Stem cell therapies have made great progress in the treatment of diabetic wounds during recent decades,while their short in vivo residence,alloimmune reactions,undesired behaviors,and dramatic losses of cell functions...Stem cell therapies have made great progress in the treatment of diabetic wounds during recent decades,while their short in vivo residence,alloimmune reactions,undesired behaviors,and dramatic losses of cell functions still hinder the translation of them into clinic.Here,inspired by the natural components of stem cell niches,we presented novel microfluidic hydrogel microcarriers with extracellular matrix(ECM)-like composition and adipose-derived stem cells(ADSCs)encapsulation for diabetic wound healing.As the hydrogel was synthesized by conjugating hyaluronic acid methacryloyl(HAMA)onto the Fibronectin(FN)molecule chain(FN-HAMA),the laden ADSCs in the microcarriers showed improved bioactivities and pro-regenerative capabilities.Based on these features,we have demonstrated that these ADSCs microcarriers exhibited significant promotion of neovascularization,follicular rejuvenation,and collagen deposition in a mouse diabetic wound model.These results indicated that the stem cell niche-inspired FN-HAMA microcarriers with ADSCs encapsulation have great clinical potential for diabetic wound treatment.展开更多
Poly-L-lysine(PLL)is a water-soluble biopolymer consist of repeating unit of L-lysine.It displays intrinsic non-antigenicity,antibacterial property,biocompatibility,and biodegradability,and facile synthesis procedure,...Poly-L-lysine(PLL)is a water-soluble biopolymer consist of repeating unit of L-lysine.It displays intrinsic non-antigenicity,antibacterial property,biocompatibility,and biodegradability,and facile synthesis procedure,there-fore has attracted particular attention in various biomedical and pharmaceutical applications.Following a brief introduction to PLL synthesis,this review focuses on the significant advances of PLL and PLL-derived complexes utilized in cargo delivery,tissue adhesives,and antibacterial materials.Meanwhile,this review summarized the modification and optimization of PLL complexes for further clinical translational studies.The final section dis-cusses the challenges and opportunities of PLL-based complexes in the biomedical areas.展开更多
Chronic wounds are characterized by prolonged healing processes and poor prognoses,which have substantially impacted human health and daily life.Traditional treatment strategies have various limitations and drawbacks....Chronic wounds are characterized by prolonged healing processes and poor prognoses,which have substantially impacted human health and daily life.Traditional treatment strategies have various limitations and drawbacks.Therefore,fully effective therapeutic approaches remain urgently needed.Stem cell(SC)-based therapies have drawn significant attention for their abilities of immunomodulation and pro-regeneration.It has been demon-strated that stem cells(SCs)can improve angiogenesis,collagen deposition,and hair rejuvenation,thus facilitating wound healing.In addition,attempts were performed to facilitate the cell survival,function,retention,and en-graftment of the delivered SCs.In this review,we first introduce the pathological process involved in chronic wound healing.Following that,the mechanism of SCs in promoting chronic wound repair is discussed in detail.Then,we highlight recent SC-based therapies for chronic wound repair developments.Finally,we present our views on the remaining challenges and future trends of SC-based therapies for chronic wound treatment.展开更多
基金supported by the National Key Research and Development Program of China(2020YFA0908200)the National Natural Science Foundation of China(81974288,T2225003 and 52073060)+2 种基金the Strategic Priority Research Program of the Chinese Academy of Science(XDA16021103)the Guangdong Basic and Applied Basic Research Foundation(2021B1515120054)the Shenzhen Fundamental Research Program(JCYJ20190813152616459 and JCYJ20210324133214038).
文摘Stem cell therapies have made great progress in the treatment of diabetic wounds during recent decades,while their short in vivo residence,alloimmune reactions,undesired behaviors,and dramatic losses of cell functions still hinder the translation of them into clinic.Here,inspired by the natural components of stem cell niches,we presented novel microfluidic hydrogel microcarriers with extracellular matrix(ECM)-like composition and adipose-derived stem cells(ADSCs)encapsulation for diabetic wound healing.As the hydrogel was synthesized by conjugating hyaluronic acid methacryloyl(HAMA)onto the Fibronectin(FN)molecule chain(FN-HAMA),the laden ADSCs in the microcarriers showed improved bioactivities and pro-regenerative capabilities.Based on these features,we have demonstrated that these ADSCs microcarriers exhibited significant promotion of neovascularization,follicular rejuvenation,and collagen deposition in a mouse diabetic wound model.These results indicated that the stem cell niche-inspired FN-HAMA microcarriers with ADSCs encapsulation have great clinical potential for diabetic wound treatment.
基金supported by the Natural Science Foundation of Jiangsu Province (BK20210016)the National Key R&D Program of China (2020YFA0710800)+2 种基金the Key Program of National Natural Science Foundation of China (grant no.81930043)the Major International (Regional)Joint Research Project of China (grant no.81720108020)the Jiangsu Provincial Key Research and Development Program (BE2020621).
文摘Poly-L-lysine(PLL)is a water-soluble biopolymer consist of repeating unit of L-lysine.It displays intrinsic non-antigenicity,antibacterial property,biocompatibility,and biodegradability,and facile synthesis procedure,there-fore has attracted particular attention in various biomedical and pharmaceutical applications.Following a brief introduction to PLL synthesis,this review focuses on the significant advances of PLL and PLL-derived complexes utilized in cargo delivery,tissue adhesives,and antibacterial materials.Meanwhile,this review summarized the modification and optimization of PLL complexes for further clinical translational studies.The final section dis-cusses the challenges and opportunities of PLL-based complexes in the biomedical areas.
基金supported by grants from the National Natural Science Foundation of China (No.81974288)the National Natural Science Foundation of China (No.82101184)+1 种基金Shenzhen Fundamental Research Program (No.JCYJ20210324102809024)Shenzhen PhD Start-up Program (No.RCBS20210609103713045).
文摘Chronic wounds are characterized by prolonged healing processes and poor prognoses,which have substantially impacted human health and daily life.Traditional treatment strategies have various limitations and drawbacks.Therefore,fully effective therapeutic approaches remain urgently needed.Stem cell(SC)-based therapies have drawn significant attention for their abilities of immunomodulation and pro-regeneration.It has been demon-strated that stem cells(SCs)can improve angiogenesis,collagen deposition,and hair rejuvenation,thus facilitating wound healing.In addition,attempts were performed to facilitate the cell survival,function,retention,and en-graftment of the delivered SCs.In this review,we first introduce the pathological process involved in chronic wound healing.Following that,the mechanism of SCs in promoting chronic wound repair is discussed in detail.Then,we highlight recent SC-based therapies for chronic wound repair developments.Finally,we present our views on the remaining challenges and future trends of SC-based therapies for chronic wound treatment.