Overexpression of heat shock protein 27(HSP27)in gastric cancer is correlated with poor clinical prognosis.Melatonin,an endogenous hormone,shows promise in gastric cancer therapy.However,there is limited study on the ...Overexpression of heat shock protein 27(HSP27)in gastric cancer is correlated with poor clinical prognosis.Melatonin,an endogenous hormone,shows promise in gastric cancer therapy.However,there is limited study on the biological activity of HSP27 in response to melatonin treatment.In this study,we show an anti-proliferative action of melatonin on human gastric cancer cell lines BGC-823 and MGC-803.Biochemically,the inhibitory effect of melatonin is accompanied by the upregulation of HSP27 phosphorylation level.Transfection of gastric cancer cells with HSP27-specific siRNA effectively reduces HSP27 phosphorylation and potentiated melatonininduced inhibitory effect on cell proliferation.The reduction of cyclin D1 in melatonin-treated cells is also aggravated by HSP27 depletion.Moreover,melatonin stimulation increases p38 phosphorylation.Pretreatment with p38 inhibitor SB203580 not only remarkably suppresses melatonin-induced HSP27 phosphorylation,but also augment the inhibitory effect of melatonin on cyclin D1 expression as well as cell proliferation.Taken together,our study indicates the protective pathway of p38/HSP27 against melatonin-induced inhibitory effect on gastric cancer cell proliferation,suggesting that combined with p38/HSP27 pathway inhibitor,the therapeutic efficacy of melatonin on gastric cancer may be improved.展开更多
Both the modeling and the load regulation capacity prediction of a supercritical power plant are investigated in this paper. Firstly, an indirect identification method based on subspace identification method is propos...Both the modeling and the load regulation capacity prediction of a supercritical power plant are investigated in this paper. Firstly, an indirect identification method based on subspace identification method is proposed. The obtained identification model is verified by the actual operation data and the dynamic characteristics of the system are well reproduced. Secondly, the model is used to predict the load regulation capacity of thermal power unit. The power, main steam pressure, main steam temperature and other parameters are simulated respectively when the unit load is going up and down. Under the actual constraints, the load regulation capacity of thermal power unit can be predicted quickly.展开更多
基金supported by the Training Programs of Innovation and Entrepreneurship for Undergraduates by Jiangsu Province(201810312018Z)to Jun Du,Chenchen Zhu,Kaiquan Lithe Training Programs of Innovation and Entrepreneurship for Undergraduates by Nanjing Medical University to Jun Du,Yuting Wang
文摘Overexpression of heat shock protein 27(HSP27)in gastric cancer is correlated with poor clinical prognosis.Melatonin,an endogenous hormone,shows promise in gastric cancer therapy.However,there is limited study on the biological activity of HSP27 in response to melatonin treatment.In this study,we show an anti-proliferative action of melatonin on human gastric cancer cell lines BGC-823 and MGC-803.Biochemically,the inhibitory effect of melatonin is accompanied by the upregulation of HSP27 phosphorylation level.Transfection of gastric cancer cells with HSP27-specific siRNA effectively reduces HSP27 phosphorylation and potentiated melatonininduced inhibitory effect on cell proliferation.The reduction of cyclin D1 in melatonin-treated cells is also aggravated by HSP27 depletion.Moreover,melatonin stimulation increases p38 phosphorylation.Pretreatment with p38 inhibitor SB203580 not only remarkably suppresses melatonin-induced HSP27 phosphorylation,but also augment the inhibitory effect of melatonin on cyclin D1 expression as well as cell proliferation.Taken together,our study indicates the protective pathway of p38/HSP27 against melatonin-induced inhibitory effect on gastric cancer cell proliferation,suggesting that combined with p38/HSP27 pathway inhibitor,the therapeutic efficacy of melatonin on gastric cancer may be improved.
文摘Both the modeling and the load regulation capacity prediction of a supercritical power plant are investigated in this paper. Firstly, an indirect identification method based on subspace identification method is proposed. The obtained identification model is verified by the actual operation data and the dynamic characteristics of the system are well reproduced. Secondly, the model is used to predict the load regulation capacity of thermal power unit. The power, main steam pressure, main steam temperature and other parameters are simulated respectively when the unit load is going up and down. Under the actual constraints, the load regulation capacity of thermal power unit can be predicted quickly.
