LncRNA MIR210HG对膀胱癌细胞恶性生物学行为的影响

目的:分析长链非编码RNA(long non-coding RNA,LncRNA)MIR210HG与膀胱癌患者临床病理特征的关系,探究其对膀胱癌恶性生物学行为的影响。方法:收集2022年6月至2022年12月河北医科大学第四医院70例膀胱癌患者的临床病理资料,使用实时荧光定量PCR(RT-PCR)检测LncRNA MIR210HG表达,分析其与临床病理特征的相关性。采用MTS实验、克隆形成实验、划痕实验、Transwell侵袭实验研究LncRNA MIR210HG对膀胱癌细胞生物学行为的影响。结果:膀胱癌组织及膀胱癌细胞T24、5637和SW780中LncRNA MIR210HG表达量较高(均P<0.05)。发生淋巴结转移的膀胱癌组织中的LncRNA MIR210HG相对表达量为3.847±1.047,高于未发生淋巴结转移的膀胱癌组织中的2.915±0.904;肌层浸润的膀胱癌组织中的LncRNA MIR210HG相对表达量为3.353±1.032,高于非肌层浸润的膀胱癌组织中的2.822±0.872;两者比较差异均具有统计学意义(均P<0.05)。下调LncRNA MIR210HG的表达可抑制膀胱癌细胞增殖、迁移及侵袭的能力(均P<0.05),过表达LncRNA MIR210HG可提高膀胱癌细胞增殖、迁移及侵袭的能力(均P<0.05)。结论:LncRNA MIR210HG在膀胱癌组织中明显高表达,可促进膀胱癌肌层浸润及淋巴结转移,并可为膀胱癌患者的临床治疗及提高其总生存率提供参考依据。

In situ construction of Co/N/C-based heterojunction on biomass-derived hierarchical porous carbon with stable active sites using a Co-N protective strategy for high-efficiency ORR,OER and HER trifunctional electrocatalysts

The facile designs and fabrication of noble metal-free electrocatalysts are highly required to achieve multifunctional catalytic activity with excellent stability in Zn-air batteries,fuel cells and water splitting systems.Herein,a heterostructure engineering is applied to construct the high performance Co,Ncontaining carbon-based multifunctional electrocatalysts with the feature of isotype(i.e.n-n type Co_(2)N_(0.67)-BHPC)and anisotype(i.e.p-n type Co_(2)O_(3)-BHPC)heterojunctions for ORR,OER and HER.The nn type Co_(2)N_(0.67)-BHPC,in which biomass(e.g.mushroom)-derived hierarchical porous carbon(BHPC)incorporated with nonstoichiometric active species Co_(2)N_(0.67),is fabricated by using an in situ protective strategy of macrocyclic central Co-N_(4) from CoTPP(5,10,15,20-tetrakis(phenyl)porphyrinato cobalt)precursor through the intermolecularπ-πinteractions between CoTPP and its metal-free analogue H_(2) TPP.Meanwhile,an unprotected strategy of macrocyclic central Co-N_(4) from CoTPP can afford the anisotype Co_(2)O_(3)-BHPC p-n heterojunction.The as-prepared n-n type Co_(2)N_(0.67)-BHPC heterojunction exhibited a higher density of Co-based active sites with outstanding stability and more efficient charge transfer at the isotype heterojunction interface in comparison with p-n type Co_(2)O_(3)-BHPC heterojunction.Consequently,for ORR,Co_(2)N_(0.67)-BHPC exhibits the more positive onset and half-wave potentials of 0.93 and 0.86 V vs.RHE,respectively,superior to those of the commercial 20 wt%Pt/C and most of Cobased catalysts reported so far.To drive a current density of 10 mA cm^(-2),Co_(2)N_(0.67)-BHPC also shows the lower overpotentials of 0.34 and 0.21 V vs.RHE for OER and HER,respectively.Furthermore,the Zn-air battery equipped with Co_(2)N_(0.67)-BHPC displays higher maximum power density(109 mW cm^(-2))and charge-discharge cycle stability.Interestingly,the anisotype heterojunction Co_(2)O_(3)-BHPC as trifunctional electrocatalyst reveals evidently photoelectrochemical enhancement compared with the photostable Co_(2)N_(0.67)-BHPC.That is to say,isotype heterojunction material(n-n type Co^(2)N_(0.67)-BHPC)is equipped with better electrocatalytic performance than anisotype one(p-n type Co_(2)O_(3)-BHPC),but the opposite is true in photoelectrochemical catalysis.Meanwhile,the possible mechanism is proposed based on the energy band structures of the Co_(2)N_(0.67)-BHPC and Co_(2)O_(3)-BHPC and the cocatalyst effects.The present work provides much more possibilities to tune the electrocatalytic and photoelectrochemical properties of catalysts through a facile combination of heterostructure engineering protocol and macrocyclic central metal protective strategy.

