Hepatic GCK is a key enzyme in glucose homeostasis and, as such, is a potential target for treatment strategies of diabetes. We investigated the effect of Persian shallot (Allium hirtifolium Boiss) hydroalchoholic ext...Hepatic GCK is a key enzyme in glucose homeostasis and, as such, is a potential target for treatment strategies of diabetes. We investigated the effect of Persian shallot (Allium hirtifolium Boiss) hydroalchoholic extract on blood glucose level, plasma insulin level, GCK activity and its gene expression. Thirty two male rats were divided into 4 groups of 8, diabetic groups received 100 and 200 mg/kg Persian shallot extract, diabetic control and normal control received 0.9% saline for 30 days. Investigations of gene expression by Real-Time PCR showed that Persian shallot had led to gently increased GCK gene expression in diabetic rats. GCK activity increased significantly in Persian shallot treated group in dose dependent manner (P < 0.05). These results indicated that Persian shallot exhibited a significant potential as a hypoglycemic agent perhaps via its ability to enhance insulin secretion, GCK gene expression and its activity.展开更多
In vitro maturation(IVM)is considered a potential assisted reproductive technology that is a safer and simpler alternative to conventional in vitro fertilization.It is primarily used in patients with impaired oocyte m...In vitro maturation(IVM)is considered a potential assisted reproductive technology that is a safer and simpler alternative to conventional in vitro fertilization.It is primarily used in patients with impaired oocyte maturation and for the treatment of infertile women who are at risk of fertility loss.In addition,IVM is currently used mainly in polycystic ovarian syndrome patients with a high ovarian response and is stll considered an experimental option in fertility preservation.Producing highly competent oocytes during IVM is considered a key step in the success of in vitro production(IVP)of embryos.Some factors,such as culture medium conditions and other supplements,have a significant impact on oocyte IVM performance.One of the known disruptors of oocyte developmental competence in IVP is oxidative stress(Os),which is caused by an imbalance between the production and neutralization of reactive oxygen species(ROS).In vitro conditions induce supraphysiological ROS levels due to exposure to an oxidative environment and the isolation of the oocyte from the follicle protective antioxidant milieu.Given the importance of Os in oocyte competence,the establishment of standardized antioxidant IVM systems is critical for improving the overall success of IVP.This review focuses on the main antioxidants tested to protect oocytes against OS in IVM.展开更多
文摘Hepatic GCK is a key enzyme in glucose homeostasis and, as such, is a potential target for treatment strategies of diabetes. We investigated the effect of Persian shallot (Allium hirtifolium Boiss) hydroalchoholic extract on blood glucose level, plasma insulin level, GCK activity and its gene expression. Thirty two male rats were divided into 4 groups of 8, diabetic groups received 100 and 200 mg/kg Persian shallot extract, diabetic control and normal control received 0.9% saline for 30 days. Investigations of gene expression by Real-Time PCR showed that Persian shallot had led to gently increased GCK gene expression in diabetic rats. GCK activity increased significantly in Persian shallot treated group in dose dependent manner (P < 0.05). These results indicated that Persian shallot exhibited a significant potential as a hypoglycemic agent perhaps via its ability to enhance insulin secretion, GCK gene expression and its activity.
基金financially supported by the Abadan University of Medical Sciences,Abadan,Iran(1401U-1467).
文摘In vitro maturation(IVM)is considered a potential assisted reproductive technology that is a safer and simpler alternative to conventional in vitro fertilization.It is primarily used in patients with impaired oocyte maturation and for the treatment of infertile women who are at risk of fertility loss.In addition,IVM is currently used mainly in polycystic ovarian syndrome patients with a high ovarian response and is stll considered an experimental option in fertility preservation.Producing highly competent oocytes during IVM is considered a key step in the success of in vitro production(IVP)of embryos.Some factors,such as culture medium conditions and other supplements,have a significant impact on oocyte IVM performance.One of the known disruptors of oocyte developmental competence in IVP is oxidative stress(Os),which is caused by an imbalance between the production and neutralization of reactive oxygen species(ROS).In vitro conditions induce supraphysiological ROS levels due to exposure to an oxidative environment and the isolation of the oocyte from the follicle protective antioxidant milieu.Given the importance of Os in oocyte competence,the establishment of standardized antioxidant IVM systems is critical for improving the overall success of IVP.This review focuses on the main antioxidants tested to protect oocytes against OS in IVM.
