Powdery mildew of wheat is a destructive disease seriously threatening yield and quality worldwide.Comprehensive dissection of new resistance-related loci/genes is necessary to control this disease.LS5082 is a Chinese...Powdery mildew of wheat is a destructive disease seriously threatening yield and quality worldwide.Comprehensive dissection of new resistance-related loci/genes is necessary to control this disease.LS5082 is a Chinese wheat breeding line with resistance to powdery mildew.Genetic analysis,using the populations of LS5082 and three susceptible parents(Shannong 29,Shimai 22 and Huixianhong),indicated that a single dominant gene,tentatively designated PmLS5082,conferred seedling resistance to different Blumeria graminis f.sp.tritici(Bgt)isolates.Bulked segregant RNA-Seq was carried out to map PmLS5082 and to profile differentially expressed genes associated with PmLS5082.PmLS5082 was mapped to a 0.7 cM genetic interval on chromosome arm 2BL,which was aligned to a 0.7 Mb physical interval of 710.3–711.0 Mb.PmLS5082 differs from the known powdery mildew(Pm)resistance genes on chromosome arm 2BL based on their origin,chromosome positions and/or resistance spectrum,suggesting PmLS5082 is most likely a new Pm gene/allele.Through clusters of orthologous groups and kyoto encyclopedia of genes and genomes analyses,differentially expressed genes(DEGs)associated with PmLS5082 were profiled.Six DEGs in the PmLS5082 interval were confirmed to be associated with PmLS5082 via qPCR analysis,using an additional set of wheat samples and time-course analysis postinoculation with Bgt isolate E09.Ten closely linked markers,including two kompetitive allele-specific PCR markers,were confirmed to be suitable for marker-assisted selection of PmLS5082 in different genetic backgrounds,thus can be used to detect PmLS5082 and pyramid it with other genes in breeding programs.展开更多
Wheat powdery mildew(Pm) is a major disease of wheat worldwide. During the past years, numerous studies have been published on molecular mapping of Pm resistance gene(s) in wheat. We summarized the relevant findings o...Wheat powdery mildew(Pm) is a major disease of wheat worldwide. During the past years, numerous studies have been published on molecular mapping of Pm resistance gene(s) in wheat. We summarized the relevant findings of 89 major resistance gene mapping studies and 25 quantitative trait loci(QTL) mapping studies. Major Pm resistance genes and QTLs were found on all wheat chromosomes, but the Pm resistance genes/QTLs were not randomly distributed on each chromosome of wheat. The summarized data showed that the A or B genome has more major Pm resistance genes than the D genome and chromosomes 1A, 2A, 2B, 5B, 5D, 6B, 7A and 7B harbor more major Pm resistance genes than the other chromosomes. For adult plant resistance(APR) genes/QTLs, B genome of wheat harbors more APR genes than A and D genomes, and chromosomes 2A, 4A, 5A, 1B, 2B, 3B, 5B, 6B, 7B, 2D, 5D and 7D harbor more Pm resistance QTLs than the other chromosomes,suggesting that A genome except 1A, 3A and 6A, B genome except 4B, D genome except 1D, 3D, 4D, and 6D play an important role in wheat combating against powdery mildew. Furthermore, Pm resistance genes are derived from wheat and its relatives, which suggested that the resistance sources are diverse and Pm resistance genes are diverse and useful in combating against the powdery mildew isolates. In this review, four APR genes, Pm38/Lr34/Yr18/Sr57, Pm46/Lr67/Yr46/Sr55, Pm?/Lr27/Yr30/Sr2 and Pm39/Lr46/Yr29, are not only resistant to powdery mildew but also effective for rust diseases in the field, indicating that such genes are stable and useful in wheat breeding programmes. The summarized data also provide chromosome locations or linked markers for Pm resistance genes/QTLs. Markers linked to these genes can also be utilized to pyramid diverse Pm resistance genes/QTLs more efficiently by marker-assisted selection.展开更多
