The effect of the chelating agent 4,7-bis（chlorosulfophenyl）-1,10-phenanthroline-2,9-dicarboxylic acid （BCPDA） labeled hepatitis B surface antibodies（HBsAb） on time-resolved fluoroimmunoassays（TRFIA） was inves...The effect of the chelating agent 4,7-bis（chlorosulfophenyl）-1,10-phenanthroline-2,9-dicarboxylic acid （BCPDA） labeled hepatitis B surface antibodies（HBsAb） on time-resolved fluoroimmunoassays（TRFIA） was investigated. The labeling detection method was established. The biotin-streptavidin（BAS） system was introduced, and the multi-stage amplification effect of the BAS system was analyzed. It is found that the BCPDA-Eu^3＋-HBsAb-BAS fluorescent marker can emit strong fluorescence. Compared to BCPDA-Eu^3＋-HBsAb, BCPDA-Eu^3＋-HBsAb-BAS demonstrates an enhanced fluorescence intensity by over 10 times. This study provides a guidance for the next generation non-radio immunoassays in clinical diagnosis,展开更多
Since Global Navigation Satellite System（GNSS） signals span a wide range of frequency, wireless signals coming from other communication systems may be aliased and appear as image interference. In quadrature intermed...Since Global Navigation Satellite System（GNSS） signals span a wide range of frequency, wireless signals coming from other communication systems may be aliased and appear as image interference. In quadrature intermediate frequency（IF） receivers, image aliasing due to in-phase and quadrature（I/Q） channel mismatches is always a big problem. I/Q mismatches occur because of gain and phase imbalances between quadrature mixers and capacitor mismatches in analog-to-digital converters（ADC）. As a result, the dynamic range and performance of a receiver are severely degraded. In this paper, several popular receiver architectures are summarized and the image aliasing problem is investigated in detail. Based on this analysis, a low-IF architecture is proposed for a single-chip solution and a novel and feasible anti-image algorithm is investigated. With this anti-image digital processing, the image reject ratio（IRR） can reach approximately above50 dB, which relaxes image rejection specific in front-end circuit designs and allows cheap and highly flexible analog front-end solutions. Simulation and experimental data show that the antiimage algorithm can work effectively, robustly, and steadily.展开更多
Talin protein was partially purified from human colorectal carcinoma tissues, which was subject to tryptic digestion. Immunoaffinity precipitation with specific antibodies that recognize diglycyl-lysine(Lys) remnants ...Talin protein was partially purified from human colorectal carcinoma tissues, which was subject to tryptic digestion. Immunoaffinity precipitation with specific antibodies that recognize diglycyl-lysine(Lys) remnants from tryptic digestion of ubiquitinated peptides was used to enrich ubiquitinated sites in talin. Mass spectrometry coupled with capillary reverse-phase high-performance liquid chromatography was used to analyze tlie enriched peptides. Specifically, four peptides containing diglycyl-Lys remnants from talin, namely, TAK(ub)VLVEDTK, QQQYK(ub) FLPSELRDEH, K(ub)STVLQQQYNR, and EGILK(ub)TAK can be determined using mass spectrometric data. This study provides an analytical method for further study in tlie relationship between ubiquitination modification of talin and its biological activity in colorectal cancer tissues with different pathological processes.展开更多
Human periostin was over-expressed in HEK293T cells, which was enriched by nickel ion affinity resin, and further purified by gel electrophoresis. Phosphopeptides contained in the tryptic digestion of the purified per...Human periostin was over-expressed in HEK293T cells, which was enriched by nickel ion affinity resin, and further purified by gel electrophoresis. Phosphopeptides contained in the tryptic digestion of the purified periostin were enriched by TiO2 affinity chromatography, and then analyzed by liquid chromatography-tandem mass spectrometry（LC-MS/MS）. LC-MS/MS analysis reveals three phosphorylation modification sites of periostin at IKVIEGpSLQPIIK（682-694）, pSLHEKLKQDK（498-507） and p[TT]VLYEC^＊C^＊PGYM^＊R（73-85）. The established method could be a great help to profiling the phosphorylation status of periostin under different physiological environments, such as inflammatory and tumor micro-environments.展开更多
In order to identify potential protein targets involved in colorectal cancer（CRC）, we used a liquid chro- matography coupled with mass spectrometry（LC-MS）/MS-based proteomics approach to characterize global protei...In order to identify potential protein targets involved in colorectal cancer（CRC）, we used a liquid chro- matography coupled with mass spectrometry（LC-MS）/MS-based proteomics approach to characterize global protein expression patterns in malignant tissues and their adjacent healthy tissues from Dukes C stage CRC patients. A total number of 34 differentially expressed proteins were detected and identified by LC-MS/MS and database searching, which are supposed to be relevant to progression of colorectal tumor. Among these proteins, nucleophosmin I（NPM1） was found to be remarkably up-regulated in colorectal carcinoma tissues, as compared with that in their normal counterparts. The results presented here could provide clues to elucidate the pathological significance of NPM1 in regulation of carcinogenesis of Dukes C stage colorectal tumors.展开更多
基金Supported by the National Natural Science Foundation of China（No. 81572082） and the Natural Science Foundation of Jilin Province, China（Nos.2011713, 20150414015GH, 20150204010YY）.
