Background: The public health nurse should make efforts to keep his/her own mental and physical health good, which is the professional responsibility. However, a variety of challenging issues have been pointed out wit...Background: The public health nurse should make efforts to keep his/her own mental and physical health good, which is the professional responsibility. However, a variety of challenging issues have been pointed out with regard to well-being of healthcare professionals. Public health nurses are likely to face some difficulties in their habits of leading healthy lifestyles. The purpose of this study was to identify how Japanese public health nurses actually live their healthy lives. Methods: This study covered 1725 public health nurses in the Chugoku/Shikoku Region. We conducted an anonymous self-completed questionnaire survey. As basic attributes, the following 6 categories were set. The health practice indices used in this research consisted of the 7 practices of Breslow’s Health Practice Index (B-HPI), the Morimoto’s 8 practices, and 6 practices related to lifestyle habits that have been developed independently by the authors of this research (Health Lifestyle Index (HLI)), that is, 16 practices in total. The study plan was approved by the Ethics Committee of Okayama University. Results: We analyzed the 960 (55.7%) valid responses. This survey has demonstrated that the healthy life-style practices of public health nurses are generally good. However, there were some practices such as eating between meals, having little physical activity, and not having a good rest/sleep, that need to be improved. None of the assessment tools correlated with the length of experience. Conclusions: This survey has clarified that the healthy lifestyle practices of public health nurses did not correlated with their length of experience, indicating that the healthy lifestyle practices are not intensified by the accumulated career experience as public health nurses.展开更多
α-Synuclein is the major component of the filamentous Lewy bodies and Lewy neurites that define neuropathological features and dementia with Lewy bodies. To investigate the role of dopamine (DA) in α-synuclein fibri...α-Synuclein is the major component of the filamentous Lewy bodies and Lewy neurites that define neuropathological features and dementia with Lewy bodies. To investigate the role of dopamine (DA) in α-synuclein fibrillation, the structural propensities to form oligomers at the initial stage fibrillation were studied using size exclusion chromatography and various biophysical techniques. Interactions with DA were observed for wild-type α-synuclein and its mutants, A30P, E46K and A53T, using electrospray ionization mass spectrometry (ESI-MS). The results of ESI-MS indicate that an intact α-synuclein, which was not oxidized, had an ability to bind with three molecules of DA at the initial stage. Furthermore, upon binding to DA, α-synuclein oligomerizes to higher molecular weight species. These oligomers are structurally different from amyloid fibrils, as confirmed by thioflavin T and CD analysis.展开更多
文摘Background: The public health nurse should make efforts to keep his/her own mental and physical health good, which is the professional responsibility. However, a variety of challenging issues have been pointed out with regard to well-being of healthcare professionals. Public health nurses are likely to face some difficulties in their habits of leading healthy lifestyles. The purpose of this study was to identify how Japanese public health nurses actually live their healthy lives. Methods: This study covered 1725 public health nurses in the Chugoku/Shikoku Region. We conducted an anonymous self-completed questionnaire survey. As basic attributes, the following 6 categories were set. The health practice indices used in this research consisted of the 7 practices of Breslow’s Health Practice Index (B-HPI), the Morimoto’s 8 practices, and 6 practices related to lifestyle habits that have been developed independently by the authors of this research (Health Lifestyle Index (HLI)), that is, 16 practices in total. The study plan was approved by the Ethics Committee of Okayama University. Results: We analyzed the 960 (55.7%) valid responses. This survey has demonstrated that the healthy life-style practices of public health nurses are generally good. However, there were some practices such as eating between meals, having little physical activity, and not having a good rest/sleep, that need to be improved. None of the assessment tools correlated with the length of experience. Conclusions: This survey has clarified that the healthy lifestyle practices of public health nurses did not correlated with their length of experience, indicating that the healthy lifestyle practices are not intensified by the accumulated career experience as public health nurses.
文摘α-Synuclein is the major component of the filamentous Lewy bodies and Lewy neurites that define neuropathological features and dementia with Lewy bodies. To investigate the role of dopamine (DA) in α-synuclein fibrillation, the structural propensities to form oligomers at the initial stage fibrillation were studied using size exclusion chromatography and various biophysical techniques. Interactions with DA were observed for wild-type α-synuclein and its mutants, A30P, E46K and A53T, using electrospray ionization mass spectrometry (ESI-MS). The results of ESI-MS indicate that an intact α-synuclein, which was not oxidized, had an ability to bind with three molecules of DA at the initial stage. Furthermore, upon binding to DA, α-synuclein oligomerizes to higher molecular weight species. These oligomers are structurally different from amyloid fibrils, as confirmed by thioflavin T and CD analysis.
