads.The numerical simu-lation results were verified by the experimental results.It was found that compared with conventional drilling,the penetration depths of axial,torsional,and axial-torsional coupled impact drilling increased by 31.3%,5.6%,and 34.7%,respectively.Increasing the wavelength and amplitude of the axial impact stress wave improved the penetration depth.When the bit rotation speed remained unchanged,increasing the frequency in the axial and circumferential directions had little effect on the penetration depth.However,as the frequency increased,the cutting surface became increasingly smooth,which reduced the occurrence of bit vibration.When the phase difference between the axial and circumfer-ential stress waves was 25%,the penetration depth significantly increased.In addition,the bit vibration problem can be effectively reduced.Finally,the adjustment of engineering and tool structure parameters is proposed to optimize the efficiency of the axial-torsional coupled impact drilling tool.

急性脑卒中患者血浆Hcy与脑白质疏松、PSCAD的相关性分析

目的探讨急性脑卒中患者血浆同型半胱氨酸(Hcy)与脑白质疏松、卒中后焦虑抑郁共病(PSCAD)的关系。方法回顾性分析270例急性脑卒中患者临床资料,按照脑白质疏松(LA)严重程度分为轻度LA组和中重度LA组,按照是否存在PSCAD状态分为PSCAD组和非PSCAD组,所有患者均于入院后第2天抽取肘静脉血检测血浆Hcy水平,分析血浆Hcy水平与LA、PSCAD的关系。结果中重度LA组患者年龄、高血压、吸烟、中重度梗死及血浆Hcy水平高于轻度LA组(P<0.05),PSCAD组高血压、饮酒、基底节区和额叶区梗死、中重度梗死及血浆Hcy水平高于非PSCAD组(P<0.05);年龄、高血压及血浆Hcy水平是中重度LA的独立危险因素(P <0.05),饮酒、血浆Hcy水平是PSCAD的独立危险因素(P <0.05)。血浆Hcy水平与LA、PSCAD呈正相关(P<0.05)。结论通过检测血浆Hcy水平能够在一定程度上反应LA病情和焦虑抑郁程度,为临床治疗和预后监测提供依据。

Dual targeting of Polo-like kinase 1 and baculoviral inhibitor of apoptosis repeat-containing 5 in TP53-mutated hepatocellular carcinoma

BACKGROUND Hepatocellular carcinoma(HCC),often diagnosed at advanced stages without curative therapies,is the fifth most common malignant cancer and the second leading cause of cancer-related mortality.Polo-like kinase 1(PLK1)is activated in the late G2 phase of the cell cycle and is required for entry to mitosis.Interestingly,PLK1 is overexpressed in many HCC patients and is highly associated with poor clinical outcome.Baculoviral inhibitor of apoptosis repeatcontaining 5(BIRC5)is also highly overexpressed in HCC and plays key roles in this malignancy.AIM To determine the expression patterns of PLK1 and BIRC5,as well as their correlation with p53 mutation status and patient clinical outcome.METHODS The expression patterns of PLK1 and BIRC5,and their correlation with p53 mutation status or patient clinical outcome were analyzed using a TCGA HCC dataset.Cell viability,cell apoptosis,and cell cycle arrest assays were conducted to investigate the efficacy of the PLK1 inhibitors volasertib and GSK461364 and the BIRC5 inhibitor YM155,alone or in combination.The in vivo efficacy of volasertib and YM155,alone or in combination,was assessed in p53-mutated Huh7-derived xenograft models in immune-deficient NSIG mice.RESULTS Our bioinformatics analysis using a TCGA HCC dataset revealed that PLK1 and BIRC5 were overexpressed in the same patient subset and their expression was highly correlated.The overexpression of both PLK1 and BIRC5 was more frequently detected in HCC with p53 mutations.High PLK1 or BIRC5 expression significantly correlated with poor clinical outcome.PLK1 inhibitors(volasertib and GSK461364)or a BIRC5 inhibitor(YM155)selectively targeted Huh7 cells with mutated p53,but not HepG2 cells with wild-type p53.The combination treatment of volasertib and YM155 synergistically inhibited the viability of Huh7 cells via apoptotic pathway.The efficacy of volasertib and YM155,alone or in combination,was validated in vivo in a Huh7-derived xenograft model.CONCLUSION PLK1 and BIRC5 are highly co-expressed in p53-mutated HCC and inhibition of both PLK1 and BIRC5 synergistically compromises the viability of p53-mutated HCC cells in vitro and in vivo.

