The Quantougou(QTG) Fauna in central Lanzhou Basin is an important late Mid-Miocene fauna on the northeastern Tibetan Plateau margin,but its numerical age remains a matter of debate.Here,we present a new magnetostrati...The Quantougou(QTG) Fauna in central Lanzhou Basin is an important late Mid-Miocene fauna on the northeastern Tibetan Plateau margin,but its numerical age remains a matter of debate.Here,we present a new magnetostratigraphic record for a fluvio-lacustrine section to further constrain the age of the QTG Fauna.Results suggest that the studied section spans from polarity chrons C5Cn.2n to C5 n.2n or C5 An.1n,with ages of ca.16.5 Ma to 10 Ma or 16.5 Ma to 12 Ma.The QTG Fauna is located at the top of polarity chron C5r.3 r or C5 Ar.2 r,which corresponds to an age of 11.7 Ma or 12.8 Ma for the fauna.Accordingly,the associated Myocricetodontinae(a subfamily of Gerbillidae,Rodentia) is suggested to have appeared in the Lanzhou Basin at 11.7 Ma or 12.8 Ma,which is the oldest Myocricetodontinae in East Asia but is still much younger than the ~20 Ma appearance of this subfamily in West and South Asia.Our age data support the interpretation that East Asian Myocricetodontinae originated from South Asia.The QTG fauna further suggest a dry and open grassland environment,which is consistent with global cooling after the Mid-Miocene Climatic Optimum.展开更多
Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs wer...Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs were obtained by computational prediction and screening, among which 72 were mapped to the porcine genome. Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species. Remarkably, one of the candidates, sTF35495, was found to be precursor of a putative porcine microRNA. By RACE PCR, we determined that the full length of sTF35495 was 3 kb. The protein-coding potential of this RNA was tested in silico with no significant finding. Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR. The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa.展开更多
基金This study was supported financially by the National Natural Science Foundation of China(Nos.41704071,41290253)the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0707)+2 种基金Chinese Academy of Sciences“Light of West China”Program(XAB2018B04)Australian Research Council grant DP190100874We thank David Heslop for checking our reversal test result.
文摘The Quantougou(QTG) Fauna in central Lanzhou Basin is an important late Mid-Miocene fauna on the northeastern Tibetan Plateau margin,but its numerical age remains a matter of debate.Here,we present a new magnetostratigraphic record for a fluvio-lacustrine section to further constrain the age of the QTG Fauna.Results suggest that the studied section spans from polarity chrons C5Cn.2n to C5 n.2n or C5 An.1n,with ages of ca.16.5 Ma to 10 Ma or 16.5 Ma to 12 Ma.The QTG Fauna is located at the top of polarity chron C5r.3 r or C5 Ar.2 r,which corresponds to an age of 11.7 Ma or 12.8 Ma for the fauna.Accordingly,the associated Myocricetodontinae(a subfamily of Gerbillidae,Rodentia) is suggested to have appeared in the Lanzhou Basin at 11.7 Ma or 12.8 Ma,which is the oldest Myocricetodontinae in East Asia but is still much younger than the ~20 Ma appearance of this subfamily in West and South Asia.Our age data support the interpretation that East Asian Myocricetodontinae originated from South Asia.The QTG fauna further suggest a dry and open grassland environment,which is consistent with global cooling after the Mid-Miocene Climatic Optimum.
基金supported by the National Natural Science Foundation of China (No.30830080 and 30800779)the Natural Science Foundation of Beijing (No.5072035)the Ministry of Science and Technology of China (No.2006AA10Z135,2008AA10Z143,2006CB102105 and 2009CB941604)
文摘Messenger RNA-like non-coding RNAs （mlncRNAs） are a newly identified group of non-coding RNAs （ncRNAs） that may be involved in a number of critical cellular events. In this study, 93 candidate porcine mlncRNAs were obtained by computational prediction and screening, among which 72 were mapped to the porcine genome. Further analysis of 8 representative candidates revealed that these mlncRNA candidates are not highly conserved among species. Remarkably, one of the candidates, sTF35495, was found to be precursor of a putative porcine microRNA. By RACE PCR, we determined that the full length of sTF35495 was 3 kb. The protein-coding potential of this RNA was tested in silico with no significant finding. Semi-quantitative RT-PCR analysis of the subgroup of 8 candidates revealed two distinct expression profiles and two molecules were further validated by real-time PCR. The predicted pre-microRNA sequence in this study provides a potentially interesting insight into the in vivo function of porcine mlncRNAs and our findings suggest that they play key biological roles in Sus scrofa.
