Background:Atherosclerosis(AS)has been a potentially life-threatening disease worldwide.Alisma decoction(AD)is a famous Chinese formula in treating AS.However,the active components and potential mechanisms remain unkn...Background:Atherosclerosis(AS)has been a potentially life-threatening disease worldwide.Alisma decoction(AD)is a famous Chinese formula in treating AS.However,the active components and potential mechanisms remain unknown.Methods:In this study,the active compositions of AD were analyzed by ultrahigh-performance liquid chromatography quadrupole-orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap-HRMS).The putative targets were predicted and interpreted by a series of bio-informative tools such as Venn,STRING,NetworkAnalyzer,ClusterMarker,and DAVID.Finally,the results were validated by molecular docking using AutoDock.Results:The results showed that alisol A,alisol B,23-o-Acetylalisol B,atractylenolideⅢand atractylenolideⅡwere the major compositions in AD.Thirty targets mainly distributed in three gene clusters were obtained after topological and cluster analysis.KEGG and GO analyses showed that genes in cluster 1 mainly participated in adipocytokine signaling pathway and played an important role in lipid metabolism,while genes in cluster 2 had regulatory actions via cAMP signaling pathway and may protect vascular function from AS,and genes in cluster 3 were closely related to inflammation.Furthermore,RXRA,AGT and CXCL8,the central gene of cluster 1,2 and 3,were verified to be strong binding with some major compositions in AD.Conclusion:The possible mechanisms of AD in the treatment of AS may be closely correlated with the regulation of lipid metabolism,vascular physiology and inflammation.Our study provided a new insight into the anti-AS effect of AD,but more pharmacological experiments should be performed for verification in the future.展开更多
The mortality rate of severe coronavirus disease 2019(COVID-19)is high,and there is no universally accepted method to predict its occurrence.COVID-19 pneumonia can be classified into four categories,such as,asymptomat...The mortality rate of severe coronavirus disease 2019(COVID-19)is high,and there is no universally accepted method to predict its occurrence.COVID-19 pneumonia can be classified into four categories,such as,asymptomatic,mild,moderate,and severe.According to Wu et al.1 data regarding COVID-19 patients in China's Mainland showed that among 44,415 patients who suffered from COVID-19,36,160 patients had mild COVID-19.展开更多
基金This work was supported by National Natural Science Foundation of China,Grant/Award Numbers:82004103Natural Science Foundation of Guangdong Province,Grant/Award Numbers:2019A1515011560+3 种基金Open fund of Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications,Liaoning University of Traditional Chinese Medicine,Grant/Award Numbers:zyzx1901Scientific Research Fund of People's Hospital of Yangjiang,Grant/Award Numbers:G2020001 and G2020002Scientific Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine:Grant/Award Numbers:20201392,20211436 and 20212269Science and Technology Program of Yangjiang Science and Technology Bureau,Grant/Award Numbers:2019.50.25,Science and Technology Program of Yangjiang Health Bureau,Grant/Award Numbers:2019050.
文摘Background:Atherosclerosis(AS)has been a potentially life-threatening disease worldwide.Alisma decoction(AD)is a famous Chinese formula in treating AS.However,the active components and potential mechanisms remain unknown.Methods:In this study,the active compositions of AD were analyzed by ultrahigh-performance liquid chromatography quadrupole-orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap-HRMS).The putative targets were predicted and interpreted by a series of bio-informative tools such as Venn,STRING,NetworkAnalyzer,ClusterMarker,and DAVID.Finally,the results were validated by molecular docking using AutoDock.Results:The results showed that alisol A,alisol B,23-o-Acetylalisol B,atractylenolideⅢand atractylenolideⅡwere the major compositions in AD.Thirty targets mainly distributed in three gene clusters were obtained after topological and cluster analysis.KEGG and GO analyses showed that genes in cluster 1 mainly participated in adipocytokine signaling pathway and played an important role in lipid metabolism,while genes in cluster 2 had regulatory actions via cAMP signaling pathway and may protect vascular function from AS,and genes in cluster 3 were closely related to inflammation.Furthermore,RXRA,AGT and CXCL8,the central gene of cluster 1,2 and 3,were verified to be strong binding with some major compositions in AD.Conclusion:The possible mechanisms of AD in the treatment of AS may be closely correlated with the regulation of lipid metabolism,vascular physiology and inflammation.Our study provided a new insight into the anti-AS effect of AD,but more pharmacological experiments should be performed for verification in the future.
基金This work was supported by the grants from National Natural Science Foundation of China(grant number 81772266)Natural Science Foundation of Guangdong Province(grant number 2017A030311014).We sincerely thank all collaborators who contributed to this study.We sincerely thank all patients and healthy volunteers for participating in this study。
文摘The mortality rate of severe coronavirus disease 2019(COVID-19)is high,and there is no universally accepted method to predict its occurrence.COVID-19 pneumonia can be classified into four categories,such as,asymptomatic,mild,moderate,and severe.According to Wu et al.1 data regarding COVID-19 patients in China's Mainland showed that among 44,415 patients who suffered from COVID-19,36,160 patients had mild COVID-19.
