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木犀草素通过调节细胞凋亡和炎症发挥对内毒素性所致急性肾损伤的保护作用 被引量:1
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作者 肖辉 韩星 +1 位作者 金洪杰 刘潇 《药物流行病学杂志》 CAS 2023年第7期766-773,共8页
目的探讨木犀草素对急性肾损伤的保护作用。方法通过脂多糖(LPS构建小鼠内毒素性所致的急性肾损伤模型,使用木犀草素对肾损伤小鼠进行治疗;给小鼠尾静脉注射miR-30c-5p antagomir以敲低miR-30c-5p的表达。动物分组如下:正常对照组、LPS... 目的探讨木犀草素对急性肾损伤的保护作用。方法通过脂多糖(LPS构建小鼠内毒素性所致的急性肾损伤模型,使用木犀草素对肾损伤小鼠进行治疗;给小鼠尾静脉注射miR-30c-5p antagomir以敲低miR-30c-5p的表达。动物分组如下:正常对照组、LPS组、木犀草素治疗组(LPS+Lu组)、木犀草素治疗的阴性对照组(LPS+DMSO组)、miR-30c-5p表达敲低组(antagomir 30c-5p组)、miR-30c-5p表达敲低对照组(antagomir NC组)。采用自动化学分析仪测定小鼠血清肌酐和尿素氮水平,通过HE染色观察肾组织病理损伤。采用ELISA试剂盒检测小鼠血清胱抑素C(Cys-C)、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-4水平,通过TUNEL染色和Western blot检测细胞凋亡。结果与正常对照组相比,LPS组小鼠急性肾损伤病理情况严重,血清肌酐、尿素氮和Cys-C、促炎因子IL-6、TNF-α、促凋亡因子B淋巴细胞瘤-2基因(Bcl-2)相关X蛋白(Bax)的表达水平显著升高(P<0.05),而抗炎因子IL-4、抗凋亡因子Bcl-2、miR-30c-5p水平显著下降(P<0.05)。与LPS组相比,LPS+Lu组肾损伤病理情况减轻,血清肌酐、尿素氮和Cys-C、IL-6、TNF-α、Bax的表达水平显著降低,而IL-4、Bcl-2、miR-30c-5p水平显著上升(P<0.05)。与antagomir NC组小鼠相比,antagomir 30c-5p组小鼠促炎因子水平及细胞凋亡率显著上升(P<0.05)。结论木犀草素通过上调miR-30c-5p表达抑制炎症和细胞凋亡从而保护急性肾损伤小鼠。 展开更多
关键词 木犀草素 急性肾损伤 内毒素性 细胞凋亡 炎症 保护
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Posterior mediastinal extralobar pulmonary sequestration misdiagnosed as a neurogenic tumor:A case report
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作者 hong-jie jin Yue Yu +1 位作者 Wei He Yun Han 《World Journal of Clinical Cases》 SCIE 2022年第26期9340-9347,共8页
BACKGROUND Pulmonary sequestration-both intralobar and extralobar-is a rare congenital developmental malformation.Extralobar pulmonary sequestrations(EPS)have their own pleura but are separated from the bronchus and u... BACKGROUND Pulmonary sequestration-both intralobar and extralobar-is a rare congenital developmental malformation.Extralobar pulmonary sequestrations(EPS)have their own pleura but are separated from the bronchus and usually occur in the left lung.They are mainly found mainly between the lower lobe and the mediastinum.EPS is rarely found within the mediastinum itself,even rarer so in the posterior mediastinum.CASE SUMMARY We report the case of a 27-year-old man who was misdiagnosed with a neurogenic tumor based on preoperative contrast-enhanced computed tomography(CT)and magnetic resonance imaging findings.Contrast-enhanced chest CT revealed a posterior mediastinal mass measuring 1.2 cm×1.4 cm×3.3 cm,which consisted of some cystic areas and showed slight enhancement.The mass was in the 11th paravertebral region and attached to the 11th thoracic vertebra behind the descending aorta in the posterior mediastinum.An arteriole originating from the intercostal artery and a vein originating directly from the hemiazygos vein were found in the pedicle of the mass.The mass was resected in a uniport videoassisted thoracoscopic surgery.During the operation,the pyramid-shaped mass appeared well-encapsulated.Postoperative histopathology established a diagnosis of EPS.One month later,a follow-up CT of the thorax showed good recovery.CONCLUSION Although EPS rarely occurs in the posterior mediastinum,its diagnosis should be considered when posterior mediastinal tumors are suspected. 展开更多
关键词 Posterior mediastinal mass Pulmonary sequestration Extralobar pulmonary sequestration Congenital malformation Video-assisted thoracoscopic surgery Case report
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