The morphology,size,and distribution of Laves phases have important influences on the mechanical properties of laser-repaired Inconel 718(IN718)superalloy.Due to the deterioration of the substrate zone,the Laves phase...The morphology,size,and distribution of Laves phases have important influences on the mechanical properties of laser-repaired Inconel 718(IN718)superalloy.Due to the deterioration of the substrate zone,the Laves phase in the laser cladding zone of IN718 superalloy cannot be optimized by a hightemperature solution treatment.In this study,an in situ laser heat-treatment method was proposed to regulate the morphology and size of the Laves phase in the laser cladding zone of IN718 superalloy without impacting the substrate zone.In the in situ laser heat-treatment process,a laser was used to heat previously deposited layers with optimized manufacturing parameters.A thermocouple and an infrared camera were used to analyze thermal cycles and real-time temperature fields,respectively.Microstructures and micro-segregations were observed by optical microscopy,scanning electron microscopy,and electron probe microanalysis.It was found that the in situ laser heat treatment effectively changed the morphology and size of the Laves phase,which was transformed from a continuous striplike shape to a discrete granular shape.The effective temperature range and duration were the two main factors influencing the Laves phase during the in situ laser heat-treatment process.The effective temperature range was determined by the laser linear energy density,and the peak temperature increased with the increase of the linear energy density.In addition,the temperature amplitude could be reduced by simultaneously increasing the laser power and the scanning velocity.Finally,a flow diagram was developed based on the in situ laser heat-treatment process,and the deposition of a single-walled sample with fine and granular Laves phases was detected.展开更多
Solid-state sodium metal batteries utilizing inorganic solid electrolytes(SEs)hold immense potentials such as intrinsical safety,high energy density,and environmental sustainability.However,the interfacial inhomogenei...Solid-state sodium metal batteries utilizing inorganic solid electrolytes(SEs)hold immense potentials such as intrinsical safety,high energy density,and environmental sustainability.However,the interfacial inhomogeneity/instability at the anode-SE interface usually triggers the penetration of sodium dendrites into the electrolyte,leading to short circuit and battery failure.Herein,confronting with the original nonuniform and high-resistance solid electrolyte interphase(SEI)at the Na-Na_(3)Zr_(2)Si_(2)PO_(12)interface,an oxygen-regulated SEI innovative approach is proposed to enhance the cycling stability of anode-SEs interface,through a spontaneous reaction between the metallic sodium(containing trace amounts of oxygen)and the Na_(3)Zr_(2)Si_(2)POi_(2)SE.The oxygen-regulated spontaneous SEI is thin,uniform,and kinetically stable to facilitate homogenous interfacial Na^+transportation,Benefitting from the optimized SEI,the assembled symmetric cell exhibits an ultra-stable sodium plating/stripping cycle for over 6600 h under a practical capacity of 3 mAh cm^(-2).Qua si-sol id-state batteries with Na_(3)V_(2)(PO_(4))_(3)cathode deliver excellent cyclability over 500 cycles at a rate of 0.5 C(1 C=117 mA cm^(-2))with a high capacity retention of95.4%.This oxygen-regulated SEI strategy may offer a potential avenue for the future development of high-energy-density solid-state metal batteries.展开更多
Improved lodging resistance is important for achieving high yield in irrigated environments.This study was conducted to determine genotypic variation in lodging resistance and related morphological traits among winter...Improved lodging resistance is important for achieving high yield in irrigated environments.This study was conducted to determine genotypic variation in lodging resistance and related morphological traits among winter wheat cultivars planted at two densities,and to identify key traits associated with lodging resistance.Lodging performance of 28 genotypes,including 24 released cultivars and four advanced lines,was evaluated at 250 plants per square meter and 500 plants per square meter in Shandong province during the 2008–2009 and 2009–2010 crop seasons.At the higher density,the average grain yield was 2.6%higher,even though lodging score rose by as much as 136%.The higher planting density increased lodging through increased leaf area index(LAI),plant height,center of gravity and length of basal internodes,and reduced grain weight per spike and diameter of the lower two stem internodes.LAI,center of gravity and diameter of first internodes,as the