First-principles calculations of structural,elastic and electronic properties of(TaNb)0.67(HfZrTi)0.33 high-entropy alloy under high pressure

To clarify the effect of pressure on a(TaNb)0.67(HfZrTi)0.33 alloy composed of a solid solution with a single body-centered-cubic crystal structure,we used first-principles calculations to theoretically investigate the structural,elastic,and electronic properties of this alloy at different pressures.The results show that the calculated equilibrium lattice parameters are consistent with the experimental results,and that the normalized structural parameters of lattice constants and volume decrease whereas the total enthalpy differenceΔE and elastic constants increase with increasing pressure.The(TaNb)0.67(HfZrTi)0.33 alloy exhibits mechanical stability at high pressures lower than 400 GPa.At high pressure,the bulk modulus B shows larger values than the shear modulus G,and the alloy exhibits an obvious anisotropic feature at pressures ranging from 30 to 70 GPa.Our analysis of the electronic structures reveals that the atomic orbitals are occupied by the electrons change due to the compression of the crystal lattices under the effect of high pressure,which results in a decrease in the total density of states and a wider electron energy level.This factor is favorable for zero resistance.

Establishment of injury models in studies of biological effects induced by microwave radiation

Microwave radiation has been widely used in various fields,such as communication,industry,medical treatment,and military applications.Microwave radiation may cause injuries to both the structures and functions of various organs,such as the brain,heart,reproductive organs,and endocrine organs,which endanger human health.Therefore,it is both theoretically and clinically important to conduct studies on the biological effects induced by microwave radiation.The successful establishment of injury models is of great importance to the reliability and reproducibility of these studies.In this article,we review the microwave exposure conditions,subjects used to establish injury models,the methods used for the assessment of the injuries,and the indicators implemented to evaluate the success of injury model establishment in studies on biological effects induced by microwave radiation.

Insights into efficient transition metal-nitrogen/carbon oxygen reduction electrocatalysts

It is of vital importance to accelerate the sluggish oxygen reduction reaction(ORR)process at the cathode with earth-abundant metal-based catalysts for the commercialization of low-temperature polymer electrolyte membrane fuel cells.In consideration of high catalytic activity,long-term stability and low cost of potential ORR electrocatalysts,transition metal species have attracted much interest and transition metal-nitrogen-carbon(M-N/C,M=Fe,Co,Ni,Mn,etc.)catalysts have been widely considered as the most promising non-precious metal catalysts for ORR.Herein,the fundamental understanding of ORR catalytic mechanism and the identification of active centers are briefly introduced,and then different M-N/C catalysts classified by precursors with the strategies for design and optimization are highlighted.The challenges and possible opportunity for future development of high-performance ORR catalysts are finally proposed.

Synergistically enhanced activity and stability of bifunctional nickel phosphide/sulfide heterointerface electrodes for direct alkaline seawater electrolysis

