Radiation-Induced Chromosome Instability in WTK1 and TK6 Human Lymphoblastoid Cells

The effects of ionizing radiation on single nucleotide polymorphism (SNP) copy number variations between TK6 and WTK1 cell lines are described herein. Specifically, the integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) was analyzed in the presence and absence of ionizing radiation. WTK1 cells contain a p53 mutation, whereas the TK6 cell line has the native p53 tumor-suppressor gene. Each cell line was isolated post-irradiation for SNP analysis, which showed significant, genome-wide impacts on both cell lines;for the mutant WTK1 sample, there were a total of 48 gene deletions and no gene amplifications, whereas for the wild-type TK6 sample, there were 217 gene deletions and 9 gene amplifications. It appears that both cell lines are affected in the areas of cell-cycle control, but that other affected areas differ significantly between the two.

Perceptions and Attitudes Towards Shoulder Padding and Shoulder Injury in Rugby Union

Purpose To develop an understanding of the role of shoulder padding in rugby union by investigating player perceptions and attitudes towards shoulder padding and extending research into shoulder injuries in rugby.Methods An online survey was distributed to past and current rugby players over 13 years old in 2018.Questions related to the participants’demographic,attitudes to shoulder padding and shoulder injury history.Results Six hundred and sixteen rugby players responded to the survey;66.1%of respondents had worn shoulder padding at some point.The age group 24-29 years old(ΔR^(2)=0.03,B=−0.53,P=0.015)had an inverse association with padding effectiveness while playing experience groups 1-2 years(ΔR^(2)=0.03,B=0.8,P=0.032),3-5 years(ΔR^(2)=0.03,B=0.70,P=0.002)and 6-9 years(ΔR^(2)=0.03,B=0.41,P=0)had a positive association.There are 37.1%of respondents consider-ing shoulder padding to be effective at preventing cuts and abrasions with 21.9%finding it very effective;50.3%considered it to be effective or very effective(9.7%)at preventing contusion;45.5%wore padding for injury prevention,while 19.2%wore padding to protect from reoccurring injury.Sprain/ligament damage(57.5%)and bruising(55.5%)were the most com-monly reported injuries.Conclusions The primary reason for wearing shoulder padding was as a means of injury prevention.Research should focus on quantifying the injury preventive capabilities of shoulder padding.Bruising,cuts and abrasion injuries to the shoulder are prevalent presenting new findings that these injuries are underreported.