文摘The effect of the chelating agent 4,7-bis（chlorosulfophenyl）-1,10-phenanthroline-2,9-dicarboxylic acid （BCPDA） labeled hepatitis B surface antibodies（HBsAb） on time-resolved fluoroimmunoassays（TRFIA） was investigated. The labeling detection method was established. The biotin-streptavidin（BAS） system was introduced, and the multi-stage amplification effect of the BAS system was analyzed. It is found that the BCPDA-Eu^3＋-HBsAb-BAS fluorescent marker can emit strong fluorescence. Compared to BCPDA-Eu^3＋-HBsAb, BCPDA-Eu^3＋-HBsAb-BAS demonstrates an enhanced fluorescence intensity by over 10 times. This study provides a guidance for the next generation non-radio immunoassays in clinical diagnosis,
基金co-supported by Western Light Talent Culture Project of China(No.2013BS25)the National Natural Science Foundation of China(No.11203027)
文摘Since Global Navigation Satellite System（GNSS） signals span a wide range of frequency, wireless signals coming from other communication systems may be aliased and appear as image interference. In quadrature intermediate frequency（IF） receivers, image aliasing due to in-phase and quadrature（I/Q） channel mismatches is always a big problem. I/Q mismatches occur because of gain and phase imbalances between quadrature mixers and capacitor mismatches in analog-to-digital converters（ADC）. As a result, the dynamic range and performance of a receiver are severely degraded. In this paper, several popular receiver architectures are summarized and the image aliasing problem is investigated in detail. Based on this analysis, a low-IF architecture is proposed for a single-chip solution and a novel and feasible anti-image algorithm is investigated. With this anti-image digital processing, the image reject ratio（IRR） can reach approximately above50 dB, which relaxes image rejection specific in front-end circuit designs and allows cheap and highly flexible analog front-end solutions. Simulation and experimental data show that the antiimage algorithm can work effectively, robustly, and steadily.
基金the Science and Technology Department of Jilin Province,China(No.20150414015GH)the National Natural Science Foundation of China(Nos.81572082, 81472454).
文摘Talin protein was partially purified from human colorectal carcinoma tissues, which was subject to tryptic digestion. Immunoaffinity precipitation with specific antibodies that recognize diglycyl-lysine(Lys) remnants from tryptic digestion of ubiquitinated peptides was used to enrich ubiquitinated sites in talin. Mass spectrometry coupled with capillary reverse-phase high-performance liquid chromatography was used to analyze tlie enriched peptides. Specifically, four peptides containing diglycyl-Lys remnants from talin, namely, TAK(ub)VLVEDTK, QQQYK(ub) FLPSELRDEH, K(ub)STVLQQQYNR, and EGILK(ub)TAK can be determined using mass spectrometric data. This study provides an analytical method for further study in tlie relationship between ubiquitination modification of talin and its biological activity in colorectal cancer tissues with different pathological processes.
基金Supported by the National Natural Science Foundation of China（Nos.81472454, 31100930）, the Natural Science Foundation of Jilin Provincial Science and Technology Department, China（Nos.2011713, 20090461, 20110739, 20130413017GH）, the Natural Science Foundation of Heilongjiang Province, China（Nos.201000742, D201075, Gc09c317）, the Natural Science Foundation of Jilin Provincial Development and Reform Commission, China（No.2013c026-5） and the Natural Science Foundation of Changchun Science and Technology Bureau, China（No.2011125）.
文摘Human periostin was over-expressed in HEK293T cells, which was enriched by nickel ion affinity resin, and further purified by gel electrophoresis. Phosphopeptides contained in the tryptic digestion of the purified periostin were enriched by TiO2 affinity chromatography, and then analyzed by liquid chromatography-tandem mass spectrometry（LC-MS/MS）. LC-MS/MS analysis reveals three phosphorylation modification sites of periostin at IKVIEGpSLQPIIK（682-694）, pSLHEKLKQDK（498-507） and p[TT]VLYEC^＊C^＊PGYM^＊R（73-85）. The established method could be a great help to profiling the phosphorylation status of periostin under different physiological environments, such as inflammatory and tumor micro-environments.
基金Supported by the National Natural Science Foundation of China（Nos.81572082, 81472030, 20973074）, the Science Founda tion of Jilin Provincial Science＆Technology Department, China（No.2011713, 20130413017GH, 20150414015GH）, the Health and Family Planning Commission of Jilin Province, China（No.2015Z041） and the Scientific Support Project from Bethune Department of Jilin University, China（Nos.2012210, 2013207051）.
文摘In order to identify potential protein targets involved in colorectal cancer（CRC）, we used a liquid chro- matography coupled with mass spectrometry（LC-MS）/MS-based proteomics approach to characterize global protein expression patterns in malignant tissues and their adjacent healthy tissues from Dukes C stage CRC patients. A total number of 34 differentially expressed proteins were detected and identified by LC-MS/MS and database searching, which are supposed to be relevant to progression of colorectal tumor. Among these proteins, nucleophosmin I（NPM1） was found to be remarkably up-regulated in colorectal carcinoma tissues, as compared with that in their normal counterparts. The results presented here could provide clues to elucidate the pathological significance of NPM1 in regulation of carcinogenesis of Dukes C stage colorectal tumors.