Direct electrolysis of seawater to generate hydrogen is an attractive but challenging renewable energy storage technology.Reasonable design of seawater electrolysis catalysts should integrate high activity for hydrogen evolution reaction(HER)/oxygen evolution reaction(OER)and enhanced physical/electrochemical stability in seawater.Herein,we demonstrate the development of a Ni foam(NF)supported interfacial heterogeneous nickel phosphide/sulfide(Ni_(2)P/NiS_(2))microsphere electrocatalyst(NiPS/NF)through a facile electrodeposition and subsequent phosphorization/sulfuration process.After NiS_(2)modification,a charge redistribution on the heterointerface is demonstrated and a more advantageous covalent nature of the Ni-P bond is obtained for more easily adsorption of H*and H_(2)O.The NiPS/NF thus yields an impressive electrocatalytic performance in 1.0 M KOH,requiring small overpotentials of 169 and 320 mV for HER and OER to obtain a high current density of 100 m A cm^(-2),respectively.The NiPS/NF can also work efficiently in alkaline seawater with negligible activity degradation,requiring overpotentials of only 188 and 344 mV for a current density of 100 m A cm^(-2)for HER and OER,respectively.A synergistically enhanced physical/electrochemical long-term stability NiPS/NF in saline water is also demonstrated.

Correlation of pressure gradient in three hepatic veins with portal pressure gradient

BACKGROUND The liver is one of the most important organs in the human body,with functions such as detoxification,digestion,and blood coagulation.In terms of vascular anatomy,the liver is divided into the left and the right liver by the main portal vein,and there are three hepatic efferent veins(right,middle,and left)and two portal branches.Patients with impaired liver function have increased intrahepatic vascular resistance and splanchnic vasodilation,which may lead to an increase in the portal pressure gradient(PPG)and cause portal hypertension(PHT).In order to measure the increased pressure gradient of portal vein,the hepatic venous pressure gradient(HVPG)can be measured to reflect it in clinical practice.The accuracy of PPG measurements is directly related to patient prognosis.AIM To analyze the correlation between HVPG of three hepatic veins and PPG in patients with PHT.METHODS From January 2017 to December 2019,102 patients with PHT who met the inclusion criteria were evaluated during the transjugular intrahepatic portosystemic shunt procedure and analyzed.RESULTS The mean HVPG of the middle hepatic vein was 17.47±10.25 mmHg,and the mean HVPG of the right and left hepatic veins was 16.34±7.60 and 16.52±8.15 mmHg,respectively.The average PPG was 26.03±9.24 mmHg.The correlation coefficient and coefficient of determination of the right hepatic vein,middle hepatic vein,and left hepatic vein were 0.15 and 0.02(P=0.164);0.25 and 0.05(P=0.013);and 0.14 and 0.02(P=0.013),respectively.The mean wedged hepatic vein/venous pressure(WHVP)of the middle and left hepatic veins was similar at 29.71±12.48 and 29.1±10.91 mmHg,respectively,and the mean WHVP of the right hepatic vein was slightly lower at 28.01±8.95 mmHg.The mean portal vein pressure was 34.11±8.56 mmHg.The correlation coefficient and coefficient of determination of the right hepatic vein,middle hepatic vein,and left hepatic vein were 0.26 and 0.07(P=0.009);0.38 and 0.15(P<0.001);and 0.26 and 0.07(P=0.008),respectively.The average free hepatic venous pressure(FHVP)of the right hepatic vein was lowest at 11.67±5.34 mmHg,and the average FHVP of the middle and left hepatic veins was slightly higher at 12.19±4.88 and 11.67±5.34 mmHg,respectively.The average inferior vena cava pressure was 8.27±4.04 mmHg.The correlation coefficient and coefficient of determination of the right hepatic vein,middle hepatic vein,and left hepatic vein were 0.30 and 0.09(P=0.002);0.18 and 0.03(P=0.078);and 0.16 and 0.03(P=0.111),respectively.CONCLUSION Measurement of the middle hepatic vein HVPG could better represent PPG.Considering the high success rate of clinical measurement of the right hepatic vein,it can be the second choice.

