Dear Editor,Compared with traditional technologies affecting gene expression,changing DNA sequences of target genes is one of the most outstanding characters of CRISPR(Clustered Regularly Interspaced Short Palindromic...Dear Editor,Compared with traditional technologies affecting gene expression,changing DNA sequences of target genes is one of the most outstanding characters of CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats).Single-guide RNAs(sgRNAs)guiding endonuclease Cas to target sites is a crucial step of CRISPR-Cas system for changing DNA sequences.An ideal sgRNA should only bind to the target gene.However,similar sequences of non-target sites can also be recognized leading to off-target effects[1].展开更多
基金This work was supported by National Natural Science Foundation of China(Grant No.32100501)Shenzhen Science and Technology Program(Grant No.RCBS20210609103819020)J.Z.was funded by the Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding(2021C02070-1).
文摘Dear Editor,Compared with traditional technologies affecting gene expression,changing DNA sequences of target genes is one of the most outstanding characters of CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats).Single-guide RNAs(sgRNAs)guiding endonuclease Cas to target sites is a crucial step of CRISPR-Cas system for changing DNA sequences.An ideal sgRNA should only bind to the target gene.However,similar sequences of non-target sites can also be recognized leading to off-target effects[1].
