Stem cell-derived spinal cord organoids(SCOs)have revolutionised the study of spinal cord development and disease mechanisms,offering a three-dimensional model that recapitulates the complexity of native tissue.This r...Stem cell-derived spinal cord organoids(SCOs)have revolutionised the study of spinal cord development and disease mechanisms,offering a three-dimensional model that recapitulates the complexity of native tissue.This review synthesises recent advancements in SCO technology,highlighting their role in modelling spinal cord morphogenesis and their application in neurodegenerative disease research.We discuss the methodological breakthroughs in inducing regional specification and cellular diversity within SCOs,which have enhanced their predictive ability for drug screening and their relevance in mimicking pathological conditions such as neurodegenerative diseases and neuromuscular disorders.Despite these strides,challenges in achieving vascularisation and mature neuronal integration persist.The future of SCOs lies in addressing these limitations,potentially leading to transformative impactions in regenerative medicine and therapeutic development.展开更多
基金financially supported by the National Science Fund for Distinguished Young Scholars(No.82225027)the National Key Research and Development Program(No.2021YFA1101301)+1 种基金the National Natural Science Foundation of China(Nos.82271419,82202702,82202351,82001308,and 82271418)Shanghai Rising-Star Program(No.22QA1408200).
文摘Stem cell-derived spinal cord organoids(SCOs)have revolutionised the study of spinal cord development and disease mechanisms,offering a three-dimensional model that recapitulates the complexity of native tissue.This review synthesises recent advancements in SCO technology,highlighting their role in modelling spinal cord morphogenesis and their application in neurodegenerative disease research.We discuss the methodological breakthroughs in inducing regional specification and cellular diversity within SCOs,which have enhanced their predictive ability for drug screening and their relevance in mimicking pathological conditions such as neurodegenerative diseases and neuromuscular disorders.Despite these strides,challenges in achieving vascularisation and mature neuronal integration persist.The future of SCOs lies in addressing these limitations,potentially leading to transformative impactions in regenerative medicine and therapeutic development.