Scanning photoacoustic imaging of submucosal gastric tumor based on a long focused transducer in phantom and in vitro experiments

Photoacoustic imaging,which can provide the maximum intensity contrast in tissue depth imaging without ionizing radiation,will be a promising imaging trend for tumor detection.In this paper,a column diffusionfiber was employed to carry a pulsed laser for irradiating stomach directly through esophagus based on the characteristics of gastric tissue structure.A long focused ultrasonic transducer was placed outside the body to detect photoacoustic signals of gastric tissue.Phantom and in vitro experiments of submucosal gastric tumors were carried out to check the sensitivity of scanning photoacoustic tomography system,including the lateral and longitudinal resolution of the system,sensitivity of different absorption coefficient in imaging,capability of transversal detection,and probability of longitudinal detection.The results demonstrate that our innovative technique can improve the parameters of imaging.The lateral resolution reaches 2.09 mm.Then a depth of 5.5mm with a longitudinal accuracy of 0.36mm below gastric mucosa of early gastric cancer(EGC)has been achieved.In addition,the optimal absorption coefficient differences among absorbers of system are 3.3-3.9 times.Results indicate that our photoacoustic imaging(PAI)system,is based on a long focusing transducer,can provide a potential application for detecting submucosal EGC without obvious symptoms.

Application of machine learning algorithm for predicting gestational diabetes mellitus in early pregnancy

Objective:To study the application of a machine learning algorithm for predicting gestational diabetes mellitus(GDM)in early pregnancy.Methods:This study identified indicators related to GDM through a literature review and expert discussion.Pregnant women who had attended medical institutions for an antenatal examination from November 2017 to August 2018 were selected for analysis,and the collected indicators were retrospectively analyzed.Based on Python,the indicators were classified and modeled using a random forest regression algorithm,and the performance of the prediction model was analyzed.Results:We obtained 4806 analyzable data from 1625 pregnant women.Among these,3265 samples with all 67 indicators were used to establish data set F1;4806 samples with 38 identical indicators were used to establish data set F2.Each of F1 and F2 was used for training the random forest algorithm.The overall predictive accuracy of the F1 model was 93.10%,area under the receiver operating characteristic curve(AUC)was 0.66,and the predictive accuracy of GDM-positive cases was 37.10%.The corresponding values for the F2 model were 88.70%,0.87,and 79.44%.The results thus showed that the F2 prediction model performed better than the F1 model.To explore the impact of sacrificial indicators on GDM prediction,the F3 data set was established using 3265 samples(F1)with 38 indicators(F2).After training,the overall predictive accuracy of the F3 model was 91.60%,AUC was 0.58,and the predictive accuracy of positive cases was 15.85%.Conclusions:In this study,a model for predicting GDM with several input variables(e.g.,physical examination,past history,personal history,family history,and laboratory indicators)was established using a random forest regression algorithm.The trained prediction model exhibited a good performance and is valuable as a reference for predicting GDM in women at an early stage of pregnancy.In addition,there are cer tain requirements for the propor tions of negative and positive cases in sample data sets when the random forest algorithm is applied to the early prediction of GDM.

Hoffa's fracture in a five-year-old child diagnosed and treated with the assistance of arthroscopy:A case report

BACKGROUND Hoffa’s fracture is a coronal-oriented fracture of the femoral condyle.It is rarely observed in pediatric patients that isolated coronal fracture of the medial femoral condyle accompanies an intact lateral femoral condyle.Only a few cases involving Hoffa’s fracture of the medial femoral condyle have been reported in patients with undeveloped skeletons.Such a fracture cannot be observed by routine imaging examinations,thus resulting in possible misdiagnosis and further treatment challenges.CASE SUMMARY A 5-year-old boy with Hoffa’s fracture of the medial femoral condyle suffered from right knee pain and severe swelling after being hit by a heavy object.The patient was misdiagnosed and initially treated in a local primary healthcare center.No improvement in his right knee’s extension was observed following conservative treatment for 2 wk.The patient was transferred to our hospital,rediagnosed using arthroscopy,and underwent open reduction and internal fixation.The therapeutic outcome was satisfactory with the screws removed 7 mo after fixation.At the final follow-up of 40 mo,the range of motion in the knee had recovered.There was no varus-valgus instability.CONCLUSION Hoffa’s fracture is rarely seen in children aged 5 years,let alone in the medial condyle,and can easily be misdiagnosed due to limited physical and imaging examinations.Suspected Hoffa’s fracture in preschool children should be confirmed based on arthroscopic findings.Open reduction and internal fixation should be performed to protect the articular surface and prevent long-term complications.

