Research on the Application of Intelligent Bionic Robot Horse in Juvenile Equestrian Teaching Case Study: Beijing Chaoyang Park Youth Equestrian Center

Research purposes:in this study,the intelligent bionic robotic horse is introduced into the equestrian teaching for teenagers,compared with the traditional teaching mode of using real horses.This research aims to explore the effectiveness of using intelligent bionic robotic horse in equestrian teaching for teenagers,as well as to promote the further development of equestrian teaching for teenagers in China,and to promote the introduction of new technology into the equestrian teaching area in the age of internet.Research methods:the methods used were literature method;mathematical statistics;interviewing the equestrian coaches who participated in the experiment;experimental method.The intelligent bionic robotic horse used in this research is the GETTAEN intelligent bionic robotic horse produced by Joy Game Technology Co.,Ltd.The bionic robotic horse is equipped with Internet technology,and the course is supervised and produced by senior coaches of China Equestrian Team.It also includes multiple operation modes.In this study,40 amateur students in Beijing Chaoyang Park Youth Equestrian Center were selected as the experimental subjects.Students will spend 40 h on studying how to ride a horse.Twenty(20)students in the experimental group,they are accommodated with 20 h of bionic robotic horse courses and 20 h of real horse course;20 students in the control group were taught in the traditional teaching mode with 40 h of real horse courses.Results:(1)in horseback physical fitness test,the average value of the control group was 101.9 s;325.6 s in the experimental group.Independent sample T test p<0.05 has significant difference,the horseback physical performance in experimental group is better than the control group.(2)in horseback physical balance test,the average value of the control group was 3.75,and the average value of the experimental group was 7.1.Independent sample T test p<0.05 has significant difference,the horseback physical balance test results in experimental group have significant difference,and the experimental group is better than the control group.(3)The interview method was used to interview the equestrian coaches who participated in the experiment,coaches think that the bionic robotic horse can speed up the learning progress and has a strong technical consolidation,especially for teaching amateurs;but for the time being,it cannot meet the training and improvement target of the actual horse control ability and the ability to grasp the route,and such experience is not real and good enough for senior students.Conclusion:using real horse and intelligent bionic robotic horse combined,one can improve the teaching effectiveness and promote students’adaptation to horseback and technical mastery.But for the time being,it is only suitable for students with weak foundation or zero foundation.The capability of intelligent bionic robotic horse needs to be strengthened,and technological innovation is needed to adapt to all kinds of students.

Coactivator p100 protein enhances histone acetyltransferase activity of CBP

Human p100 protein consists of four repeated domains of staphylococcal nuclease (SN)-like domain, as well as a tudor (TD) domain thereafter. We have previously shown that the SN-like domain of p100 interacted with STAT6 and the large subunit of RNA pol II , resulting in the enhancement of STAT6-mediated gene transcriptional activation. Here, we show that SN-like domain also interacted with CREB binding protein (CBP) and directly enhanced the acetyl transferase activity of CBP on his tone. On the other hand, overexpression of CBP alone had no ability to significantly increase STAT6 dependent transcriptional activation, however, together with p100 protein, sufficiently enhanced the activation of transcription which was in line with the previous result that p100 protein bridged STAT6 with CBP.

Respective IL-17A production by γδ T and Th17 cells and its implication in host defense against chlamydial lung infection

The role of IL-17A is important in protection against lung infection with Chlamydiae,an obligate intracellular bacterial pathogen.In this study,we explored the producers of IL-17A in chlamydial lung infection and specifically tested the role of major IL-17A producers in protective immunity.We found thatγδT cells and Th17 cells are the major producers of IL-17A at the early and later stages of chlamydial infection,respectively.Depletion ofγδT cells in vivo at the early postinfection(p.i.)stage,when mostγδT cells produce IL-17A,failed to alter Th1 responses and bacterial clearance.In contrast,the blockade of IL-17A at the time when IL-17A was mainly produced by Th17(day 7 p.i.)markedly reduced the Th1 response and increased chlamydial growth.The data suggest that theγδT cell is the highest producer of IL-17A in the very early stages of infection,but the protection conferred by IL-17A is mainly mediated by Th17 cells.In addition,we found that depletion ofγδT cells reduced IL-1αproduction by dendritic cells,which was associated with a reduced Th17 response.This finding is helpful to understand the variable role of IL-17A in different infections and to develop preventive and therapeutic approaches against infectious diseases by targeting IL-17A.

