Biochar has been considered an effective approach as soil amendment for decreasing incidences of disease and regulating microbial populations in continuous-cropping soil.Although researches have extensively focused on...Biochar has been considered an effective approach as soil amendment for decreasing incidences of disease and regulating microbial populations in continuous-cropping soil.Although researches have extensively focused on changes of soil microbes and unbalance of nutrition in continuous-cropping soil,the relationship between soil properties and pathogens by biochar application remains poorly understood.In this study,we applied ITS ribosomal RNA gene profiling to analyze tobacco root microbiota of biochar and non-biochar treatment in a 3-year continuous-cropping tobacco field,comparing firstly planting tobacco as control.We found that biochar application decreased the relative abundance of the soil fungal pathogens(Ceratobasidium and Monosporascus),which are the prime pathogens of tobacco root rot in continuous-cropping soil.Using RDA,co-occurrence and PLS-PM approaches,we provided evidence that there was a negative correlation between fungal genera(especially for Ceratobasidium and Monosporascus)and soil polyphenol oxidase(PPO)activity(R^(2)_(incidence rate)=−0.930,R^(2)_(disease index)=−0.905,both p<0.001).The PPO was up-regulated by different biochar treatment intensities.Together,we demonstrated that biochar in continuouscropping soil regulated the soil PPO activity to suppress pathogens,and further decrease incidence of root rot.Notably,biochar application forward continuous cropping was more effective for the continuous-cropping soil improvement than the other treatments.The data should help in appropriate timing of biochar application for alleviating continuous-cropping obstacle.展开更多
基金Guiding Fund of the Central Government for Local Science and Technology Development,China(2023JH6/100100056)Earmarked Fund for Modern Agroindustry Technology Research System,China(No.CARS-01-51)+2 种基金S&D Program from Bijie Tobacco Company of Guizhou Province(2021520500240048,2018520500240066)Young Elite Scientists Sponsorship Program by CAST-YESS“Research on the Mechanism of Biochar Effect of Improving Soil Properties and Microecological in Tobacco Planting Soil”,National Natural Science Foundation of China(31901449)Science and Technology Program Project of Liaoning Province(2021-MS-232).
文摘Biochar has been considered an effective approach as soil amendment for decreasing incidences of disease and regulating microbial populations in continuous-cropping soil.Although researches have extensively focused on changes of soil microbes and unbalance of nutrition in continuous-cropping soil,the relationship between soil properties and pathogens by biochar application remains poorly understood.In this study,we applied ITS ribosomal RNA gene profiling to analyze tobacco root microbiota of biochar and non-biochar treatment in a 3-year continuous-cropping tobacco field,comparing firstly planting tobacco as control.We found that biochar application decreased the relative abundance of the soil fungal pathogens(Ceratobasidium and Monosporascus),which are the prime pathogens of tobacco root rot in continuous-cropping soil.Using RDA,co-occurrence and PLS-PM approaches,we provided evidence that there was a negative correlation between fungal genera(especially for Ceratobasidium and Monosporascus)and soil polyphenol oxidase(PPO)activity(R^(2)_(incidence rate)=−0.930,R^(2)_(disease index)=−0.905,both p<0.001).The PPO was up-regulated by different biochar treatment intensities.Together,we demonstrated that biochar in continuouscropping soil regulated the soil PPO activity to suppress pathogens,and further decrease incidence of root rot.Notably,biochar application forward continuous cropping was more effective for the continuous-cropping soil improvement than the other treatments.The data should help in appropriate timing of biochar application for alleviating continuous-cropping obstacle.
