Anthropogenic nutrient discharge poses widespread threats to coastal ecosystems and has increased environmental gradients from coast to sea. Bacterioplankton play crucial roles in coastal biogeochemical cycling, and a...Anthropogenic nutrient discharge poses widespread threats to coastal ecosystems and has increased environmental gradients from coast to sea. Bacterioplankton play crucial roles in coastal biogeochemical cycling, and a variety of factors af fect bacterial community diversity and structure. We used 16 S r RNA gene pyrosequencing to investigate the spatial variation in bacterial community composition(BCC) across five sites on a coast-of fshore gradient in the East China Sea. Overall, bacterial alpha-diversity did not diff er across sites, except that richness and phylogenetic diversity were lower in the of fshore sites, and the highest alpha-diversity was found in the most landward site, with Chl-abeing the main factor. BCCs generally clustered into coastal and of fshore groups. Chl-a explained 12.3% of the variation in BCCs, more than that explained by either the physicochemical(5.7%) or spatial(8.5%) variables. Nutrients(particularly nitrate and phosphate), along with phytoplankton abundance, were more important than other physicochemical factors, co-explaining 20.0% of the variation in BCCs. Additionally, a series of discriminant families(primarily affiliated with Gammaproteobacteria and Alphaproteobacteria), whose relative abundances correlated with Chl-a, DIN, and phosphate concentrations, were identified, implying their potential to indicate phytoplankton blooms and nutrient enrichment in this marine ecosystem. This study provides insight into bacterioplankton response patterns along a coast-of fshore gradient, with phytoplankton abundance increasing in the of fshore sites. Time-series sampling across multiple transects should be performed to determine the seasonal and spatial patterns in bacterial diversity and community structure along this gradient.展开更多
目的分析200例无生机的未足月胎膜早破(Previable preterm premature rupture of the membranes,PPROM)孕妇的生殖道感染病原菌的分布及预后。方法选自2003年1月~2017年1月在我院就诊的200例14~23周无生机儿PPROM者,知情选择后强烈要求...目的分析200例无生机的未足月胎膜早破(Previable preterm premature rupture of the membranes,PPROM)孕妇的生殖道感染病原菌的分布及预后。方法选自2003年1月~2017年1月在我院就诊的200例14~23周无生机儿PPROM者,知情选择后强烈要求保胎者,明确诊断入组后予阴道宫颈分泌物解脲及人型支原体,衣原体,淋球菌,加德纳菌,念珠菌,阴道毛滴虫,链球菌等培养。结果200例中95例支原体阳性(其中89例解脲支原体阳性,39例人型支原体阳性,33例两者都阳性),11例在衣原体阳性,7例淋球菌阳性,46例加德纳菌阳性,45例假丝酵母菌阳性,31例B-链球菌阳性,滴虫6例,17例病原菌监测阴性。其中三种以上病原菌感染23例,两种感染56例,一种感染104例,病原菌总感染率高达91.5%,而且病原菌感染种数越多,不良妊娠几率越高且出现越早[1]。积极抗炎对症治疗后其中40例成功保胎至>23周,随访新生儿至一周岁内均无明显不良预后。结论对于未足月胎膜早破,需常规进行阴道病原菌检查,及时明确诊断,积极进行抗感染治疗或预防性抗生素治疗对稳定患者生殖道感染及扩散,改善妊娠预后具有重要意义。展开更多
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA092001)the National Natural Science Foundation of China(No.41406118)+4 种基金the Zhejiang Provincial Natural Science Foundation of China(No.LQ14D060003)the Social Development Project of Ningbo(No.2014C50009)the Natural Science Foundation of Ningbo University(No.XKZSC1421)the Research Fund from the Collaborative Innovation Center for Zhejiang Marine HighEfficiency and Healthy Aquaculture,Ningbo,Chinathe KC Wong Magna Fund in Ningbo University
文摘Anthropogenic nutrient discharge poses widespread threats to coastal ecosystems and has increased environmental gradients from coast to sea. Bacterioplankton play crucial roles in coastal biogeochemical cycling, and a variety of factors af fect bacterial community diversity and structure. We used 16 S r RNA gene pyrosequencing to investigate the spatial variation in bacterial community composition(BCC) across five sites on a coast-of fshore gradient in the East China Sea. Overall, bacterial alpha-diversity did not diff er across sites, except that richness and phylogenetic diversity were lower in the of fshore sites, and the highest alpha-diversity was found in the most landward site, with Chl-abeing the main factor. BCCs generally clustered into coastal and of fshore groups. Chl-a explained 12.3% of the variation in BCCs, more than that explained by either the physicochemical(5.7%) or spatial(8.5%) variables. Nutrients(particularly nitrate and phosphate), along with phytoplankton abundance, were more important than other physicochemical factors, co-explaining 20.0% of the variation in BCCs. Additionally, a series of discriminant families(primarily affiliated with Gammaproteobacteria and Alphaproteobacteria), whose relative abundances correlated with Chl-a, DIN, and phosphate concentrations, were identified, implying their potential to indicate phytoplankton blooms and nutrient enrichment in this marine ecosystem. This study provides insight into bacterioplankton response patterns along a coast-of fshore gradient, with phytoplankton abundance increasing in the of fshore sites. Time-series sampling across multiple transects should be performed to determine the seasonal and spatial patterns in bacterial diversity and community structure along this gradient.
文摘目的分析200例无生机的未足月胎膜早破(Previable preterm premature rupture of the membranes,PPROM)孕妇的生殖道感染病原菌的分布及预后。方法选自2003年1月~2017年1月在我院就诊的200例14~23周无生机儿PPROM者,知情选择后强烈要求保胎者,明确诊断入组后予阴道宫颈分泌物解脲及人型支原体,衣原体,淋球菌,加德纳菌,念珠菌,阴道毛滴虫,链球菌等培养。结果200例中95例支原体阳性(其中89例解脲支原体阳性,39例人型支原体阳性,33例两者都阳性),11例在衣原体阳性,7例淋球菌阳性,46例加德纳菌阳性,45例假丝酵母菌阳性,31例B-链球菌阳性,滴虫6例,17例病原菌监测阴性。其中三种以上病原菌感染23例,两种感染56例,一种感染104例,病原菌总感染率高达91.5%,而且病原菌感染种数越多,不良妊娠几率越高且出现越早[1]。积极抗炎对症治疗后其中40例成功保胎至>23周,随访新生儿至一周岁内均无明显不良预后。结论对于未足月胎膜早破,需常规进行阴道病原菌检查,及时明确诊断,积极进行抗感染治疗或预防性抗生素治疗对稳定患者生殖道感染及扩散,改善妊娠预后具有重要意义。
