The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lip...The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.展开更多
In healthy subjects, the rate of acid reflux during transient lower esophageal sphincter relaxations (TLESRs) is more frequent at the proximal margin of the L ES. In this study, we investigated the hypothesis that the...In healthy subjects, the rate of acid reflux during transient lower esophageal sphincter relaxations (TLESRs) is more frequent at the proximal margin of the L ES. In this study, we investigated the hypothesis that the rate of acid reflux a t the proximal margin of LES during TLESRs is similar in reflux disease to that in healthy subjects. Concurrent esophageal manometry and pH monitoring were perf ormed in the sitting position for 3 hr after a standard meal in 10 patients with reflux disease and 10 agematched healthy controls. The rate of TLESRs in patien ts with reflux disease (5.0/hr [3.3-6.7]; median [interquartile range])-was si milar to that of controls (4.5/hr [3.7-5.7]). The incidence of acid reflux 7 cm above the LES during TLESRs in patients (48.1%[27.2-71.4%])was significantly higher than that in controls (10.9%[0.0-18.8%]) but there was no difference 2 cm above the LES (75.0 [69.2-87.5] vs. 74.3 [55.5-90.0%]). The rate of TLES Rs and the incidence of acid reflux during TLESRs are not increased in reflux disease. These findings, therefore, indicat e that reflux disease is not a disorder of TLESRs and relates more to the proxim al extent of the refluxate.展开更多
基金National Natural Science Foundation of China(No.29876031)
文摘The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.
文摘In healthy subjects, the rate of acid reflux during transient lower esophageal sphincter relaxations (TLESRs) is more frequent at the proximal margin of the L ES. In this study, we investigated the hypothesis that the rate of acid reflux a t the proximal margin of LES during TLESRs is similar in reflux disease to that in healthy subjects. Concurrent esophageal manometry and pH monitoring were perf ormed in the sitting position for 3 hr after a standard meal in 10 patients with reflux disease and 10 agematched healthy controls. The rate of TLESRs in patien ts with reflux disease (5.0/hr [3.3-6.7]; median [interquartile range])-was si milar to that of controls (4.5/hr [3.7-5.7]). The incidence of acid reflux 7 cm above the LES during TLESRs in patients (48.1%[27.2-71.4%])was significantly higher than that in controls (10.9%[0.0-18.8%]) but there was no difference 2 cm above the LES (75.0 [69.2-87.5] vs. 74.3 [55.5-90.0%]). The rate of TLES Rs and the incidence of acid reflux during TLESRs are not increased in reflux disease. These findings, therefore, indicat e that reflux disease is not a disorder of TLESRs and relates more to the proxim al extent of the refluxate.