important indicators for lodging resistance,were significantly correlated with lodging score,with R=0.62,0.59 and–0.52(P<0.01),respectively.Plant pushing resistance was significantly associated with diameter and length of the first internodes(R=0.71–0.77,P<0.01),indicating it could be used to assess the strength of the lower stem.Higher planting density could be used to select genotypes with lodging resistance in irrigated environments.Cultivars carrying high plant density tolerance and high yield potential,such as Jimai 22 and Liangxing 66,were recommended as leading cultivars for production as well as elite crossing parents for further increasing yield potential in the Yellow and Huai Valleys Winter Wheat Zone in China.展开更多
Spring radiation frost(SRF)is a severe environmental stress which impairs wheat yield and productivity worldwide.To better understand the mechanism of wheat(Triticum aestivum)responding to SRF,a comparative proteomic ...Spring radiation frost(SRF)is a severe environmental stress which impairs wheat yield and productivity worldwide.To better understand the mechanism of wheat(Triticum aestivum)responding to SRF,a comparative proteomic analysis was performed to analyze the changes of the key proteins in two wheat cultivars Jimai22 and Luyuan301 with high and low tolerance to SRF respectively.A total of 43 differentially expressed proteins(DEPs)which mainly involved in carbohydrate metabolism,amino acid metabolism,resistance proteins and antioxidant enzymes,photosynthesis and cellular respiration proteins,cell-wall related proteins,protein translation/processing/degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOF MS.The results revealed that of the 21 DEPs in Jimai22 responding to the SRF,13 DEPs were upregulated and 8 DEPs were downregulated,and that of the 22 DEPs in Luyuan301,9 DEPs were upregulated and 13 DEPs were downregulated.These DEPs might be responsible for the stronger cold resistance of Jimai22 compared to Luyuan301.The expression pattern and function analysis of these DEPs were very significant to understanding the mechanism of the SRF responses in wheat.展开更多
基金financially supported by ‘‘Double Hundred” Plan for Foreign Experts in Shandong Province, Chinafinancially supported by the National Natural Science Foundation of China (32072053, 31971874, and 32171990)+4 种基金Taishan Scholars Project (tsqn201812123)Key Research and Development Program of Shandong Province (2020CXGC010805)Open Project Funding of the State Key Laboratory of Crop Stress Adaptation and Improvement (CX1130A0920014)State Key Laboratory of Plant Cell and Chromosome Engineering (PCCE-KF-2019-04)Iran National Science Foundation (INSF) Grant 99014038
文摘Powdery mildew of wheat is a destructive disease seriously threatening yield and quality worldwide.Comprehensive dissection of new resistance-related loci/genes is necessary to control this disease.LS5082 is a Chinese wheat breeding line with resistance to powdery mildew.Genetic analysis,using the populations of LS5082 and three susceptible parents(Shannong 29,Shimai 22 and Huixianhong),indicated that a single dominant gene,tentatively designated PmLS5082,conferred seedling resistance to different Blumeria graminis f.sp.tritici(Bgt)isolates.Bulked segregant RNA-Seq was carried out to map PmLS5082 and to profile differentially expressed genes associated with PmLS5082.PmLS5082 was mapped to a 0.7 cM genetic interval on chromosome arm 2BL,which was aligned to a 0.7 Mb physical interval of 710.3–711.0 Mb.PmLS5082 differs from the known powdery mildew(Pm)resistance genes on chromosome arm 2BL based on their origin,chromosome positions and/or resistance spectrum,suggesting PmLS5082 is most likely a new Pm gene/allele.Through clusters of orthologous groups and kyoto encyclopedia of genes and genomes analyses,differentially expressed genes(DEGs)associated with PmLS5082 were profiled.Six DEGs in the PmLS5082 interval were confirmed to be associated with PmLS5082 via qPCR analysis,using an additional set of wheat samples and time-course analysis postinoculation with Bgt isolate E09.Ten closely linked markers,including two kompetitive allele-specific PCR markers,were confirmed to be suitable for marker-assisted selection of PmLS5082 in different genetic backgrounds,thus can be used to detect PmLS5082 and pyramid it with other genes in breeding programs.