Electrifying the future:the advances and opportunities of electrocatalytic carbon dioxide reduction in acid

Transforming carbon dioxide(CO_(2))into products using renewable electricity is a crucial and captivating quest for a green and circular economy.Compared with commonly used alkali electrolytes,acidic media for electrocatalytic CO_(2) reduction(CO_(2)RR)boasts several advantages,such as high carbon utilization efficiency,high overall energy utilization rate,and low carbonate formation,making it a compelling choice for industrial applications.However,the acidic CO_(2)RR also struggles with formidable hurdles,encompassing the fierce competition with the hydrogen evolution reaction,the low CO_(2) solubility and availability,and the suboptimal performance of catalysts.This review provides a comprehensive overview of the CO_(2)RR in acidic media.By elucidating the underlying regulatory mechanism,we gain valuable insights into the fundamental principles governing the acidic CO_(2)RR.Furthermore,we examine cutting-edge strategies aimed at optimizing its performance and the roles of reactor engineering,especially membrane electrode assembly reactors,in facilitating scalable and carbon efficient conversion.Moreover,we present a forward-looking perspective,highlighting the promising prospects of acidic CO_(2)RR research in ushering us towards a carbon-neutral society.

Hydrogen-bond locked purine chromophores with high photostability for lipid droplets imaging in cells and tissues

Recently, hydrogen-bonding has attracted extensive attention in the design of chromophores. Here, a new class of hydrogen-bond locked purine chromophores(HOPs) were reported by introducing a hydroxyphenyl group into the C(6) position of purine. The intramolecular hydrogen bond plays a dominant role to light up these probes. As a bonus, HOPs show high photostability. Moreover, HOPs exhibit remarkable capability for the specific lipid droplets imaging in living cells with excellent biocompatibility and are also potential for diagnosing fatty liver diseases. These results bring important new insights into the photophysics of the purine-based chromophores and provide a new scaffold with high photostability for bioimaging.

Synthesis of 2-Trifluoromethyl-4-aminoquinolines via Heating-Promoted Multi-component Reaction of CF_(3)-Imidoyl Sulfoxonium Ylides and Amines with Difluorocarbene as a C1 Synthon

A thermally-induced multi-component reaction of CF_(3)-substituted imidoyl sulfoxonium ylides(TFISYs),amines and(triphenylphosphonio)difluoroacetate(PDFA)has been developed,allowing a facile access to 2-trifluoromethyl-4-aminoquinolines in high yields.The reaction proceeds smoothly with or without the addition of sulfur and utilizes difluorocarbene as a C1 synthon under simply heating conditions.Mechanistic study reveals that in-situ generated thiocarbonyl fluoride,isothiocyanate or gem-difluoroalkene might act as the key reaction intermediates.

Additive Manufacturing of Liquid-Cooled Ceramic Heat Sinks:An Experimental and Numerical Study

With recent advances in power electronic packaging technologies,liquid-cooled ceramic heat sinks have been considered as a promising solution for further improving the performance of power electronic devices.In this study,several aluminum oxide heat sinks were fabricated and tested using the digital light processing-based ad-ditive manufacturing method,to verify their practical performance.The results showed that the complex cooling structures inside the heat sinks can be completely formed and exhibited high surface quality.The experimental thermal and hydraulic performances of the heat sinks were consistent with the numerically modeled predictions.Furthermore,by exploiting the advantages of additive manufacturing,a direct manifold microchannel(MMC)configuration was designed to reduce the vertical flow of the traditional MMC configuration and achieve an im-proved cooling efficiency.At a constant volumetric flow rate of 1 L/min,the direct MMC configuration achieved a 19.8%reduction in pressure drop and an 11.8%reduction in thermal resistance,as well as a more uniform temperature distribution.