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EAEC噬菌体PNJ1809-11和PNJ1809-13作为环境消毒剂的杀菌效果评估 被引量:5
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作者 钱新杰 李一昊 +8 位作者 曾颃 殷晗杰 王瑜欣 黄豪圣 巩倩雯 李德志 薛峰 汤芳 戴建君 《微生物学报》 CAS CSCD 北大核心 2021年第7期2018-2030,共13页
【目的】分析2株肠聚集性大肠杆菌(EAEC)CVCC232噬菌体PNJ1809-11、PNJ1809-13的生物学特性,并对其作为环境消毒剂的杀菌效果进行评估。【方法】透射电镜下观察PNJ1809-11、PNJ1809-13的形态;通过宿主谱、最佳感染复数(MOI)、一步生长... 【目的】分析2株肠聚集性大肠杆菌(EAEC)CVCC232噬菌体PNJ1809-11、PNJ1809-13的生物学特性,并对其作为环境消毒剂的杀菌效果进行评估。【方法】透射电镜下观察PNJ1809-11、PNJ1809-13的形态;通过宿主谱、最佳感染复数(MOI)、一步生长曲线、对pH和温度耐受性的测定分析PNJ1809-11、PNJ1809-13的生物学特性;比较2株噬菌体的体外杀菌效果和喷雾、雾化处理后的杀菌效果;检测噬菌体在模拟养殖环境下的耐受性,及喷雾处理的噬菌体制剂在模拟养殖环境下的杀菌效果和对宿主菌生物被膜的清除效果;分析宿主菌的抗性突变率。【结果】电镜下观察PNJ1809-11、PNJ1809-13均为肌尾病毒科噬菌体;PNJ1809-11可裂解155株大肠杆菌,PNJ1809-13可裂解46株大肠杆菌;噬菌体PNJ1809-11、PNJ1809-13的最佳感染复数(MOI)均为10,最适pH均为7;与PNJ1809-13相比,PNJ1809-11具有较好的热稳定性;在模拟的封闭装置中,将两株噬菌体分别经喷雾、雾化处理后,二者对宿主菌的杀灭效果均可达99%以上;对人工污染的粪便表面细菌的杀灭效果均达到99%以上。噬菌体PNJ1809-11、PNJ1809-13及两者的混合制剂(噬菌体鸡尾酒)对宿主菌CVCC232形成的生物被膜的裂解效率分别为78%、30%和83%。噬菌体PNJ1809-11在养殖温度、粪便pH以及阳光照射下,其耐受性均强于PNJ1809-13。宿主菌对PNJ1809-11、PNJ1809-13的抗性突变率分别为2.5×10^(-3)和1.0×10^(-3)。【结论】综上,噬菌体PNJ1809-11的环境耐受力更强,噬菌体鸡尾酒对生物被膜的裂解效果更好,提示噬菌体PNJ1809-13或噬菌体鸡尾酒经喷雾处理后具有作为环境杀菌剂的潜力。 展开更多
关键词 噬菌体 噬菌体鸡尾酒 消毒剂 耐受性 生物被膜
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Value of incorporating geospatial information into the prediction of on-street parking occupancy - A case study
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作者 Michael Balmer Robert Weibel haosheng huang 《Geo-Spatial Information Science》 SCIE EI CSCD 2021年第3期438-457,共20页
In light of growing urban traffic,car parking becomes increasingly critical for cities to manage.As a result,the prediction of parking occupancy has sparked significant research interest in recent years.While many ext... In light of growing urban traffic,car parking becomes increasingly critical for cities to manage.As a result,the prediction of parking occupancy has sparked significant research interest in recent years.While many external data sources have been considered in the prediction models,the underlying geographic context has mostly been ignored.Thus,in order to study the contribution of geospatial information to parking occupancy prediction models,road network centrality,land use,and Point of Interest(POI)data were incorporated in Random Forest(RF)and Artificial Neural Network(ANN,specifically Feedforward Neural Network FFNN)prediction models in this work.Model performances were compared to a baseline,which only considers historical and temporal input data.Moreover,the influence of the amount of training data,the prediction horizon,and the spatial variation of the prediction were explored.The results show that the inclusion of geospatial information led to a performance improvement of up to 25%compared to the baseline.Besides,as the prediction horizon expanded,predictions became less reliable,while the relevance of geospatial data increased.In general,land use and POI data proved to be more beneficial than road network centrality.The amount of training data did not have a significant influence on the performance of the RF model.The ANN model,conversely,achieved optimal results on a training input of 5 days.Likely attributable to varying occupancy patterns,prediction performance disparities could be identified for different parking districts and street segments.Generally,the RF model outperformed the ANN model on all predictions. 展开更多
关键词 Parking occupancy PREDICTION on-street parking geospatial information machine learning
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