基金Supported by the NSF of China(Grant no.31471488)State Key Laboratory of Crop Biology(2017KF03)+3 种基金Shandong Province Key Technology Innovation Project(2014GJJS0201-1)Transgenic Special Item(2016ZX08002003)National Modern Agricultural Industry System Construction Project(CARS-03-1-8)The Scholars of Taishan Seed Industry Project(2014-2019)
文摘Wheat powdery mildew(Pm) is a major disease of wheat worldwide. During the past years, numerous studies have been published on molecular mapping of Pm resistance gene(s) in wheat. We summarized the relevant findings of 89 major resistance gene mapping studies and 25 quantitative trait loci(QTL) mapping studies. Major Pm resistance genes and QTLs were found on all wheat chromosomes, but the Pm resistance genes/QTLs were not randomly distributed on each chromosome of wheat. The summarized data showed that the A or B genome has more major Pm resistance genes than the D genome and chromosomes 1A, 2A, 2B, 5B, 5D, 6B, 7A and 7B harbor more major Pm resistance genes than the other chromosomes. For adult plant resistance(APR) genes/QTLs, B genome of wheat harbors more APR genes than A and D genomes, and chromosomes 2A, 4A, 5A, 1B, 2B, 3B, 5B, 6B, 7B, 2D, 5D and 7D harbor more Pm resistance QTLs than the other chromosomes,suggesting that A genome except 1A, 3A and 6A, B genome except 4B, D genome except 1D, 3D, 4D, and 6D play an important role in wheat combating against powdery mildew. Furthermore, Pm resistance genes are derived from wheat and its relatives, which suggested that the resistance sources are diverse and Pm resistance genes are diverse and useful in combating against the powdery mildew isolates. In this review, four APR genes, Pm38/Lr34/Yr18/Sr57, Pm46/Lr67/Yr46/Sr55, Pm?/Lr27/Yr30/Sr2 and Pm39/Lr46/Yr29, are not only resistant to powdery mildew but also effective for rust diseases in the field, indicating that such genes are stable and useful in wheat breeding programmes. The summarized data also provide chromosome locations or linked markers for Pm resistance genes/QTLs. Markers linked to these genes can also be utilized to pyramid diverse Pm resistance genes/QTLs more efficiently by marker-assisted selection.
基金supported by Aero Engine Corporation of China Xi’an Aero-Engine Ltd.(N2018KD040252)the fund of the State Key Laboratory of Solidification Processing in Northwestern Polytechnical University(2020-TS-03)。
文摘The morphology,size,and distribution of Laves phases have important influences on the mechanical properties of laser-repaired Inconel 718(IN718)superalloy.Due to the deterioration of the substrate zone,the Laves phase in the laser cladding zone of IN718 superalloy cannot be optimized by a hightemperature solution treatment.In this study,an in situ laser heat-treatment method was proposed to regulate the morphology and size of the Laves phase in the laser cladding zone of IN718 superalloy without impacting the substrate zone.In the in situ laser heat-treatment process,a laser was used to heat previously deposited layers with optimized manufacturing parameters.A thermocouple and an infrared camera were used to analyze thermal cycles and real-time temperature fields,respectively.Microstructures and micro-segregations were observed by optical microscopy,scanning electron microscopy,and electron probe microanalysis.It was found that the in situ laser heat treatment effectively changed the morphology and size of the Laves phase,which was transformed from a continuous striplike shape to a discrete granular shape.The effective temperature range and duration were the two main factors influencing the Laves phase during the in situ laser heat-treatment process.The effective temperature range was determined by the laser linear energy density,and the peak temperature increased with the increase of the linear energy density.In addition,the temperature amplitude could be reduced by simultaneously increasing the laser power and the scanning velocity.Finally,a flow diagram was developed based on the in situ laser heat-treatment process,and the deposition of a single-walled sample with fine and granular Laves phases was detected.