Myeloid sarcoma with ulnar nerve entrapment:A case report

BACKGROUND Myeloid sarcoma(MS) is relatively rare,occurring mainly in the skin and lymph nodes,and MS invasion of the ulnar nerve is particularly unusual.The main aim of this article is to present a case of MS invading the brachial plexus,causing ulnar nerve entrapment syndrome,and to further clinical understanding of the possibility of MS invasion of peripheral nerves.CASE SUMMARY We present the case of a 46-year-old man with a 13-year history of well-treated acute nonlymphocytic leukaemia who was admitted to the hospital after presenting with numbness and pain in his left little finger.The initial diagnosis was considered a simple case of nerve entrapment disease,with magnetic resonance imaging showing slightly abnormal left brachial plexus nerve alignment with local thickening,entrapment,and high signal on compression lipid images.Due to the severity of the ulnar nerve compression,we surgically investigated and cleared the entrapment and nerve tissue hyperplasia;however,subsequent pathological biopsy results revealed evidence of MS.The patient had significant relief from his neurological symptoms,with no postoperative complications,and was referred to the haemato-oncology department for further consultation about the primary disease.This is the first report of safe treatment of ulnar nerve entrapment from MS.It is intended to inform hand surgeons that nerve entrapment may be associated with extramedullary MS,as a rare presenting feature of the disease.CONCLUSION MS invasion of the brachial plexus and surrounding tissues of the upper arm,resulting in ulnar nerve entrapment and degeneration with significant neurological pain and numbness in the little finger,is uncommon.Surgical treatment significantly relieved the patient’s nerve entrapment symptoms and prevented further neurological impairment.This case is reported to highlight the rare presenting features of MS.

A novel analysis of Scheimpflug total corneal refractive power following corneal cross-linking in mild to moderate keratoconus

AIM:To detect an earlier improvement in mild to moderate keratoconus following corneal cross-linking(CXL)with total corneal refractive power(TCRP)using ray tracing method.METHODS:A total of 40 eyes of 30 consecutive patients who underwent CXL for progressive keratoconus were retrospectively enrolled.The following keratometric parameters provided by Pentacam HR,including maximum keratometry(Kmax),steepest keratometry(Ksteep),3 mm zonal TCRP centered over corneal apex(TCRPapex,zone 3 mm),zonal mean keratometry and TCRP centered over corneal cone(Kmcone,zone and TCRPcone,zone 1,2,3 mm)were evaluated preoperatively and 1,3,6,and 12 mo postoperatively.Groups 1 and 2 were defined based on Kmax at postoperative 1 mo as improved(the initial improvement group)or worsen(the initial deterioration group)compared to the preoperative level.RESULTS:In the overall group,only keratometric parameters based on ray tracing method displayed significant improvement early at 3 mo postoperatively,in which TCRPcone,zone 1 mm and 2 mm exhibited the largest flattening(0.57 D and 0.53 D,respectively).In Group 1,only Kmax,Kmcone,zone 2 mm and TCRPcone,zone 2 mm showed significant improvement initially at 1 mo postoperatively,in which Kmax exhibited the largest improvement(1.05 D),followed by TCRPcone,zone 2 mm(0.82 D).In Group 2,only keratometric parameters based on ray tracing method and Kmcone,zone 3 mm showed slight but not significant improvement early at 3 mo,in which TCRPcone,zone 3 mm displayed the most improvement(0.19 D),followed by TCRPcone,zone 2 mm(0.15 D).CONCLUSION:The findings indicate that a 2 mm zonal TCRP centered over Kmax could earlier detect keratometric improvement by CXL compared to other commonly used parameters in mild to moderate keratoconic eyes.

