The Chinese tree shrew(Tupaia belangeri chinensis)has emerged as a promising model for investigating adrenal steroid synthesis,but it is unclear whether the same cells produce steroid hormones and whether their produc...The Chinese tree shrew(Tupaia belangeri chinensis)has emerged as a promising model for investigating adrenal steroid synthesis,but it is unclear whether the same cells produce steroid hormones and whether their production is regulated in the same way as in humans.Here,we comprehensively mapped the cell types and pathways of steroid metabolism in the adrenal gland of Chinese tree shrews using single-cell RNA sequencing,spatial transcriptome analysis,mass spectrometry,and immunohistochemistry.We compared the transcriptomes of various adrenal cell types across tree shrews,humans,macaques,and mice.Results showed that tree shrew adrenal glands expressed many of the same key enzymes for steroid synthesis as humans,including CYP11B2,CYP11B1,CYB5A,and CHGA.Biochemical analysis confirmed the production of aldosterone,cortisol,and dehydroepiandrosterone but not dehydroepiandrosterone sulfate in the tree shrew adrenal glands.Furthermore,genes in adrenal cell types in tree shrews were correlated with genetic risk factors for polycystic ovary syndrome,primary aldosteronism,hypertension,and related disorders in humans based on genome-wide association studies.Overall,this study suggests that the adrenal glands of Chinese tree shrews may consist of closely related cell populations with functional similarity to those of the human adrenal gland.Our comprehensive results(publicly available at http://gxmujyzmolab.cn:16245/scAGMap/)should facilitate the advancement of this animal model for the investigation of adrenal gland disorders.展开更多
Objectives:To explore the level of active aging and correlates among rural elderly in Xiangtan County,China.Methods:A cross-sectional survey was conducted from July to November 2019.A total of 945 rural elderly from 3...Objectives:To explore the level of active aging and correlates among rural elderly in Xiangtan County,China.Methods:A cross-sectional survey was conducted from July to November 2019.A total of 945 rural elderly from 3 towns in Xiangtan County,China,were investigated by using the positive aging evaluation questionnaire(PAEQ),the activity of daily living scale(ADL),and the depression in old-age scale(DIA-S).Results:The average score in the PAEQ among the rural elderly of Xiangtan County was 72.81(range=21-105).A moderate negative correlation was found between the score in the PAEQ and those in the ADL(r=−0.361)and DIA-S(r=−0.495).Symptoms of depression(β:−0.321,P=0.001),number of chronic diseases(β:−0.281,P=0.001),subjective economic status(β:0.239,P=0.001),ADL(β:−0.196,P=0.001),education(β:0.126,P=0.001),number of children(β:0.097,P=0.001),and marital status(β:−0.060,P=0.001)were significantly associated with levels of active aging in rural elderly,whereas gender and age were not independently related to active aging.Conclusions:The level of active aging is at a moderately high level in the Chinese elderly in rural areas in Xiangtan County.The focus group of active aging in rural areas should be elderly people suffering from chronic diseases,physical decline,poor education and economic conditions,childlessness,and those without partners.展开更多
The microstructure in welding heat-affected zones of 5 wt.% manganese steels was studied, and its effect on impact toughness was analyzed. The simulated coarse-grained heat-affected zone (CGHAZ) had the lowest impact ...The microstructure in welding heat-affected zones of 5 wt.% manganese steels was studied, and its effect on impact toughness was analyzed. The simulated coarse-grained heat-affected zone (CGHAZ) had the lowest impact toughness of ~39 J at — 40℃ because of coarse-grained structure and least volume fraction of retained austenite (RA) of 1.2 vol.%. The impact toughness of simulated intercritical heat-affected zone (ICHAZ) and fine-grained heat-affected zone (FGHAZ) were ~165 and ~45 J, respectively, at — 40℃. The effective grain size of simulated FGHAZ was smaller than that of the simulated ICHAZ. Furthermore, microstructural investigation revealed that the simulated FGHAZ and ICHAZ had similarity in volume fraction and stability of RA. However, tempered martensite was present in ICHAZ and absent in FGHAZ. It is proposed that the presence of tempered martensite contributed to good impact toughness in simulated ICHAZ.展开更多
