For a safe and sustainable environment,effective microbes as biocontrol agents are in high demand.We have isolated a new Bacillus velezensis strain DTU001,investigated its antifungal spectrum,sequenced its genome,and ...For a safe and sustainable environment,effective microbes as biocontrol agents are in high demand.We have isolated a new Bacillus velezensis strain DTU001,investigated its antifungal spectrum,sequenced its genome,and uncovered the production of lipopeptides in HPLC-HRMS analysis.To test the antifungal efficacy,extracts of B.velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi.We demonstrate that inhibitory potential of B.velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide,either iturin or fengycin.The isolate showed analogous biofilm formation to other closely related Bacilli.To further support the biocontrol properties of the isolate,coculture with Candida albicans demonstrated that B.velezensis DTU001 exhibited excellent antiproliferation effect against C.albicans.In summary,the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.展开更多
基金This work was supported by the Danish National Research Foundation(DNRF137)for the Center for Microbial Secondary Metabolites(CeMiSt)SD was supported by a Novozymes and Henning Holck Larsen fellowship during her stay at DTU.TW was supported by a grant from Novo Nordisk Foundation(NNF10CC1016517).
文摘For a safe and sustainable environment,effective microbes as biocontrol agents are in high demand.We have isolated a new Bacillus velezensis strain DTU001,investigated its antifungal spectrum,sequenced its genome,and uncovered the production of lipopeptides in HPLC-HRMS analysis.To test the antifungal efficacy,extracts of B.velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi.We demonstrate that inhibitory potential of B.velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide,either iturin or fengycin.The isolate showed analogous biofilm formation to other closely related Bacilli.To further support the biocontrol properties of the isolate,coculture with Candida albicans demonstrated that B.velezensis DTU001 exhibited excellent antiproliferation effect against C.albicans.In summary,the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.