Giant schwannoma of thoracic vertebra: A case report

BACKGROUND It is relatively rare for schwannomas to invade bone,but it is very rare for a large mass to form concurrently in the paravertebral region.Surgical resection is the only effective treatment.Because of the extensive tumor involvement and the many important surrounding structures,the tumor needs to be fully exposed.Most of the tumors are completely removed by posterior combined open-heart surgery to relieve spinal cord compression,restore the stability of the spine and maximize the recovery of nerve and spinal cord function.The main objective of this article is to present a schwannoma that had invaded the T5 and T6 vertebral bodies and formed a large paravertebral mass with simultaneous invasion of the spinal canal and compression of the spinal cord.CASE SUMMARY A 40-year-old female suffered from intermittent chest and back pain for 8 years.Computed tomography and magnetic resonance imaging scans showed a paravertebral tumor of approximately 86 mm×109 mm×116 mm,where the adjacent T5 and T6 vertebral bodies were invaded by the tumor,the right intervertebral foramen was enlarged,and the tumor had invaded the spinal canal to compress the thoracic medulla.The preoperative puncture biopsy diagnosed a benign schwannoma.Complete resection of the tumor was achieved by a two-step operation.In the first step,the thoracic surgeon adopted a lateral approach to separate the thoracic tumor from the lung.In the second step,a spine surgeon performed a posterior midline approach to dissect the tumor from the vertebral junction through removal of the tumor from the posterior side and further resection of the entire T5 and T6 vertebral bodies.The large bone defect was reconstructed with titanium mesh,and the posterior root arch was nail-fixed.Due to the large amount of intraoperative bleeding,we performed tumor angioembolization before surgery to reduce and avoid large intraoperative bleeding.The postoperative diagnosis of benign schwannoma was confirmed by histochemical examination.There was no sign of tumor recurrence or spinal instability during the 2-year follow-up.CONCLUSION Giant schwannoma is uncommon.In this case,a complete surgical resection of a giant thoracic nerve sheath tumor that invaded part of the vertebral body and compressed the spinal cord was safe and effective.

经双腔引流管大容量灌洗并持续负压引流用于感染性胰腺坏死的疗效分析

背景感染性胰腺坏死临床多采用手术治疗.然而,术后残余组织的引流仍存在引流不充分,引流管易堵塞等问题.本研究拟采取胰周双腔引流管大容量灌洗并持续负压引流的方法,并与同期采用普通引流的患者相比较.目的探讨胰周双腔引流管大容量灌洗并持续负压引流用于感染性胰腺坏死(infected pancreatic necrosis,IPN)的临床疗效.方法回顾性分析铜川市人民医院肝胆胰脾外科2015-06/2019-06收治的57例行手术治疗的感染性胰腺坏死患者的临床资料.按照术后冲洗引流方式不同,分为双腔引流管组和普通引流管组.双腔引流管组患者术中放置胰周双腔引流管并在术后行大容量灌洗并持续负压引流;普通引流管组患者术中放置小网膜囊引流管并术后行常规冲洗和引流.观察并比较两组患者治疗效果及引流情况.结果两组在人口统计学数据、胰腺炎的病因和疾病严重程度、手术时间、术中失血量、重症护理时间方面无明显差异.两组术后持续器官衰竭逆转数(84.21%vs 56.25%,P=0.13),严重并发症发生率(16.67%vs 33.33%,P=0.14)无明显差异;总体死亡率为12.28%.尽管无显著的统计学差异(P=0.17),但普通引流管组患者死亡率更高.双腔引流管组APACHEⅡ评分减少幅度更大(8.03±2.13 vs 3.85±1.29,P<0.01),无再手术病例(0 vs 22.22%,P=0.008),住院时间更短,引流管更通畅,能够更早的拔除引流管(P<0.01).结论经双腔引流管大容量灌洗并持持续负压引流是IPN的有效引流方法,可有效治疗IPN.