基金supported by the Key Research and Development Program of Guangxi(2021AB13014)Major Project of Guangxi Innovation Driven(AA18118016)+7 种基金National Key Research and Development Program of China(2017YFC0908000)Natural Key Research and Development Project(2020YFA0113200)National Natural Science Foundation of China(81770759,82060145,31970814)Natural Science Foundation of Guangxi Zhuang Autonomous Region(2021JJA140912)Advanced Innovation Teams and Xinghu Scholars Program of Guangxi Medical University,Guangxi Key Laboratory for Genomic and Personalized Medicine(19-050-22,19-185-33,20-065-33,22-35-17)Major Project of Scientific Research and Technology Development Plan of Nanning(20221023)Guangxi Natural Science Foundation(2022GXNSFAA035641)Self-funded Project of Health Commission of Guangxi Zhuang Autonomous Region(Z-A20230620)。
文摘The Chinese tree shrew(Tupaia belangeri chinensis)has emerged as a promising model for investigating adrenal steroid synthesis,but it is unclear whether the same cells produce steroid hormones and whether their production is regulated in the same way as in humans.Here,we comprehensively mapped the cell types and pathways of steroid metabolism in the adrenal gland of Chinese tree shrews using single-cell RNA sequencing,spatial transcriptome analysis,mass spectrometry,and immunohistochemistry.We compared the transcriptomes of various adrenal cell types across tree shrews,humans,macaques,and mice.Results showed that tree shrew adrenal glands expressed many of the same key enzymes for steroid synthesis as humans,including CYP11B2,CYP11B1,CYB5A,and CHGA.Biochemical analysis confirmed the production of aldosterone,cortisol,and dehydroepiandrosterone but not dehydroepiandrosterone sulfate in the tree shrew adrenal glands.Furthermore,genes in adrenal cell types in tree shrews were correlated with genetic risk factors for polycystic ovary syndrome,primary aldosteronism,hypertension,and related disorders in humans based on genome-wide association studies.Overall,this study suggests that the adrenal glands of Chinese tree shrews may consist of closely related cell populations with functional similarity to those of the human adrenal gland.Our comprehensive results(publicly available at http://gxmujyzmolab.cn:16245/scAGMap/)should facilitate the advancement of this animal model for the investigation of adrenal gland disorders.
基金Hunan Social and Social Science Achievement Review Committee(No.XSP19YBC050)Hunan Provincial Health Commission(No.20200094).
文摘Objectives:To explore the level of active aging and correlates among rural elderly in Xiangtan County,China.Methods:A cross-sectional survey was conducted from July to November 2019.A total of 945 rural elderly from 3 towns in Xiangtan County,China,were investigated by using the positive aging evaluation questionnaire(PAEQ),the activity of daily living scale(ADL),and the depression in old-age scale(DIA-S).Results:The average score in the PAEQ among the rural elderly of Xiangtan County was 72.81(range=21-105).A moderate negative correlation was found between the score in the PAEQ and those in the ADL(r=−0.361)and DIA-S(r=−0.495).Symptoms of depression(β:−0.321,P=0.001),number of chronic diseases(β:−0.281,P=0.001),subjective economic status(β:0.239,P=0.001),ADL(β:−0.196,P=0.001),education(β:0.126,P=0.001),number of children(β:0.097,P=0.001),and marital status(β:−0.060,P=0.001)were significantly associated with levels of active aging in rural elderly,whereas gender and age were not independently related to active aging.Conclusions:The level of active aging is at a moderately high level in the Chinese elderly in rural areas in Xiangtan County.The focus group of active aging in rural areas should be elderly people suffering from chronic diseases,physical decline,poor education and economic conditions,childlessness,and those without partners.
基金Authors gratefully acknowledge the support from National Natural Science Foundation of China (No. 2015AA03A501).
文摘The microstructure in welding heat-affected zones of 5 wt.% manganese steels was studied, and its effect on impact toughness was analyzed. The simulated coarse-grained heat-affected zone (CGHAZ) had the lowest impact toughness of ~39 J at — 40℃ because of coarse-grained structure and least volume fraction of retained austenite (RA) of 1.2 vol.%. The impact toughness of simulated intercritical heat-affected zone (ICHAZ) and fine-grained heat-affected zone (FGHAZ) were ~165 and ~45 J, respectively, at — 40℃. The effective grain size of simulated FGHAZ was smaller than that of the simulated ICHAZ. Furthermore, microstructural investigation revealed that the simulated FGHAZ and ICHAZ had similarity in volume fraction and stability of RA. However, tempered martensite was present in ICHAZ and absent in FGHAZ. It is proposed that the presence of tempered martensite contributed to good impact toughness in simulated ICHAZ.
