2D Numerical Simulation of Liver Cell Proliferation with Angiogenesis for Hepatic Lobule Formation

The liver has the ability to reform and regenerate in our body. However, the mechanisms of reformation or regeneration of the liver have not been elucidated. In this study, we propose an analysis model using a Particle Model to elucidate the mechanism of liver formation. The object of analysis is a hepatic lobule, which is the basic component of the liver. First, a 2-dimensional cell proliferation around one blood vessel was modeled. Second, angiogenesis was added and considered. And finally, the model was applied to the hepatic lobule and the 2D formation of the hepatic lobule was revealed. We used experimentally derived parameters such as diffusivity, oxygen concentration, and oxygen consumption of a cell. The model will be expected to facilitate in developing tissue-engineered liver using regenerative medicine technology.

Heparin/Growth Factors‑Immobilized Aligned Electrospun Nanofibers Promote Nerve Regeneration in Polycaprolactone/Gelatin‑Based Nerve Guidance Conduits

Injuries to the nervous system account for the widespread morbidity,mortality,and discomfort worldwide.Artificial nerve guidance conduits(NGCs)offer a promising platform for nerve reconstruction,however,they require extracellular matrix(ECM)-like features to better mimic the in vivo microenvironment.Consequently,this research was aimed to fabricate heparin/growth factors(GFs)-immobilized artificial NGCs.Heparin was covalently immobilized onto aligned electrospun polycapro-lactone/gelatin(PCL/Gel)nanofibers.Thereafter,basic fibroblast growth factor(bFGF)and nerve growth factor(NGF)were preferentially immobilized on heparinized nanofibers;the immobilization efficiency of GFs was found to be 50%with respect to(w.r.t.)their initial loaded amounts.The in vivo implantation of NGCs in a sciatic nerve defect model revealed the successful retention(~10%w.r.t the initial loaded amount)and bioactivity of NGF for up to 5 days.The permeability of bovine serum albumin(BSA)from nanofibrous membranes was further assessed and found to be comparable with the commercialized cel-lulose acetate membranes.The bioactivity of NGCs was assessed in a sciatic nerve defect model in rats for short-term(1 week)and long-term(1-month).The NGCs displayed good structural stability and biocompatibility in vivo.The in vivo evaluation revealed the accumulation of host cells into the transplanted NGCs.Taken together;these heparin/GFs-immobilized artificial NGCs may have broad implications for nerve regeneration and related tissue engineering disciplines.

Correction to:Heparin/Growth Factors‑Immobilized Aligned Electrospun Nanofibers Promote Nerve Regeneration in Polycaprolactone/Gelatin‑Based Nerve Guidance Conduits

Correction to:Advanced Fiber Materials https://doi.org/10.1007/s42765-022-00244-6 In this article the affiliation details for Author Muhammad Shafiq were incorrectly given.They should have been assigned as shown below.The original article has been corrected.