Casi2b/C2ci is a newly identified class 2 CRISPR endonuclease that was recently engineered for targeted genome editing in mammals and rice.To explore the potential applications of the CRISPR-Casi2b system in the dicot...Casi2b/C2ci is a newly identified class 2 CRISPR endonuclease that was recently engineered for targeted genome editing in mammals and rice.To explore the potential applications of the CRISPR-Casi2b system in the dicot Arabidopsis thaliana,we selected BvCasi2b and BhCasi2b v4 for analysis.We successfully used both endonucleases to induce mutations,perform multiplex genome editing,and create large deletions at multiple loci.No significant mutations were detected at potential off-target sites.Analysis of the insertion/deletion frequencies and patterns of mutants generated via targeted gene mutagenesis highlighted the potential utility of CRISPRCasi2b systems for genome editing in Arabidopsis.展开更多
基金This work was supported by the National Natural Science Foundation of China(31570190,31800224)the Science Technology and Innovation Committee of Shenzhen Municipality(JCYJ20170303154319837,JCYJ20170412155447658)+1 种基金the Natural Science Foundation of Hebei Province(12017208087)the Science and Technology Research Program for Colleges and Universities in Hebei Province(QN2018149).
文摘Casi2b/C2ci is a newly identified class 2 CRISPR endonuclease that was recently engineered for targeted genome editing in mammals and rice.To explore the potential applications of the CRISPR-Casi2b system in the dicot Arabidopsis thaliana,we selected BvCasi2b and BhCasi2b v4 for analysis.We successfully used both endonucleases to induce mutations,perform multiplex genome editing,and create large deletions at multiple loci.No significant mutations were detected at potential off-target sites.Analysis of the insertion/deletion frequencies and patterns of mutants generated via targeted gene mutagenesis highlighted the potential utility of CRISPRCasi2b systems for genome editing in Arabidopsis.
