Objective:To sereen methanol and dichloromethane extracts of stem bark of Pterocarpus erinaceus for anti-inflammatory,analgesic.in vitro antioxidant activities and phytochemical analysis.Methods:Anti-inflammatory acti...Objective:To sereen methanol and dichloromethane extracts of stem bark of Pterocarpus erinaceus for anti-inflammatory,analgesic.in vitro antioxidant activities and phytochemical analysis.Methods:Anti-inflammatory activity was detcrmined by using carrageenan inducededema of mice paw and croton oil-induced edema of mice ear:analgesic effect was evaluated using acetic acid-induced writhing.Phytochemical screening of extracts was performed by thin layer chromatography.The chromatographic fractionation led to the isolation of main active components as friedelin.lupeol and epicathcchin.The structures were established hy TLC and nuclear magnetic resonance studies.Results:Both methanol and dichloromethane extracts, triedelin.lupeol and epicatechin showed a significant anti—inflammatory effect using croton oil induced—ear edema.Furthermore,the action of dichloroinethane extract was more important. At the doses of 100 and 200 mg/kg.the methanol extract was able to reduce the carrageeuan induced-hind paw edema,while al the doses of 100.200 and 400 mg/kg.it showed an important analgesic effect against writhing induced by acetic acid injection of 38.8%.68.0%and 74.3%, respectively.Antioxidative properties of methanol extract and its dichloromethane and ethyl acetate fractions were assessed by using the 1,1—diphenyl—2—picrylhydrazyl method.The methanol extract showed the stronger radical scavenging activity than dichloromethane and ethyl acetate fractions,with an antiradical power of 5.3.5 and 2 respectively.The main components isolated from these extracts as friedelin,lupeol and epicathechin were responsible of these activities.Conclusions:The results suggest that the stem hark extracts of Pterocarpus erinaceus possessed important anti-inflammatory.analgesie activities and strong antioxidant properties, therefore,they could he used as natural potential ingredients for pharma ceutical industry.展开更多
文摘Objective:To sereen methanol and dichloromethane extracts of stem bark of Pterocarpus erinaceus for anti-inflammatory,analgesic.in vitro antioxidant activities and phytochemical analysis.Methods:Anti-inflammatory activity was detcrmined by using carrageenan inducededema of mice paw and croton oil-induced edema of mice ear:analgesic effect was evaluated using acetic acid-induced writhing.Phytochemical screening of extracts was performed by thin layer chromatography.The chromatographic fractionation led to the isolation of main active components as friedelin.lupeol and epicathcchin.The structures were established hy TLC and nuclear magnetic resonance studies.Results:Both methanol and dichloromethane extracts, triedelin.lupeol and epicatechin showed a significant anti—inflammatory effect using croton oil induced—ear edema.Furthermore,the action of dichloroinethane extract was more important. At the doses of 100 and 200 mg/kg.the methanol extract was able to reduce the carrageeuan induced-hind paw edema,while al the doses of 100.200 and 400 mg/kg.it showed an important analgesic effect against writhing induced by acetic acid injection of 38.8%.68.0%and 74.3%, respectively.Antioxidative properties of methanol extract and its dichloromethane and ethyl acetate fractions were assessed by using the 1,1—diphenyl—2—picrylhydrazyl method.The methanol extract showed the stronger radical scavenging activity than dichloromethane and ethyl acetate fractions,with an antiradical power of 5.3.5 and 2 respectively.The main components isolated from these extracts as friedelin,lupeol and epicathechin were responsible of these activities.Conclusions:The results suggest that the stem hark extracts of Pterocarpus erinaceus possessed important anti-inflammatory.analgesie activities and strong antioxidant properties, therefore,they could he used as natural potential ingredients for pharma ceutical industry.
