Objective:To evaluate the value of procalcitonin (PCT) content for adjuvant diagnosis of neonatal septicemia and assessment of its severity.Methods: A total of 100 newborns who were diagnosed with septicemia in the Fi...Objective:To evaluate the value of procalcitonin (PCT) content for adjuvant diagnosis of neonatal septicemia and assessment of its severity.Methods: A total of 100 newborns who were diagnosed with septicemia in the First People's Hospital of Xianyang City between January 2017 and January 2018 were selected as the septicemia group of the research, and the newborns who were born in the First People's Hospital of Xianyang City during the same period, with the general data matched with those of septicemia group and without neonatal diseases after born were selected as the control group. Serum was collected to determine the contents of PCT, inflammatory cytokines and immune cytokines, and peripheral blood was collected to determine the expression intensity of inflammatory signaling molecules and immune transcription factors.Results: Serum PCT, TNF-α, HMGB1, ICAM-1, IL-10 and IL-17 contents as well as peripheral blood TLR4, NF-κB, MPO, RORγt and FOXP3 expression intensity of septicemia group were significantly higher than those of control group whereas peripheral blood HLA-DR expression intensity was lower than that of control group;peripheral blood TLR4, NF-κB, MPO, RORγt and FOXP3 expression intensity as well as serum TNF-α, HMGB1, ICAM-1, IL-10 and IL-17 contents of septicemia group of newborns with high PCT were significantly higher than those of newborns with low PCT whereas peripheral blood HLA-DR expression intensity was lower than that of newborns with low PCT.Conclusion: The increase of PCT in serum of newborns with septicemia is related to the change of inflammatory response and immune response, and its content detection is valuable for assessing the severity of the disease.展开更多
文摘Objective:To evaluate the value of procalcitonin (PCT) content for adjuvant diagnosis of neonatal septicemia and assessment of its severity.Methods: A total of 100 newborns who were diagnosed with septicemia in the First People's Hospital of Xianyang City between January 2017 and January 2018 were selected as the septicemia group of the research, and the newborns who were born in the First People's Hospital of Xianyang City during the same period, with the general data matched with those of septicemia group and without neonatal diseases after born were selected as the control group. Serum was collected to determine the contents of PCT, inflammatory cytokines and immune cytokines, and peripheral blood was collected to determine the expression intensity of inflammatory signaling molecules and immune transcription factors.Results: Serum PCT, TNF-α, HMGB1, ICAM-1, IL-10 and IL-17 contents as well as peripheral blood TLR4, NF-κB, MPO, RORγt and FOXP3 expression intensity of septicemia group were significantly higher than those of control group whereas peripheral blood HLA-DR expression intensity was lower than that of control group;peripheral blood TLR4, NF-κB, MPO, RORγt and FOXP3 expression intensity as well as serum TNF-α, HMGB1, ICAM-1, IL-10 and IL-17 contents of septicemia group of newborns with high PCT were significantly higher than those of newborns with low PCT whereas peripheral blood HLA-DR expression intensity was lower than that of newborns with low PCT.Conclusion: The increase of PCT in serum of newborns with septicemia is related to the change of inflammatory response and immune response, and its content detection is valuable for assessing the severity of the disease.
