The method of transforming resistance gene to carbendazim into Trichoderma sp. was studied by using genetic engineering technique. The results show that the time of fungicide selection on the transformed Trichoderma s...The method of transforming resistance gene to carbendazim into Trichoderma sp. was studied by using genetic engineering technique. The results show that the time of fungicide selection on the transformed Trichoderma sp. may have very important effect on the resistance gene transformation. By this method, the resistance gene to carbendazim was transformed into Trichoderma harzianum, a strain of T. harzianum with resistance to carbendazim was achieved. The transformant could grow on the medium containing 150μg/mL carbendazim. The resistance is stable after 10 times transfer on non-selective medium.展开更多
文摘The method of transforming resistance gene to carbendazim into Trichoderma sp. was studied by using genetic engineering technique. The results show that the time of fungicide selection on the transformed Trichoderma sp. may have very important effect on the resistance gene transformation. By this method, the resistance gene to carbendazim was transformed into Trichoderma harzianum, a strain of T. harzianum with resistance to carbendazim was achieved. The transformant could grow on the medium containing 150μg/mL carbendazim. The resistance is stable after 10 times transfer on non-selective medium.