DNA Extraction Protocol for Biological Ingredient Analysis of Liuwei Dihuang Wan

Traditional Chinese medicine （TCM） clinical practice. So far, the methods commonly preparations are widely used for healthcare and used for quality evaluation of TCM preparations mainly focused on chemical ingredients. The biological ingredient analysis of TCM preparations is also important because TCM preparations usually contain both plant and animal ingredients, which often include some mis-identified herbal materials, adulterants or even some biological contaminants. For biological ingredient analysis, the efficiency of DNA extraction is an important factor which might affect the accuracy and reliability of identification. The component complexity in TCM preparations is high, and DNA might be destroyed or degraded in different degrees after a series of processing procedures. Therefore, it is necessary to establish an effective protocol for DNA extraction from TCM preparations. In this study, we chose a classical TCM preparation, Liuwei Dihuang Wan （LDW）, as an example to develop a TCM-specific DNA extraction method. An optimized cetyl trimethyl ammonium bromide （CTAB） method （TCM-CTAB） and three commonly-used extraction kits were tested for extraction of DNA from LDW samples. Experimental results indicated that DNA with the highest purity and concentration was obtained by using TCM-CTAB. To further evaluate the different extraction methods, amplification of the second internal transcribed spacer （ITS2） and the chloroplast genome trnL intron was carried out.

Inefficiency of C3H/HeN Mice to Control Chlamydial Lung Infection Correlates with Downregulation of Neutrophil Activation during the Late Stage of Infection

We previously reported that massive infiltration of neutrophils in C3H/HeN （C3H） mice could not efficiently control Chlamydia muridarum （Cm） infection and might contribute to the high susceptibility of these mice to lung infection. To further define the nature of neutrophil responses in C3H mice during chlamydial infection, we examine the expression of adhesion molecules and CDllb related to neutrophils infiltration and activation, respectively, following intranasal Cm infection. The results showed that the expression of selectins （E-selectin, P-selectin and L-selectin）, and intercellular cell adhesion molecule-1 （ICAM-1） in the lung of C3H mice increased more significantly than in C57BL/6 （B6） mice, the more resistant strain. These results correlated well with the massive neutrophils infiltration in C3H mice. In contrast, CDllb expression on peripheral blood and lung neutrophils in C3H mice exhibited a significant reduction compared with B6 mice during the late phage of infection （day 14）. These findings suggest that the high-level expression of adhesion molecules in C3H mice may enhance neutrophils recruitment to the lung, but the decline of CDllb expression on neutrophils may attenuate neutrophil function. Therefore, CDllb down-regulation on neutrophils may contribute to the failure of C3H mice to control chlamydial lung infection.

Type 1 T-cell responses in chlamydial lung infections are associated with local MIP-1a response

Chemokines and their receptors are important mediators of leukocyte trafficking and recruitment and sometimes work as modulators of T-cell responses during infections and inflammation.Modulating the biological activity of chemokines has been found to influence the course of diseases.However,little is known about the role of chemokine responses during chlamydial lung infections.We therefore analyzed the dynamics of multiple chemokines,which are frequently associated with type 1(Th1)T cell immune responses,and their receptors for their expression in the lungs during Chlamydia muridarum(Cm)infections.We also examined the relationship between chemokine responses and the development of Th1 responses as well as the clearance of infection.Our results showed that in parallel with the high levels of gamma interferon(IFN-c)and IL-12 production in the lungs and draining lymph nodes,and the expansion of IFN-c-producing CD4 and CD81 T cells,the production of the cell-related chemokines RANTES,IFN-c-inducible protein-10(IP-10)and macrophage inflammatory protein-1a(MIP-1a)and their receptor CCR1 was elevated in the lung tissues after infection.Interestingly,in a later phase of infection,the expression of RANTES and IP-10 remained elevated but the expression of MIP-1a and CCR1 decreased to a low level,which suggests a closer association with the pattern of Th1 cytokine responses in the process of infection.These results suggest a close association between the MIP-1a response and the Th1-type T-cell responses in chlamydial lung infections .