基金Zhejiang Provincial Natural Science Foundation of China(LZ23B030003)the National Key R&D Program(2022YFB2502000)+1 种基金the National Key R&D Program(2022YFB2502000)the Fundamental Research Funds for the Central Universities(2021FZZX001-09)。
文摘Solid-state sodium metal batteries utilizing inorganic solid electrolytes(SEs)hold immense potentials such as intrinsical safety,high energy density,and environmental sustainability.However,the interfacial inhomogeneity/instability at the anode-SE interface usually triggers the penetration of sodium dendrites into the electrolyte,leading to short circuit and battery failure.Herein,confronting with the original nonuniform and high-resistance solid electrolyte interphase(SEI)at the Na-Na_(3)Zr_(2)Si_(2)PO_(12)interface,an oxygen-regulated SEI innovative approach is proposed to enhance the cycling stability of anode-SEs interface,through a spontaneous reaction between the metallic sodium(containing trace amounts of oxygen)and the Na_(3)Zr_(2)Si_(2)POi_(2)SE.The oxygen-regulated spontaneous SEI is thin,uniform,and kinetically stable to facilitate homogenous interfacial Na^+transportation,Benefitting from the optimized SEI,the assembled symmetric cell exhibits an ultra-stable sodium plating/stripping cycle for over 6600 h under a practical capacity of 3 mAh cm^(-2).Qua si-sol id-state batteries with Na_(3)V_(2)(PO_(4))_(3)cathode deliver excellent cyclability over 500 cycles at a rate of 0.5 C(1 C=117 mA cm^(-2))with a high capacity retention of95.4%.This oxygen-regulated SEI strategy may offer a potential avenue for the future development of high-energy-density solid-state metal batteries.
基金the National Natural Science Foundation of China(31161140346)the State Key Laboratory of Crop Biology(2014KF02)the State Key Laboratory of Crop Stress Biology in Arid Areas.
文摘Improved lodging resistance is important for achieving high yield in irrigated environments.This study was conducted to determine genotypic variation in lodging resistance and related morphological traits among winter wheat cultivars planted at two densities,and to identify key traits associated with lodging resistance.Lodging performance of 28 genotypes,including 24 released cultivars and four advanced lines,was evaluated at 250 plants per square meter and 500 plants per square meter in Shandong province during the 2008–2009 and 2009–2010 crop seasons.At the higher density,the average grain yield was 2.6%higher,even though lodging score rose by as much as 136%.The higher planting density increased lodging through increased leaf area index(LAI),plant height,center of gravity and length of basal internodes,and reduced grain weight per spike and diameter of the lower two stem internodes.LAI,center of gravity and diameter of first internodes,as the important indicators for lodging resistance,were significantly correlated with lodging score,with R=0.62,0.59 and–0.52(P<0.01),respectively.Plant pushing resistance was significantly associated with diameter and length of the first internodes(R=0.71–0.77,P<0.01),indicating it could be used to assess the strength of the lower stem.Higher planting density could be used to select genotypes with lodging resistance in irrigated environments.Cultivars carrying high plant density tolerance and high yield potential,such as Jimai 22 and Liangxing 66,were recommended as leading cultivars for production as well as elite crossing parents for further increasing yield potential in the Yellow and Huai Valleys Winter Wheat Zone in China.
基金supported by Science&Technology Development Plan of Shandong Province(2013GNC11025)Shandong Agriculture and Seed Industry(2012)+3 种基金Funding for the Post-doctoral Innovative Projects of Shandong Province(201203024)the National Transgenic Major Project(2013ZX08002-004)China Agriculture Research System(CARS-03-1-08)Shandong Agriculture Research System,the national key technology R&D program of China(2011BAD35B03).
文摘Spring radiation frost(SRF)is a severe environmental stress which impairs wheat yield and productivity worldwide.To better understand the mechanism of wheat(Triticum aestivum)responding to SRF,a comparative proteomic analysis was performed to analyze the changes of the key proteins in two wheat cultivars Jimai22 and Luyuan301 with high and low tolerance to SRF respectively.A total of 43 differentially expressed proteins(DEPs)which mainly involved in carbohydrate metabolism,amino acid metabolism,resistance proteins and antioxidant enzymes,photosynthesis and cellular respiration proteins,cell-wall related proteins,protein translation/processing/degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOF MS.The results revealed that of the 21 DEPs in Jimai22 responding to the SRF,13 DEPs were upregulated and 8 DEPs were downregulated,and that of the 22 DEPs in Luyuan301,9 DEPs were upregulated and 13 DEPs were downregulated.These DEPs might be responsible for the stronger cold resistance of Jimai22 compared to Luyuan301.The expression pattern and function analysis of these DEPs were very significant to understanding the mechanism of the SRF responses in wheat.