Objective:To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-αexpression, spiral artery remodeling, and placental apoptosis.Methods: The study used pregnant mice divided to five groups of treatment...Objective:To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-αexpression, spiral artery remodeling, and placental apoptosis.Methods: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment;Group 2, 1/12 LD50 of lead and Aquadest;Group 3, 1/16 LD50 of lead and Aquadest, Group 4, 1/12 LD50 of lead and 1×107 HSC;and group 5, 1/16 LD50 of lead and 1×107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF-α expression and the apoptosis index. The data from all five groups were compared using the ANOVA test.Results:TNF-α expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P<0.01). There was also a significant difference of spiral artery remodeling between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment (P<0.05). In apoptosis index evaluation there was a significant difference between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment, but not in the groups of 1/16 LD50 of lead followed HSC (P>0.05).Conclusions:In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF-α expression, spiral artery remodeling, and placental apoptosis index but in the group of 1/16 LD50 of lead and HSC treatment, apoptosis index has no differences with those of control group.展开更多
The patient with dysmenorrhea was treated by needling at Tàichōng（太冲 LR 3）, 4 times of treatment was given and the nausea, migraine and pain scale of the patient were alleviated and finally disappeared.Until...The patient with dysmenorrhea was treated by needling at Tàichōng（太冲 LR 3）, 4 times of treatment was given and the nausea, migraine and pain scale of the patient were alleviated and finally disappeared.Until the next two menstrual period, patient did not have similar complaints. The author recommended for other acupuncturists to utilize LR 3 as complementary therapy for relieving pain at primary dysmenorrhea.展开更多
文摘Objective:To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-αexpression, spiral artery remodeling, and placental apoptosis.Methods: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment;Group 2, 1/12 LD50 of lead and Aquadest;Group 3, 1/16 LD50 of lead and Aquadest, Group 4, 1/12 LD50 of lead and 1×107 HSC;and group 5, 1/16 LD50 of lead and 1×107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF-α expression and the apoptosis index. The data from all five groups were compared using the ANOVA test.Results:TNF-α expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P<0.01). There was also a significant difference of spiral artery remodeling between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment (P<0.05). In apoptosis index evaluation there was a significant difference between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment, but not in the groups of 1/16 LD50 of lead followed HSC (P>0.05).Conclusions:In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF-α expression, spiral artery remodeling, and placental apoptosis index but in the group of 1/16 LD50 of lead and HSC treatment, apoptosis index has no differences with those of control group.
文摘The patient with dysmenorrhea was treated by needling at Tàichōng（太冲 LR 3）, 4 times of treatment was given and the nausea, migraine and pain scale of the patient were alleviated and finally disappeared.Until the next two menstrual period, patient did not have similar complaints. The author recommended for other acupuncturists to utilize LR 3 as complementary therapy for relieving pain at primary dysmenorrhea.
