Bisphenol A (BPA) was used in the plastic industry and widely distributed in the environment, which might cause adverse effects to living organisms. In order to study the effects of BPA on the organs of mice brain, li...Bisphenol A (BPA) was used in the plastic industry and widely distributed in the environment, which might cause adverse effects to living organisms. In order to study the effects of BPA on the organs of mice brain, liver and testical, the KM mice were administrated to different concentrations of BPA (0, 0.09, 0.9, 9 mg/kg·d). After 14 days, the reactive oxygen species (ROS), malondialdehyde (MDA) and glutathione (GSH?concentrations in the three organs were measured. Results showed that the ROS and MDA contents were increased with the added concentration of the BPA. But the GSH content was decreased. It is indicated BPA can induce the mice’s oxidative damage and the effect is much easier for the brain.展开更多
Plastics such as polyvinyl chlorides （PVC） are widely used in many indoor constructed environments; however, their unbound chemicals, such as di-（2-ethylhexyl） phthalates （DEHP）, can leach into the surrounding e...Plastics such as polyvinyl chlorides （PVC） are widely used in many indoor constructed environments; however, their unbound chemicals, such as di-（2-ethylhexyl） phthalates （DEHP）, can leach into the surrounding environment. This study focused on DEHP＇s effect on the central nervous system by determining the precise DEHP content in mice brain tissue after exposure to the chemical, to evaluate the specific exposure range. Primary neuronal-astrocyte co-culture systems were used as in vitro models for chemical hazard identification of DEHP. Oxidative stress was hypothesized as a probable mechanism involved, and therefore the total reactive oxygen species （ROS） concentration was determined as a biomarker of oxidative stress. In addition, NeuriteTracer, a neurite tracing plugin with ImageJ, was used to develop an assay for neurotoxicity to provide quantitative measurements of neurological parameters, such as neuronal number, neuron count and neurite length, all of which could indicate neurotoxic effects. The results showed that with 1 nmol/L DEHP exposure, there was a significant increase in ROS concentrations, indicating that the neuronal-astrocyte cultures were injured due to exposure to DEHP. In response, astrocyte proliferation （gliosis） was initiated, serving as a mechanism to maintain a homeostatic environment for neurons and protect neurons from toxic chemicals. There is a need to assess the cumulative effects of DEHP in animals to evaluate the possible uotake and effects on the human neuronal system from exoosure to DEHP in the indoor environment.展开更多
文摘Bisphenol A (BPA) was used in the plastic industry and widely distributed in the environment, which might cause adverse effects to living organisms. In order to study the effects of BPA on the organs of mice brain, liver and testical, the KM mice were administrated to different concentrations of BPA (0, 0.09, 0.9, 9 mg/kg·d). After 14 days, the reactive oxygen species (ROS), malondialdehyde (MDA) and glutathione (GSH?concentrations in the three organs were measured. Results showed that the ROS and MDA contents were increased with the added concentration of the BPA. But the GSH content was decreased. It is indicated BPA can induce the mice’s oxidative damage and the effect is much easier for the brain.
基金supported by the Key Project of National Natural Science Foundation of China(No.51136002)China Key Technologies R&D Program(No.2012BAJ02B03)
文摘Plastics such as polyvinyl chlorides （PVC） are widely used in many indoor constructed environments; however, their unbound chemicals, such as di-（2-ethylhexyl） phthalates （DEHP）, can leach into the surrounding environment. This study focused on DEHP＇s effect on the central nervous system by determining the precise DEHP content in mice brain tissue after exposure to the chemical, to evaluate the specific exposure range. Primary neuronal-astrocyte co-culture systems were used as in vitro models for chemical hazard identification of DEHP. Oxidative stress was hypothesized as a probable mechanism involved, and therefore the total reactive oxygen species （ROS） concentration was determined as a biomarker of oxidative stress. In addition, NeuriteTracer, a neurite tracing plugin with ImageJ, was used to develop an assay for neurotoxicity to provide quantitative measurements of neurological parameters, such as neuronal number, neuron count and neurite length, all of which could indicate neurotoxic effects. The results showed that with 1 nmol/L DEHP exposure, there was a significant increase in ROS concentrations, indicating that the neuronal-astrocyte cultures were injured due to exposure to DEHP. In response, astrocyte proliferation （gliosis） was initiated, serving as a mechanism to maintain a homeostatic environment for neurons and protect neurons from toxic chemicals. There is a need to assess the cumulative effects of DEHP in animals to evaluate the possible uotake and effects on the human neuronal system from exoosure to DEHP in the indoor environment.
