Adriamycin(doxorubicin),a common cancer chemotherapeutic drug,can be used to induce a model of chronic progressive glomerular disease in rodents.In our studies,we evahuated renal changes in a rat model after Adriamydi...Adriamycin(doxorubicin),a common cancer chemotherapeutic drug,can be used to induce a model of chronic progressive glomerular disease in rodents.In our studies,we evahuated renal changes in a rat model after Adriamydin injection using two photon microscopy(TPM),optical coherence tomography(OCT)and Doppler OCT(DOCT).Taking advantage of deep penetra-tion and fast scanning speed for three dimensional(3D)label-free imaging,OCT/DOCT system was able to reveal glomerular and tubular pathology noninvasively and in real time.By imaging renal pathology following the infusion of fAuorophore-labeled dextrans of different molecular weights,TPM can provide direct views of glomerular and tubular flow dynamics with the onset and progression of renal disease.Specifically,glomerular permeability and filtration,proximal and distal tubular flow dynamics can be revealed.6-8 weeks after injection of Adriamycin,TPM and OCT/DOCT imaging revealed glomerular sclerosis,compromised flow across the glomerular wall,tubular atrophy,tubular dilation,and variable intra-tubular flow dynamics.Our results indicate that TPM and OCT/DOCT provide real-time imaging of renal pathology in vivo that has not been previously available using conventional microscopic procedures.展开更多
In the past two decades, two-photon microscopy （TPM） transforms biomedicalresearch, allowing non- destructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we re...In the past two decades, two-photon microscopy （TPM） transforms biomedicalresearch, allowing non- destructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technologyincluding novel laser sources, new image acquisition paradiams, and microendoscopicimaging systems. Then, we survey the capabilities of TPM imagingof biological relevant molecules such as nicotinamideadenine dinucleotide （NADH）, flavin adenine dinucleotide （FAD）,and reactive oxygen species （ROS）. Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.展开更多
基金the National Institutes of Health(NIH)Grant Nos.R21AG042700 and R21DK088066。
文摘Adriamycin(doxorubicin),a common cancer chemotherapeutic drug,can be used to induce a model of chronic progressive glomerular disease in rodents.In our studies,we evahuated renal changes in a rat model after Adriamydin injection using two photon microscopy(TPM),optical coherence tomography(OCT)and Doppler OCT(DOCT).Taking advantage of deep penetra-tion and fast scanning speed for three dimensional(3D)label-free imaging,OCT/DOCT system was able to reveal glomerular and tubular pathology noninvasively and in real time.By imaging renal pathology following the infusion of fAuorophore-labeled dextrans of different molecular weights,TPM can provide direct views of glomerular and tubular flow dynamics with the onset and progression of renal disease.Specifically,glomerular permeability and filtration,proximal and distal tubular flow dynamics can be revealed.6-8 weeks after injection of Adriamycin,TPM and OCT/DOCT imaging revealed glomerular sclerosis,compromised flow across the glomerular wall,tubular atrophy,tubular dilation,and variable intra-tubular flow dynamics.Our results indicate that TPM and OCT/DOCT provide real-time imaging of renal pathology in vivo that has not been previously available using conventional microscopic procedures.
基金H. Guo andY. Chen are supported by National Institutes of Health(NIH)under Grant Nos.R21AG042700,R21DK088066,and R21EB012215L. Qin and S. Cho are supported by NIH under Grant No.R01HL082511+1 种基金H. Aleyasin and R. R. Ratan are supported by NIH under Grant Nos.P01AG14930-10,NS04059,and NS39170 W. Gong, J.Chen, and S. Xie are supported by the National Natural Science Foundation of China under Grant No.60678054
文摘In the past two decades, two-photon microscopy （TPM） transforms biomedicalresearch, allowing non- destructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technologyincluding novel laser sources, new image acquisition paradiams, and microendoscopicimaging systems. Then, we survey the capabilities of TPM imagingof biological relevant molecules such as nicotinamideadenine dinucleotide （NADH）, flavin adenine dinucleotide （FAD）,and reactive oxygen species （ROS）. Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.