Application of steam injection in iron ore sintering:fuel combustion efficiency and CO emissions

Improving the combustion efficiency of fuels is essential to reducing pollutant emissions in the iron ore sintering process.The sintering bed surface steam-injection technology has attracted significant research interest for its potential advantages in low-energy consumption and low emission.The effect of steam injection on fuel combustion efficiency and CO emission was studied by comparing the thermodynamic response from the sintering process before and after steam injection.The mechanism of improving combustion efficiency was also revealed.The results indicated that the sintering gas medium of H_(2)O-H_(2)-N_(2)-O_(2) with the blown steam improved the heat transfer conditions of fuel combustion and promoted the water gas reaction.The optimum state of steam injection was achieved at 15 min after ignition with 0.02 m^(3) min^(-1).The CO emission reduction is 10.91% compared with the base case.The combustion efficiency was 88.83%,6.15% higher than conventional sintering,and the solid fuel consumption was reduced by 1.15 kg t^(-1).It was indicated that steam injection would improve combustion efficiency and reduce solid fuel consumption.Meanwhile,the steam injection could improve the combustion kinetic conditions in the zone of unburned fuel and low oxygen partial pressure.It was conducive to the reaction of H_(2)O with C and CO to convert the CO of reducing atmosphere to CO_(2),which in turn realized the complete combustion of fuel and CO and improved the efficiency of fuel combustion.

“Charging”the cigarette butt:heteroatomic porous carbon nanosheets with edge-induced topological defects for enhanced oxygen evolution performance

Owing to the complexity of electron transfer pathways,the sluggish oxygen evolution reaction process is defined as the bottleneck for the practical application of Zn-air batteries.In this effort,metal nanoparticles(Co,Ni,Fe,etc.)encapsulated within nitrogen-doped carbon materials with abundant edge sites were synthesized by one-step pyrolysis treatment using cigarette butts as raw materials,which can drastically accelerate the overall rate of oxygen evolution reaction by facilitating the adsorption of oxygenated intermediates by the edge-induced topological defects.The prepared catalyst of nitrogen-doped carbon porous nanosheets loaded with Co nanoparticles(Co@NC-500)exhibits enhanced catalytic activity toward oxygen evolution reaction,with a low overpotential of 350 mV at the current density of 10 mA·cm^(-2).Furthermore,the Zn-air battery assembled with Co@NC-500 catalyst demonstrates a desirable performance affording an open-circuit potential of 1.336 V and power density of 33.6 mW·cm^(-2),indicating considerable practical application potential.

Uncovering the prominent role of satellite cells in paravertebral muscle development and aging by single-nucleus RNA sequencing

To uncover the role of satellite cells(SCs)in paravertebral muscle development and aging,we constructed a single-nucleus transcriptomic atlas of mouse paravertebral muscle across seven timepoints spanning the embryo(day 16.5)to old(month 24)stages.Eight cell types,including SCs,fast muscle cells,and slow muscle cells,were identified.An energy metabolism-related gene set,TCA CYCLE IN SENESCENCE,was enriched in SCs.Forty-two skeletal muscle disease-related genes were highly expressed in SCs and exhibited similar expression patterns.Among them,Pdha1 was the core gene in the TCA CYCLE IN SENESCENCE;Pgam2,Sod1,and Suclg1 are transcription factors closely associated with skeletal muscle energy metabolism.Transcription factor enrichment analysis of the 42 genes revealed that Myod1 and Mef2a were also highly expressed in SCs,which regulated Pdha1 expression and were associated with skeletal muscle development.These findings hint that energy metabolism may be pivotal in SCs development and aging.Three ligand-receptor pairs of extracellular matrix(ECM)-receptor interactions,Lamc1-Dag1,Lama2-Dag1,and Hspg2-Dag1,may play a vital role in SCs interactions with slow/fast muscle cells and SCs self-renewal.Finally,we built the first database of a skeletal muscle single-cell transcriptome,the Musculoskeletal Cell Atlas(http://www.mskca.tech),which lists 630,040 skeletal muscle cells and provides interactive visualization,a useful resource for revealing skeletal muscle cellular heterogeneity during development and aging.Our study could provide new targets and ideas for developing drugs to inhibit skeletal muscle aging and treat skeletal muscle diseases.